LOCUS       pGG-UmpRbcS-BleR-E2A-mTagBFP-UmtRbcS        5019 bp    DNA     circular UNA 11-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.pi-cgtpvim
VERSION     urn.local.jonasblomme.pi-cgtpvim
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     promoter        1..546
                     /regulatory_class="promoter"
                     /note="Ulva RbcS promoter"
                     /label="UmpRbcS"
     CDS             join(551..558,1056..1419)
                     /gene="ble"
                     /note="derived from Streptoalloteichus hindustanus,
                     adapted to Chlamydomonas reinhardtii and Ulva mutabilis
                     codon usage"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="ABY50194.1"
                     /db_xref="GI:163962062"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD"
     intron          559..1055
                     /label="RbcsI"
     CDS             1420..1488
                     /label="E2A"
     Linker          1489..1518
                     /origin="C-linker-D (53bp linear)Fragment: from BsaI cut
                     at 12^13 to BsaI cut at 40^41, 28bpOrientation:
                     originalPlus strand 5': no changePlus strand 3': no
                     changeMinus strand 5': no changeMinus strand 3': no
                     change"
     CDS             1525..2223
                     /SerialCloner_Color="&hFF0000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mTagBFP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; mTagBFP
                     (<a
                     href=""urn:local:jonasblomme:2d-cnsqc2r"">mTagBFP2</a>)"
                     /modified_by="jonasblomme"
                     /label="mTagBFP"
     terminator      2231..2619
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
                     /modified_by="jonasblomme"
                     /label="UmtRbcS"
     Linker          2624..2672
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(2709..2729)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2790..2840
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2841..3650
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3797..4385
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4984..5004
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 cgttgttcgt acacaaggat gtcgcttgtg gctttttcgc tcggctttcc aggagggcag
       61 tccagatacc atgcggccgc cgtgtccatc actcgcgctg cagctgatgt gggcagatac
      121 tgcactaatc tgccgcacga cgcgtcggcc tgagtcggcg gacaccggag gcttgctgga
      181 gtactggcac aagtgtgcgt gtgattgcca gaaacagcaa cccactcact agatataccg
      241 ctccaataat gggttcatgt gcaagctcac tcaataacga aacgcttcac tattgtgcac
      301 gtgccggggt tgcttgggta tggcgtgcca tacgggttct gcacatgtca cccacgtggg
      361 tgtggcacga gatatcgagg tggccatgta aaaggcattt gctagagggc agctcgtcca
      421 tgccgctcga gcacaccgag tgcgcaccat aaatgcagca catgcctgtg cagcctatct
      481 cctgtctctg ttacatccca ccacctcctc acatatcccc ttccaccctt aaaccgcaca
      541 gcagacaaca atggccaagt gcgcatcgcg tgtcaatgct ggcgctgctg acgtccgctg
      601 cctaaactgc atgaaagctc ctgcagtcgc aagtagtcct tttaacttgt tgtatcggaa
      661 caaatggttg tagtctgtgc cgtgtgacga tcttgagccg cctggtcggc aggtccaatg
      721 tcggcctgga ccctgcacac cctgcccacc ctgccgctgc tcacccgttc acgaccccac
      781 aggtgctcgc agcagtaatt gtcgatagat agcaactgtg ttctgactat tctttgacct
      841 tccgcatttg ggactgcggc ggcacaccta ctatccgcaa tgcacaccct ggtgctggta
      901 ctgcctgcct ttccgcgcga gcccacacca tgcaggcgca tagtaatgga ctgtgattga
      961 tttaggcaac tgtatgaaac ctcttcatcg tgccttggtt gcttgcgata ccctcacgtg
     1021 agttcatatg accaacgctg gcacgacccc tgcaggatca ccagcgccgt tccggtgctc
     1081 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     1141 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     1201 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     1261 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     1321 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     1381 gccggcaact gcgtgcactt cgtggccgag gagcaggacg gcagcggcca gtgcacgaac
     1441 tacgccctgc tgaagctggc gggcgacgtg gagagcaacc cgggcccggg ctccgtattc
     1501 agtcgactgg taccaacatc agctatgagc gagctgatca aggagaacat gcacatgaag
     1561 ctgtacatgg agggcaccgt ggacaaccac cacttcaagt gcaccagcga gggcgagggc
     1621 aagccctacg agggcaccca gaccatgcgc atcaaggtgg tggagggcgg ccccctgccc
     1681 ttcgccttcg acatcctggc cacctcgttc ctgtacggca gcaagacctt catcaaccac
     1741 acccagggca tccccgactt cttcaagcag agcttccccg agggcttcac ctgggagcgc
     1801 gtgaccacct acgaggacgg cggcgtgctg accgccaccc aggacaccag cctccaggac
     1861 ggctgcctga tctacaacgt gaagatccgc ggcgtgaact tcaccagcaa cggccccgtg
     1921 atgcagaaga agaccctggg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc
     1981 ggcctggagg gccgcaacga catggccctg aagctggtgg gcggcagcca cctgatcgcc
     2041 aacatcaaga ccacctaccg cagcaagaag cccgccaaga acctgaagat gcccggcgtg
     2101 tactacgtgg actaccgcct ggagcgcatc aaggaggcca acaacgagac gtacgtggag
     2161 cagcacgagg tggccgtggc ccgctactgc gacctgccca gcaagctggg ccacaagctg
     2221 aactaactgc gcagtcaggc agagtcgttg ttgggtgtat ggttgcacaa aactagacct
     2281 cgagctggtc ggttagaaag agatgcttgt gcgactggat gtaacaggca caatccgagg
     2341 aaaatgcaac agaaacgtta tgatgtaaca tctgagcaca gtcaataaaa ttgcatgttg
     2401 tatgccgcac acaaaagtgt tgttaaacat gcgatgtggt tgtcaaatca ttattcgcac
     2461 aaccatggac cgaacctctg gcacgctgca tacgctgtat tcattcacat catgttttct
     2521 acttgtcaca tgctctgcac caagtaactc cgattagtta ggttcgcaca tgtcatcgga
     2581 gtggaccact tcgaatcatt tggttcgaag ataaaccgga ctataagcta ggtagtaact
     2641 agtgcgactg tctagaatcg cgtatgcgag tgtatgagct cgatatcccc tatagtgagt
     2701 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
     2761 gttacattgc acaagataaa ataatatcat catgaacaat aaaactgtct gcttacataa
     2821 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
     2881 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     2941 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     3001 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     3061 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     3121 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt
     3181 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     3241 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     3301 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     3361 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
     3421 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     3481 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     3541 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     3601 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     3661 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat
     3721 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     3781 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3841 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3901 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3961 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4021 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4081 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4141 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4201 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4261 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4321 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4381 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4441 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4501 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4561 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4621 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     4681 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     4741 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     4801 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     4861 cgacaaacaa cagataaaac gaaaggccca gacttccgac tgagcctttc gttttatttg
     4921 atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac
     4981 gttgtaaaac gacggccagt cttaagctcg ggcccacct
//
