LOCUS pGG-pUM140_0016-BleR-E2A-mCherry-UmtRbcS 6416 bp DNA circular UNA 11-APR-2023 DEFINITION . ACCESSION urn.local.jonasblomme.p6-cgtpvik VERSION urn.local.jonasblomme.p6-cgtpvik KEYWORDS . SOURCE ORGANISM . COMMENT This file is created by Vector NTI http://www.invitrogen.com/. FEATURES Location/Qualifiers promoter 1..1934 /modified_by="jonasblomme" /label="pUM140_0016" CDS join(1939..1946,2444..2807) /gene="ble" /note="derived from Streptoalloteichus hindustanus, adapted to Chlamydomonas reinhardtii and Ulva mutabilis codon usage" /codon_start=1 /transl_table=11 /product="bleomycin resistance protein" /protein_id="ABY50194.1" /db_xref="GI:163962062" /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW GREFALRDPAGNCVHFVAEEQD" intron 1947..2443 /label="RbcsI" CDS 2808..2876 /label="E2A" Linker 2877..2906 /origin="C-linker-D (53bp linear)Fragment: from BsaI cut at 12^13 to BsaI cut at 40^41, 28bpOrientation: originalPlus strand 5': no changePlus strand 3': no changeMinus strand 5': no changeMinus strand 3': no change" CDS 2913..3620 /SerialCloner_Color="&hFF0000" /SerialCloner_Show="True" /SerialCloner_Protect="True" /SerialCloner_Arrow="True" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="mCherry" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; mCherry" /modified_by="jonasblomme" /label="mCherry" terminator 3628..4016 /regulatory_class="terminator" /note="Ulva RbcS terminator" /label="UmtRbcS" Linker 4021..4069 /created_by="wacae" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker II" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II" /label="Linker II" primer complement(4106..4126) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 4187..4237 /created_by="chgai" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 4238..5047 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" rep_origin 5194..5782 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 6381..6401 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" ORIGIN 1 tgccgaattc gcagtgaacc cgcatggcag ggtggtcaca ggtgtccttt ggctgcacag 61 taacatgacc gtcatgcatg ggacagaggt tgtgagcagc atgcgtcgat gcatacatgc 121 gcatgtagca tgcagcagtg agctccagag tggaaagctg gcccgctgtc gtgtgtccgg 181 accgacaggc caggctctgg actgggccat agtcagcagg gcagaggtac tatgtctagc 241 gccttcctgg gtgtgcagct ttgccttgcc atgcttggac atgaatgagt ggggcgccag 301 gcacttacaa gagccacctc gtggtgtggg catatggcag aggggcaccc atcagtcgtg 361 tgccattcag accgtgacga agcaatcaat gactgtgctt gtccgggaga aagtctgttg 421 tgaaatttgc tgtgatctag gggccaatgt ggcagtcagg aatggctaga gatgctgcta 481 gtgtcgtgca tacctggtgt actgccaaca gcctcacctg accatggtgc ttgtgtttaa 541 agcaagcatg ccacaagtcg gcatgtaggc atacggcaag agctacacag ttggctgttg 601 acctgtggat gcgtgaaaca gaagtttgaa tcgtgccctt tcgcgttgat gcaccctgtg 661 tgggagccag gctggtgtgt gtggaagcca ggctggtggc acacctgtgt gacctggagg 721 tggacgaccg ggtggcgagg caagtagcag tttcatcaaa gaaacacaag ctgatgcatc 781 cgcgatagta gcacgattct aatgtgtgca cgcaaacgca tcggcccaac tggagcaggg 841 gatgcgcagc agcggccgct ggttccacga actcgaagcg caaagagcag tccgctgtga 901 ccagcattcc gctagtgtca gggacgatat gaatacgggt tgggcacatt atgtttgggt 961 cgaggtcgcc atggcggacg tgcaagcggc tgtcgatcac gcctatcgga gatgcccagc 1021 gcctgcatcg atatgcaacc agctctgcac cagcgcagca atacgacagc tgcagccgat 1081 ggcctcctag acactgtgca acaacaaaaa acaattggac cgtgacggca caggtgtcga 1141 acacaggggc tgagagagga ctgcctgtgc ttgtgctcca gtgctcatac gccatactga 1201 tacgcctcca ccgatggcct cactgcctgc ggacactggc agacgctgtc gtttggatgt 1261 cgcatcgcca tcttcagtga tgttgtggcg ttcggcagga tgtacgcatg agcgattctt 1321 ctaaacgagc agtggtggca catcagcggc tccctcaaac cacaatgccg gcttggaagg 1381 tagagcatct gctcggcaag ccgacataga cgatacagct agcacagccg gcttgcagct 1441 catcaatcag tacacatacc gtacgtgtct gaagtatgcc ttgttgatat gaacactgta 1501 ccgtggggcc agacgacacg gtctgaagca ccgcctctgc agcatctcgc acgattgatg 1561 cgcatcaatt gcttgcaatt cgatttgcat ttggcgcagt tgtctgcaca atctttgcca 1621 acgatcgcat gatccgattc aatgatccga agcatgatca atacaccgca ttagcagtgc 1681 atgcgcgatt gggacacctc tgggggggga tctgatgcac gttcgacacg ggtgccgaac 1741 ctgcgagaag gctcactaca cgcgtgcgga cgcatgcatg cagtgccaaa gatctcgttt 1801 gaactgcgcg ccagcacatt gcagtgcacg ccccgtgcca ggcgaaaacg tatttaaccc 1861 cgaagccagc atactttctc taccagttca tctcttgttg atctacctcc accacagtct 1921 atcagtgatt aacaaacaat ggccaagtgc gcatcgcgtg tcaatgctgg cgctgctgac 1981 gtccgctgcc taaactgcat gaaagctcct gcagtcgcaa gtagtccttt taacttgttg 2041 tatcggaaca aatggttgta gtctgtgccg tgtgacgatc ttgagccgcc tggtcggcag 2101 gtccaatgtc ggcctggacc ctgcacaccc tgcccaccct gccgctgctc acccgttcac 2161 gaccccacag gtgctcgcag cagtaattgt cgatagatag caactgtgtt ctgactattc 2221 tttgaccttc cgcatttggg actgcggcgg cacacctact atccgcaatg cacaccctgg 2281 tgctggtact gcctgccttt ccgcgcgagc ccacaccatg caggcgcata gtaatggact 2341 gtgattgatt taggcaactg tatgaaacct cttcatcgtg ccttggttgc ttgcgatacc 2401 ctcacgtgag ttcatatgac caacgctggc acgacccctg caggatcacc agcgccgttc 2461 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt 2521 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt 2581 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc 2641 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg 2701 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc 2761 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggacggc agcggccagt 2821 gcacgaacta cgccctgctg aagctggcgg gcgacgtgga gagcaacccg ggcccgggct 2881 ccgtattcag tcgactggta ccaacatcag ctatggtgtc caagggcgag gaggacaaca 2941 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggcagc gtgaacggcc 3001 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca 3061 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg agcccccagt 3121 tcatgtacgg cagcaaggcc tacgtgaagc accccgccga catccccgac tacctgaagc 3181 tgagcttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg 3241 tgaccgtgac ccaggacagc agcctccagg acggcgagtt catctacaag gtgaagctgc 3301 gcggcaccaa cttccccagc gacggccccg tgatgcagaa gaagaccatg ggctgggagg 3361 ccagcagcga gcgcatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagcgcc 3421 tgaagctgaa ggacggcggc cactacgacg ccgaggtgaa gaccacctac aaggccaaga 3481 agcccgtgca gctgcccggc gcctacaacg tgaacatcaa gctggacatc accagccaca 3541 acgaggacta caccatcgtg gagcagtacg agcgcgctga gggccgccac agcaccggcg 3601 gcatggacga gctgtacaag taactgcgca gtcaggcaga gtcgttgttg ggtgtatggt 3661 tgcacaaaac tagacctcga gctggtcggt tagaaagaga tgcttgtgcg actggatgta 3721 acaggcacaa tccgaggaaa atgcaacaga aacgttatga tgtaacatct gagcacagtc 3781 aataaaattg catgttgtat gccgcacaca aaagtgttgt taaacatgcg atgtggttgt 3841 caaatcatta ttcgcacaac catggaccga acctctggca cgctgcatac gctgtattca 3901 ttcacatcat gttttctact tgtcacatgc tctgcaccaa gtaactccga ttagttaggt 3961 tcgcacatgt catcggagtg gaccacttcg aatcatttgg ttcgaagata aaccggacta 4021 taagctaggt agtaactagt gcgactgtct agaatcgcgt atgcgagtgt atgagctcga 4081 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt 4141 gtctcaaaat ctctgatgtt acattgcaca agataaaata atatcatcat gaacaataaa 4201 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac 4261 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg 4321 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc 4381 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt 4441 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac 4501 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt 4561 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg 4621 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc 4681 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg 4741 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc 4801 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa 4861 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc 4921 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa 4981 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt 5041 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg 5101 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc 5161 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 5221 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 5281 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 5341 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 5401 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 5461 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 5521 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 5581 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 5641 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 5701 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 5761 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 5821 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 5881 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 5941 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 6001 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 6061 caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca 6121 tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc 6181 cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta 6241 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagac ttccgactga 6301 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat 6361 gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc ccacct //