LOCUS pGG-UmpRbcS-BleR-T2A-YFP-UmtRbcS 5031 bp DNA circular UNA 11-APR-2023
DEFINITION .
ACCESSION urn.local.jonasblomme.p5-cgtpvik
VERSION urn.local.jonasblomme.p5-cgtpvik
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/.
FEATURES Location/Qualifiers
promoter 1..546
/regulatory_class="promoter"
/note="Ulva RbcS promoter"
/label="UmpRbcS"
CDS join(551..558,1056..1419)
/gene="ble"
/note="derived from Streptoalloteichus hindustanus,
adapted to Chlamydomonas reinhardtii and Ulva mutabilis
codon usage"
/codon_start=1
/transl_table=11
/product="bleomycin resistance protein"
/protein_id="ABY50194.1"
/db_xref="GI:163962062"
/translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
GREFALRDPAGNCVHFVAEEQD"
intron 559..1055
/label="RbcsI"
CDS 1420..1482
/modified_by="jonasblomme"
/label="T2A"
Linker 1483..1512
/origin="C-linker-D (53bp linear)Fragment: from BsaI cut
at 12^13 to BsaI cut at 40^41, 28bpOrientation:
originalPlus strand 5': no changePlus strand 3': no
changeMinus strand 5': no changeMinus strand 3': no
change"
CDS 1519..2238
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
/modified_by="jonasblomme"
/label="YFP"
terminator 2243..2631
/regulatory_class="terminator"
/note="Ulva RbcS terminator"
/modified_by="jonasblomme"
/label="UmtRbcS"
Linker 2636..2684
/created_by="wacae"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Linker II"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II"
/label="Linker II"
primer complement(2721..2741)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13R"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
(pCDB-Cas9 - GWM13R)"
/label="GWM13R"
promoter 2802..2852
/created_by="chgai"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 2853..3662
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
rep_origin 3809..4397
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 4996..5016
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13F"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
(pCDB-Cas9 - GWM13F)"
/label="GWM13F"
ORIGIN
1 cgttgttcgt acacaaggat gtcgcttgtg gctttttcgc tcggctttcc aggagggcag
61 tccagatacc atgcggccgc cgtgtccatc actcgcgctg cagctgatgt gggcagatac
121 tgcactaatc tgccgcacga cgcgtcggcc tgagtcggcg gacaccggag gcttgctgga
181 gtactggcac aagtgtgcgt gtgattgcca gaaacagcaa cccactcact agatataccg
241 ctccaataat gggttcatgt gcaagctcac tcaataacga aacgcttcac tattgtgcac
301 gtgccggggt tgcttgggta tggcgtgcca tacgggttct gcacatgtca cccacgtggg
361 tgtggcacga gatatcgagg tggccatgta aaaggcattt gctagagggc agctcgtcca
421 tgccgctcga gcacaccgag tgcgcaccat aaatgcagca catgcctgtg cagcctatct
481 cctgtctctg ttacatccca ccacctcctc acatatcccc ttccaccctt aaaccgcaca
541 gcagacaaca atggccaagt gcgcatcgcg tgtcaatgct ggcgctgctg acgtccgctg
601 cctaaactgc atgaaagctc ctgcagtcgc aagtagtcct tttaacttgt tgtatcggaa
661 caaatggttg tagtctgtgc cgtgtgacga tcttgagccg cctggtcggc aggtccaatg
721 tcggcctgga ccctgcacac cctgcccacc ctgccgctgc tcacccgttc acgaccccac
781 aggtgctcgc agcagtaatt gtcgatagat agcaactgtg ttctgactat tctttgacct
841 tccgcatttg ggactgcggc ggcacaccta ctatccgcaa tgcacaccct ggtgctggta
901 ctgcctgcct ttccgcgcga gcccacacca tgcaggcgca tagtaatgga ctgtgattga
961 tttaggcaac tgtatgaaac ctcttcatcg tgccttggtt gcttgcgata ccctcacgtg
1021 agttcatatg accaacgctg gcacgacccc tgcaggatca ccagcgccgt tccggtgctc
1081 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
1141 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
1201 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
1261 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
1321 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
1381 gccggcaact gcgtgcactt cgtggccgag gagcaggacg gcagcggcga gggcagagga
1441 agtcttctaa catgcggtga cgtggaggag aatccgggcc ctggctccgt attcagtcga
1501 ctggtaccaa catcagctat ggtgagcaag ggcgaggagc tgttcacggg cgtggtgccc
1561 atcctggtgg agctggacgg cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc
1621 gagggcgacg ccacctacgg caagctgacc ctgaagttca tctgcacgac cggcaagctg
1681 cccgtgccct ggcccaccct ggtgaccacc ttcggctacg gcctgcagtg cttcgcccgc
1741 taccccgacc acatgaagca gcacgacttc ttcaagagcg ccatgccgga gggctacgtg
1801 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc ggaggtgaag
1861 ttcgagggcg acacgctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
1921 ggcaacatcc tgggccacaa gctggagtac aactacaaca gccacaacgt gtacatcatg
1981 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
2041 ggctcggtgc agctggcgga ccactaccag cagaacaccc ccatcggcga cggcccggtg
2101 ctgctgcccg acaaccacta cctgtcctac cagagcgccc tgtccaagga ccccaacgag
2161 aagcgcgacc acatggtgct gctggagttc gtgaccgccg ccggcatcac cctgggcatg
2221 gacgagctgt acaagtgact gcgcagtcag gcagagtcgt tgttgggtgt atggttgcac
2281 aaaactagac ctcgagctgg tcggttagaa agagatgctt gtgcgactgg atgtaacagg
2341 cacaatccga ggaaaatgca acagaaacgt tatgatgtaa catctgagca cagtcaataa
2401 aattgcatgt tgtatgccgc acacaaaagt gttgttaaac atgcgatgtg gttgtcaaat
2461 cattattcgc acaaccatgg accgaacctc tggcacgctg catacgctgt attcattcac
2521 atcatgtttt ctacttgtca catgctctgc accaagtaac tccgattagt taggttcgca
2581 catgtcatcg gagtggacca cttcgaatca tttggttcga agataaaccg gactataagc
2641 taggtagtaa ctagtgcgac tgtctagaat cgcgtatgcg agtgtatgag ctcgatatcc
2701 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
2761 aaaatctctg atgttacatt gcacaagata aaataatatc atcatgaaca ataaaactgt
2821 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
2881 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
2941 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
3001 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
3061 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
3121 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag
3181 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
3241 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
3301 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
3361 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
3421 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
3481 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
3541 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
3601 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
3661 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
3721 gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
3781 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
3841 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
3901 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
3961 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
4021 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
4081 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
4141 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
4201 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
4261 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
4321 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
4381 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
4441 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
4501 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
4561 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
4621 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
4681 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
4741 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
4801 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
4861 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagacttccg actgagcctt
4921 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt
4981 cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggcccacc t
//