LOCUS       pGG-UmpRbcS-BleR-E2A-mCherry-UmtRbcS        5028 bp    DNA     circular UNA 11-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.pa-cgtpvik
VERSION     urn.local.jonasblomme.pa-cgtpvik
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     promoter        1..546
                     /regulatory_class="promoter"
                     /note="Ulva RbcS promoter"
                     /label="UmpRbcS"
     CDS             join(551..558,1056..1419)
                     /gene="ble"
                     /note="derived from Streptoalloteichus hindustanus,
                     adapted to Chlamydomonas reinhardtii and Ulva mutabilis
                     codon usage"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="ABY50194.1"
                     /db_xref="GI:163962062"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD"
     intron          559..1055
                     /label="RbcsI"
     CDS             1420..1488
                     /label="E2A"
     Linker          1489..1518
                     /origin="C-linker-D (53bp linear)Fragment: from BsaI cut
                     at 12^13 to BsaI cut at 40^41, 28bpOrientation:
                     originalPlus strand 5': no changePlus strand 3': no
                     changeMinus strand 5': no changeMinus strand 3': no
                     change"
     CDS             1525..2232
                     /SerialCloner_Color="&hFF0000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:jonasblomme:2i-cnsqdta"">mCherry</a>"
                     /modified_by="jonasblomme"
                     /label="mCherry"
     terminator      2240..2628
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
                     /label="UmtRbcS"
     Linker          2633..2681
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(2718..2738)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2799..2849
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2850..3659
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3806..4394
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4993..5013
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 cgttgttcgt acacaaggat gtcgcttgtg gctttttcgc tcggctttcc aggagggcag
       61 tccagatacc atgcggccgc cgtgtccatc actcgcgctg cagctgatgt gggcagatac
      121 tgcactaatc tgccgcacga cgcgtcggcc tgagtcggcg gacaccggag gcttgctgga
      181 gtactggcac aagtgtgcgt gtgattgcca gaaacagcaa cccactcact agatataccg
      241 ctccaataat gggttcatgt gcaagctcac tcaataacga aacgcttcac tattgtgcac
      301 gtgccggggt tgcttgggta tggcgtgcca tacgggttct gcacatgtca cccacgtggg
      361 tgtggcacga gatatcgagg tggccatgta aaaggcattt gctagagggc agctcgtcca
      421 tgccgctcga gcacaccgag tgcgcaccat aaatgcagca catgcctgtg cagcctatct
      481 cctgtctctg ttacatccca ccacctcctc acatatcccc ttccaccctt aaaccgcaca
      541 gcagacaaca atggccaagt gcgcatcgcg tgtcaatgct ggcgctgctg acgtccgctg
      601 cctaaactgc atgaaagctc ctgcagtcgc aagtagtcct tttaacttgt tgtatcggaa
      661 caaatggttg tagtctgtgc cgtgtgacga tcttgagccg cctggtcggc aggtccaatg
      721 tcggcctgga ccctgcacac cctgcccacc ctgccgctgc tcacccgttc acgaccccac
      781 aggtgctcgc agcagtaatt gtcgatagat agcaactgtg ttctgactat tctttgacct
      841 tccgcatttg ggactgcggc ggcacaccta ctatccgcaa tgcacaccct ggtgctggta
      901 ctgcctgcct ttccgcgcga gcccacacca tgcaggcgca tagtaatgga ctgtgattga
      961 tttaggcaac tgtatgaaac ctcttcatcg tgccttggtt gcttgcgata ccctcacgtg
     1021 agttcatatg accaacgctg gcacgacccc tgcaggatca ccagcgccgt tccggtgctc
     1081 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     1141 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     1201 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     1261 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     1321 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     1381 gccggcaact gcgtgcactt cgtggccgag gagcaggacg gcagcggcca gtgcacgaac
     1441 tacgccctgc tgaagctggc gggcgacgtg gagagcaacc cgggcccggg ctccgtattc
     1501 agtcgactgg taccaacatc agctatggtg tccaagggcg aggaggacaa catggccatc
     1561 atcaaggagt tcatgcgctt caaggtgcac atggagggca gcgtgaacgg ccacgagttc
     1621 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     1681 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgagccccca gttcatgtac
     1741 ggcagcaagg cctacgtgaa gcaccccgcc gacatccccg actacctgaa gctgagcttc
     1801 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     1861 acccaggaca gcagcctcca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     1921 aacttcccca gcgacggccc cgtgatgcag aagaagacca tgggctggga ggccagcagc
     1981 gagcgcatgt accccgagga cggcgccctg aagggcgaga tcaagcagcg cctgaagctg
     2041 aaggacggcg gccactacga cgccgaggtg aagaccacct acaaggccaa gaagcccgtg
     2101 cagctgcccg gcgcctacaa cgtgaacatc aagctggaca tcaccagcca caacgaggac
     2161 tacaccatcg tggagcagta cgagcgcgct gagggccgcc acagcaccgg cggcatggac
     2221 gagctgtaca agtaactgcg cagtcaggca gagtcgttgt tgggtgtatg gttgcacaaa
     2281 actagacctc gagctggtcg gttagaaaga gatgcttgtg cgactggatg taacaggcac
     2341 aatccgagga aaatgcaaca gaaacgttat gatgtaacat ctgagcacag tcaataaaat
     2401 tgcatgttgt atgccgcaca caaaagtgtt gttaaacatg cgatgtggtt gtcaaatcat
     2461 tattcgcaca accatggacc gaacctctgg cacgctgcat acgctgtatt cattcacatc
     2521 atgttttcta cttgtcacat gctctgcacc aagtaactcc gattagttag gttcgcacat
     2581 gtcatcggag tggaccactt cgaatcattt ggttcgaaga taaaccggac tataagctag
     2641 gtagtaacta gtgcgactgt ctagaatcgc gtatgcgagt gtatgagctc gatatcccct
     2701 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     2761 atctctgatg ttacattgca caagataaaa taatatcatc atgaacaata aaactgtctg
     2821 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc
     2881 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     2941 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt
     3001 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     3061 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     3121 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat
     3181 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     3241 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     3301 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     3361 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     3421 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     3481 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     3541 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     3601 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     3661 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
     3721 caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     3781 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3841 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3901 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3961 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     4021 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4081 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4141 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4201 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4261 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4321 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4381 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4441 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4501 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4561 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     4621 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     4681 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     4741 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     4801 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     4861 agcgttcacc gacaaacaac agataaaacg aaaggcccag acttccgact gagcctttcg
     4921 ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc
     4981 agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gcccacct
//
