LOCUS       pGG-UmpRbcS-BleR-P2A-mTagBFP-UmtRbcS        5016 bp    DNA     circular UNA 11-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.pk-cgtpvim
VERSION     urn.local.jonasblomme.pk-cgtpvim
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     promoter        1..546
                     /regulatory_class="promoter"
                     /note="Ulva RbcS promoter"
                     /label="UmpRbcS"
     CDS             join(551..558,1056..1419)
                     /gene="ble"
                     /note="derived from Streptoalloteichus hindustanus,
                     adapted to Chlamydomonas reinhardtii and Ulva mutabilis
                     codon usage"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="ABY50194.1"
                     /db_xref="GI:163962062"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD"
     intron          559..1055
                     /label="RbcsI"
     CDS             1420..1485
                     /label="P2A"
     Linker          1486..1515
                     /origin="C-linker-D (53bp linear)Fragment: from BsaI cut
                     at 12^13 to BsaI cut at 40^41, 28bpOrientation:
                     originalPlus strand 5': no changePlus strand 3': no
                     changeMinus strand 5': no changeMinus strand 3': no
                     change"
     CDS             1522..2220
                     /SerialCloner_Color="&hFF0000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mTagBFP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; mTagBFP
                     (<a
                     href=""urn:local:jonasblomme:2d-cnsqc2r"">mTagBFP2</a>)"
                     /modified_by="jonasblomme"
                     /label="mTagBFP"
     terminator      2228..2616
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
                     /label="UmtRbcS"
     Linker          2621..2669
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(2706..2726)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2787..2837
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2838..3647
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3794..4382
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4981..5001
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 cgttgttcgt acacaaggat gtcgcttgtg gctttttcgc tcggctttcc aggagggcag
       61 tccagatacc atgcggccgc cgtgtccatc actcgcgctg cagctgatgt gggcagatac
      121 tgcactaatc tgccgcacga cgcgtcggcc tgagtcggcg gacaccggag gcttgctgga
      181 gtactggcac aagtgtgcgt gtgattgcca gaaacagcaa cccactcact agatataccg
      241 ctccaataat gggttcatgt gcaagctcac tcaataacga aacgcttcac tattgtgcac
      301 gtgccggggt tgcttgggta tggcgtgcca tacgggttct gcacatgtca cccacgtggg
      361 tgtggcacga gatatcgagg tggccatgta aaaggcattt gctagagggc agctcgtcca
      421 tgccgctcga gcacaccgag tgcgcaccat aaatgcagca catgcctgtg cagcctatct
      481 cctgtctctg ttacatccca ccacctcctc acatatcccc ttccaccctt aaaccgcaca
      541 gcagacaaca atggccaagt gcgcatcgcg tgtcaatgct ggcgctgctg acgtccgctg
      601 cctaaactgc atgaaagctc ctgcagtcgc aagtagtcct tttaacttgt tgtatcggaa
      661 caaatggttg tagtctgtgc cgtgtgacga tcttgagccg cctggtcggc aggtccaatg
      721 tcggcctgga ccctgcacac cctgcccacc ctgccgctgc tcacccgttc acgaccccac
      781 aggtgctcgc agcagtaatt gtcgatagat agcaactgtg ttctgactat tctttgacct
      841 tccgcatttg ggactgcggc ggcacaccta ctatccgcaa tgcacaccct ggtgctggta
      901 ctgcctgcct ttccgcgcga gcccacacca tgcaggcgca tagtaatgga ctgtgattga
      961 tttaggcaac tgtatgaaac ctcttcatcg tgccttggtt gcttgcgata ccctcacgtg
     1021 agttcatatg accaacgctg gcacgacccc tgcaggatca ccagcgccgt tccggtgctc
     1081 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     1141 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     1201 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     1261 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     1321 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     1381 gccggcaact gcgtgcactt cgtggccgag gagcaggacg gcagcggcgc tactaatttt
     1441 tctcttctta agcaagctgg agatgttgaa gaaaatcctg gacctggctc cgtattcagt
     1501 cgactggtac caacatcagc tatgagcgag ctgatcaagg agaacatgca catgaagctg
     1561 tacatggagg gcaccgtgga caaccaccac ttcaagtgca ccagcgaggg cgagggcaag
     1621 ccctacgagg gcacccagac catgcgcatc aaggtggtgg agggcggccc cctgcccttc
     1681 gccttcgaca tcctggccac ctcgttcctg tacggcagca agaccttcat caaccacacc
     1741 cagggcatcc ccgacttctt caagcagagc ttccccgagg gcttcacctg ggagcgcgtg
     1801 accacctacg aggacggcgg cgtgctgacc gccacccagg acaccagcct ccaggacggc
     1861 tgcctgatct acaacgtgaa gatccgcggc gtgaacttca ccagcaacgg ccccgtgatg
     1921 cagaagaaga ccctgggctg ggaggccttc accgagacgc tgtaccccgc tgacggcggc
     1981 ctggagggcc gcaacgacat ggccctgaag ctggtgggcg gcagccacct gatcgccaac
     2041 atcaagacca cctaccgcag caagaagccc gccaagaacc tgaagatgcc cggcgtgtac
     2101 tacgtggact accgcctgga gcgcatcaag gaggccaaca acgagacgta cgtggagcag
     2161 cacgaggtgg ccgtggcccg ctactgcgac ctgcccagca agctgggcca caagctgaac
     2221 taactgcgca gtcaggcaga gtcgttgttg ggtgtatggt tgcacaaaac tagacctcga
     2281 gctggtcggt tagaaagaga tgcttgtgcg actggatgta acaggcacaa tccgaggaaa
     2341 atgcaacaga aacgttatga tgtaacatct gagcacagtc aataaaattg catgttgtat
     2401 gccgcacaca aaagtgttgt taaacatgcg atgtggttgt caaatcatta ttcgcacaac
     2461 catggaccga acctctggca cgctgcatac gctgtattca ttcacatcat gttttctact
     2521 tgtcacatgc tctgcaccaa gtaactccga ttagttaggt tcgcacatgt catcggagtg
     2581 gaccacttcg aatcatttgg ttcgaagata aaccggacta taagctaggt agtaactagt
     2641 gcgactgtct agaatcgcgt atgcgagtgt atgagctcga tatcccctat agtgagtcgt
     2701 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     2761 acattgcaca agataaaata atatcatcat gaacaataaa actgtctgct tacataaaca
     2821 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
     2881 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     2941 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     3001 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     3061 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     3121 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag
     3181 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     3241 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     3301 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     3361 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     3421 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     3481 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     3541 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     3601 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     3661 attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac
     3721 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     3781 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3841 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3901 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3961 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4021 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4081 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4141 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4201 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4261 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4321 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4381 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4441 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     4501 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     4561 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     4621 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     4681 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     4741 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     4801 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     4861 caaacaacag ataaaacgaa aggcccagac ttccgactga gcctttcgtt ttatttgatg
     4921 cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt
     4981 gtaaaacgac ggccagtctt aagctcgggc ccacct
//
