LOCUS pGG-pUM140_0016-BleR-F2A-mCherry-UmtRbcS 6422 bp DNA circular UNA 11-APR-2023
DEFINITION .
ACCESSION urn.local.jonasblomme.p7-cgtpvik
VERSION urn.local.jonasblomme.p7-cgtpvik
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/.
FEATURES Location/Qualifiers
promoter 1..1934
/modified_by="jonasblomme"
/label="pUM140_0016"
CDS join(1939..1946,2444..2807)
/gene="ble"
/note="derived from Streptoalloteichus hindustanus,
adapted to Chlamydomonas reinhardtii and Ulva mutabilis
codon usage"
/codon_start=1
/transl_table=11
/product="bleomycin resistance protein"
/protein_id="ABY50194.1"
/db_xref="GI:163962062"
/translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
GREFALRDPAGNCVHFVAEEQD"
intron 1947..2443
/label="RbcsI"
CDS 2808..2882
/label="F2A"
Linker 2883..2912
/origin="C-linker-D (53bp linear)Fragment: from BsaI cut
at 12^13 to BsaI cut at 40^41, 28bpOrientation:
originalPlus strand 5': no changePlus strand 3': no
changeMinus strand 5': no changeMinus strand 3': no
change"
CDS 2919..3626
/SerialCloner_Color="&hFF0000"
/SerialCloner_Show="True"
/SerialCloner_Protect="True"
/SerialCloner_Arrow="True"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="mCherry"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; mCherry"
/modified_by="jonasblomme"
/label="mCherry"
terminator 3634..4022
/regulatory_class="terminator"
/note="Ulva RbcS terminator"
/label="UmtRbcS"
Linker 4027..4075
/created_by="wacae"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Linker II"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II"
/label="Linker II"
primer complement(4112..4132)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13R"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
(pCDB-Cas9 - GWM13R)"
/label="GWM13R"
promoter 4193..4243
/created_by="chgai"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 4244..5053
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
rep_origin 5200..5788
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 6387..6407
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13F"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
(pCDB-Cas9 - GWM13F)"
/label="GWM13F"
ORIGIN
1 tgccgaattc gcagtgaacc cgcatggcag ggtggtcaca ggtgtccttt ggctgcacag
61 taacatgacc gtcatgcatg ggacagaggt tgtgagcagc atgcgtcgat gcatacatgc
121 gcatgtagca tgcagcagtg agctccagag tggaaagctg gcccgctgtc gtgtgtccgg
181 accgacaggc caggctctgg actgggccat agtcagcagg gcagaggtac tatgtctagc
241 gccttcctgg gtgtgcagct ttgccttgcc atgcttggac atgaatgagt ggggcgccag
301 gcacttacaa gagccacctc gtggtgtggg catatggcag aggggcaccc atcagtcgtg
361 tgccattcag accgtgacga agcaatcaat gactgtgctt gtccgggaga aagtctgttg
421 tgaaatttgc tgtgatctag gggccaatgt ggcagtcagg aatggctaga gatgctgcta
481 gtgtcgtgca tacctggtgt actgccaaca gcctcacctg accatggtgc ttgtgtttaa
541 agcaagcatg ccacaagtcg gcatgtaggc atacggcaag agctacacag ttggctgttg
601 acctgtggat gcgtgaaaca gaagtttgaa tcgtgccctt tcgcgttgat gcaccctgtg
661 tgggagccag gctggtgtgt gtggaagcca ggctggtggc acacctgtgt gacctggagg
721 tggacgaccg ggtggcgagg caagtagcag tttcatcaaa gaaacacaag ctgatgcatc
781 cgcgatagta gcacgattct aatgtgtgca cgcaaacgca tcggcccaac tggagcaggg
841 gatgcgcagc agcggccgct ggttccacga actcgaagcg caaagagcag tccgctgtga
901 ccagcattcc gctagtgtca gggacgatat gaatacgggt tgggcacatt atgtttgggt
961 cgaggtcgcc atggcggacg tgcaagcggc tgtcgatcac gcctatcgga gatgcccagc
1021 gcctgcatcg atatgcaacc agctctgcac cagcgcagca atacgacagc tgcagccgat
1081 ggcctcctag acactgtgca acaacaaaaa acaattggac cgtgacggca caggtgtcga
1141 acacaggggc tgagagagga ctgcctgtgc ttgtgctcca gtgctcatac gccatactga
1201 tacgcctcca ccgatggcct cactgcctgc ggacactggc agacgctgtc gtttggatgt
1261 cgcatcgcca tcttcagtga tgttgtggcg ttcggcagga tgtacgcatg agcgattctt
1321 ctaaacgagc agtggtggca catcagcggc tccctcaaac cacaatgccg gcttggaagg
1381 tagagcatct gctcggcaag ccgacataga cgatacagct agcacagccg gcttgcagct
1441 catcaatcag tacacatacc gtacgtgtct gaagtatgcc ttgttgatat gaacactgta
1501 ccgtggggcc agacgacacg gtctgaagca ccgcctctgc agcatctcgc acgattgatg
1561 cgcatcaatt gcttgcaatt cgatttgcat ttggcgcagt tgtctgcaca atctttgcca
1621 acgatcgcat gatccgattc aatgatccga agcatgatca atacaccgca ttagcagtgc
1681 atgcgcgatt gggacacctc tgggggggga tctgatgcac gttcgacacg ggtgccgaac
1741 ctgcgagaag gctcactaca cgcgtgcgga cgcatgcatg cagtgccaaa gatctcgttt
1801 gaactgcgcg ccagcacatt gcagtgcacg ccccgtgcca ggcgaaaacg tatttaaccc
1861 cgaagccagc atactttctc taccagttca tctcttgttg atctacctcc accacagtct
1921 atcagtgatt aacaaacaat ggccaagtgc gcatcgcgtg tcaatgctgg cgctgctgac
1981 gtccgctgcc taaactgcat gaaagctcct gcagtcgcaa gtagtccttt taacttgttg
2041 tatcggaaca aatggttgta gtctgtgccg tgtgacgatc ttgagccgcc tggtcggcag
2101 gtccaatgtc ggcctggacc ctgcacaccc tgcccaccct gccgctgctc acccgttcac
2161 gaccccacag gtgctcgcag cagtaattgt cgatagatag caactgtgtt ctgactattc
2221 tttgaccttc cgcatttggg actgcggcgg cacacctact atccgcaatg cacaccctgg
2281 tgctggtact gcctgccttt ccgcgcgagc ccacaccatg caggcgcata gtaatggact
2341 gtgattgatt taggcaactg tatgaaacct cttcatcgtg ccttggttgc ttgcgatacc
2401 ctcacgtgag ttcatatgac caacgctggc acgacccctg caggatcacc agcgccgttc
2461 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
2521 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
2581 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
2641 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
2701 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
2761 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggacggc agcggcgtga
2821 agcagaccct gaacttcgac ctgctgaagc tggcgggcga cgtggagagc aacccgggcc
2881 ccggctccgt attcagtcga ctggtaccaa catcagctat ggtgtccaag ggcgaggagg
2941 acaacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggcagcgtga
3001 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
3061 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgagcc
3121 cccagttcat gtacggcagc aaggcctacg tgaagcaccc cgccgacatc cccgactacc
3181 tgaagctgag cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
3241 gcgtggtgac cgtgacccag gacagcagcc tccaggacgg cgagttcatc tacaaggtga
3301 agctgcgcgg caccaacttc cccagcgacg gccccgtgat gcagaagaag accatgggct
3361 gggaggccag cagcgagcgc atgtaccccg aggacggcgc cctgaagggc gagatcaagc
3421 agcgcctgaa gctgaaggac ggcggccact acgacgccga ggtgaagacc acctacaagg
3481 ccaagaagcc cgtgcagctg cccggcgcct acaacgtgaa catcaagctg gacatcacca
3541 gccacaacga ggactacacc atcgtggagc agtacgagcg cgctgagggc cgccacagca
3601 ccggcggcat ggacgagctg tacaagtaac tgcgcagtca ggcagagtcg ttgttgggtg
3661 tatggttgca caaaactaga cctcgagctg gtcggttaga aagagatgct tgtgcgactg
3721 gatgtaacag gcacaatccg aggaaaatgc aacagaaacg ttatgatgta acatctgagc
3781 acagtcaata aaattgcatg ttgtatgccg cacacaaaag tgttgttaaa catgcgatgt
3841 ggttgtcaaa tcattattcg cacaaccatg gaccgaacct ctggcacgct gcatacgctg
3901 tattcattca catcatgttt tctacttgtc acatgctctg caccaagtaa ctccgattag
3961 ttaggttcgc acatgtcatc ggagtggacc acttcgaatc atttggttcg aagataaacc
4021 ggactataag ctaggtagta actagtgcga ctgtctagaa tcgcgtatgc gagtgtatga
4081 gctcgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
4141 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaataatat catcatgaac
4201 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
4261 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
4321 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
4381 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
4441 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
4501 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
4561 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
4621 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
4681 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
4741 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
4801 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
4861 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
4921 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
4981 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
5041 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
5101 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
5161 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
5221 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
5281 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
5341 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
5401 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
5461 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
5521 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
5581 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
5641 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
5701 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
5761 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
5821 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
5881 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
5941 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
6001 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
6061 tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa
6121 aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt
6181 cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt
6241 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagacttcc
6301 gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc
6361 atggatgttt tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggcccac
6421 ct
//