LOCUS pGG-pUM140_0016-BleR-E2A-mCherry-UmtRbcS 6416 bp DNA circular UNA 11-APR-2023
DEFINITION .
ACCESSION urn.local.jonasblomme.p6-cgtpvik
VERSION urn.local.jonasblomme.p6-cgtpvik
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/.
FEATURES Location/Qualifiers
promoter 1..1934
/modified_by="jonasblomme"
/label="pUM140_0016"
CDS join(1939..1946,2444..2807)
/gene="ble"
/note="derived from Streptoalloteichus hindustanus,
adapted to Chlamydomonas reinhardtii and Ulva mutabilis
codon usage"
/codon_start=1
/transl_table=11
/product="bleomycin resistance protein"
/protein_id="ABY50194.1"
/db_xref="GI:163962062"
/translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
GREFALRDPAGNCVHFVAEEQD"
intron 1947..2443
/label="RbcsI"
CDS 2808..2876
/label="E2A"
Linker 2877..2906
/origin="C-linker-D (53bp linear)Fragment: from BsaI cut
at 12^13 to BsaI cut at 40^41, 28bpOrientation:
originalPlus strand 5': no changePlus strand 3': no
changeMinus strand 5': no changeMinus strand 3': no
change"
CDS 2913..3620
/SerialCloner_Color="&hFF0000"
/SerialCloner_Show="True"
/SerialCloner_Protect="True"
/SerialCloner_Arrow="True"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="mCherry"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; mCherry"
/modified_by="jonasblomme"
/label="mCherry"
terminator 3628..4016
/regulatory_class="terminator"
/note="Ulva RbcS terminator"
/label="UmtRbcS"
Linker 4021..4069
/created_by="wacae"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Linker II"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II"
/label="Linker II"
primer complement(4106..4126)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13R"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
(pCDB-Cas9 - GWM13R)"
/label="GWM13R"
promoter 4187..4237
/created_by="chgai"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 4238..5047
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
rep_origin 5194..5782
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 6381..6401
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13F"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
(pCDB-Cas9 - GWM13F)"
/label="GWM13F"
ORIGIN
1 tgccgaattc gcagtgaacc cgcatggcag ggtggtcaca ggtgtccttt ggctgcacag
61 taacatgacc gtcatgcatg ggacagaggt tgtgagcagc atgcgtcgat gcatacatgc
121 gcatgtagca tgcagcagtg agctccagag tggaaagctg gcccgctgtc gtgtgtccgg
181 accgacaggc caggctctgg actgggccat agtcagcagg gcagaggtac tatgtctagc
241 gccttcctgg gtgtgcagct ttgccttgcc atgcttggac atgaatgagt ggggcgccag
301 gcacttacaa gagccacctc gtggtgtggg catatggcag aggggcaccc atcagtcgtg
361 tgccattcag accgtgacga agcaatcaat gactgtgctt gtccgggaga aagtctgttg
421 tgaaatttgc tgtgatctag gggccaatgt ggcagtcagg aatggctaga gatgctgcta
481 gtgtcgtgca tacctggtgt actgccaaca gcctcacctg accatggtgc ttgtgtttaa
541 agcaagcatg ccacaagtcg gcatgtaggc atacggcaag agctacacag ttggctgttg
601 acctgtggat gcgtgaaaca gaagtttgaa tcgtgccctt tcgcgttgat gcaccctgtg
661 tgggagccag gctggtgtgt gtggaagcca ggctggtggc acacctgtgt gacctggagg
721 tggacgaccg ggtggcgagg caagtagcag tttcatcaaa gaaacacaag ctgatgcatc
781 cgcgatagta gcacgattct aatgtgtgca cgcaaacgca tcggcccaac tggagcaggg
841 gatgcgcagc agcggccgct ggttccacga actcgaagcg caaagagcag tccgctgtga
901 ccagcattcc gctagtgtca gggacgatat gaatacgggt tgggcacatt atgtttgggt
961 cgaggtcgcc atggcggacg tgcaagcggc tgtcgatcac gcctatcgga gatgcccagc
1021 gcctgcatcg atatgcaacc agctctgcac cagcgcagca atacgacagc tgcagccgat
1081 ggcctcctag acactgtgca acaacaaaaa acaattggac cgtgacggca caggtgtcga
1141 acacaggggc tgagagagga ctgcctgtgc ttgtgctcca gtgctcatac gccatactga
1201 tacgcctcca ccgatggcct cactgcctgc ggacactggc agacgctgtc gtttggatgt
1261 cgcatcgcca tcttcagtga tgttgtggcg ttcggcagga tgtacgcatg agcgattctt
1321 ctaaacgagc agtggtggca catcagcggc tccctcaaac cacaatgccg gcttggaagg
1381 tagagcatct gctcggcaag ccgacataga cgatacagct agcacagccg gcttgcagct
1441 catcaatcag tacacatacc gtacgtgtct gaagtatgcc ttgttgatat gaacactgta
1501 ccgtggggcc agacgacacg gtctgaagca ccgcctctgc agcatctcgc acgattgatg
1561 cgcatcaatt gcttgcaatt cgatttgcat ttggcgcagt tgtctgcaca atctttgcca
1621 acgatcgcat gatccgattc aatgatccga agcatgatca atacaccgca ttagcagtgc
1681 atgcgcgatt gggacacctc tgggggggga tctgatgcac gttcgacacg ggtgccgaac
1741 ctgcgagaag gctcactaca cgcgtgcgga cgcatgcatg cagtgccaaa gatctcgttt
1801 gaactgcgcg ccagcacatt gcagtgcacg ccccgtgcca ggcgaaaacg tatttaaccc
1861 cgaagccagc atactttctc taccagttca tctcttgttg atctacctcc accacagtct
1921 atcagtgatt aacaaacaat ggccaagtgc gcatcgcgtg tcaatgctgg cgctgctgac
1981 gtccgctgcc taaactgcat gaaagctcct gcagtcgcaa gtagtccttt taacttgttg
2041 tatcggaaca aatggttgta gtctgtgccg tgtgacgatc ttgagccgcc tggtcggcag
2101 gtccaatgtc ggcctggacc ctgcacaccc tgcccaccct gccgctgctc acccgttcac
2161 gaccccacag gtgctcgcag cagtaattgt cgatagatag caactgtgtt ctgactattc
2221 tttgaccttc cgcatttggg actgcggcgg cacacctact atccgcaatg cacaccctgg
2281 tgctggtact gcctgccttt ccgcgcgagc ccacaccatg caggcgcata gtaatggact
2341 gtgattgatt taggcaactg tatgaaacct cttcatcgtg ccttggttgc ttgcgatacc
2401 ctcacgtgag ttcatatgac caacgctggc acgacccctg caggatcacc agcgccgttc
2461 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
2521 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
2581 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
2641 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
2701 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
2761 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggacggc agcggccagt
2821 gcacgaacta cgccctgctg aagctggcgg gcgacgtgga gagcaacccg ggcccgggct
2881 ccgtattcag tcgactggta ccaacatcag ctatggtgtc caagggcgag gaggacaaca
2941 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggcagc gtgaacggcc
3001 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
3061 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg agcccccagt
3121 tcatgtacgg cagcaaggcc tacgtgaagc accccgccga catccccgac tacctgaagc
3181 tgagcttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
3241 tgaccgtgac ccaggacagc agcctccagg acggcgagtt catctacaag gtgaagctgc
3301 gcggcaccaa cttccccagc gacggccccg tgatgcagaa gaagaccatg ggctgggagg
3361 ccagcagcga gcgcatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagcgcc
3421 tgaagctgaa ggacggcggc cactacgacg ccgaggtgaa gaccacctac aaggccaaga
3481 agcccgtgca gctgcccggc gcctacaacg tgaacatcaa gctggacatc accagccaca
3541 acgaggacta caccatcgtg gagcagtacg agcgcgctga gggccgccac agcaccggcg
3601 gcatggacga gctgtacaag taactgcgca gtcaggcaga gtcgttgttg ggtgtatggt
3661 tgcacaaaac tagacctcga gctggtcggt tagaaagaga tgcttgtgcg actggatgta
3721 acaggcacaa tccgaggaaa atgcaacaga aacgttatga tgtaacatct gagcacagtc
3781 aataaaattg catgttgtat gccgcacaca aaagtgttgt taaacatgcg atgtggttgt
3841 caaatcatta ttcgcacaac catggaccga acctctggca cgctgcatac gctgtattca
3901 ttcacatcat gttttctact tgtcacatgc tctgcaccaa gtaactccga ttagttaggt
3961 tcgcacatgt catcggagtg gaccacttcg aatcatttgg ttcgaagata aaccggacta
4021 taagctaggt agtaactagt gcgactgtct agaatcgcgt atgcgagtgt atgagctcga
4081 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
4141 gtctcaaaat ctctgatgtt acattgcaca agataaaata atatcatcat gaacaataaa
4201 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
4261 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
4321 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc
4381 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
4441 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
4501 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt
4561 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
4621 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
4681 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
4741 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc
4801 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
4861 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
4921 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
4981 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
5041 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg
5101 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
5161 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
5221 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
5281 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
5341 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
5401 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
5461 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
5521 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
5581 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
5641 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
5701 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
5761 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
5821 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
5881 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
5941 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
6001 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
6061 caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca
6121 tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc
6181 cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta
6241 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagac ttccgactga
6301 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat
6361 gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc ccacct
//