LOCUS pGG-UmpRbcS-BleR-E2A-B-ccdB-CmR-G-UmtRbcS 5733 bp DNA circular UNA 11-APR-2023
DEFINITION .
ACCESSION urn.local.jonasblomme.p-cn2ko84
VERSION urn.local.jonasblomme.p-cn2ko84
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/.
FEATURES Location/Qualifiers
promoter 1..546
/regulatory_class="promoter"
/note="Ulva RbcS promoter"
/label="UmpRbcS"
CDS join(551..558,1056..1419)
/gene="ble"
/note="derived from Streptoalloteichus hindustanus,
adapted to Chlamydomonas reinhardtii and Ulva mutabilis
codon usage"
/codon_start=1
/transl_table=11
/product="bleomycin resistance protein"
/protein_id="ABY50194.1"
/db_xref="GI:163962062"
/translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
GREFALRDPAGNCVHFVAEEQD"
intron 559..1055
/label="RbcsI"
CDS 1420..1488
/label="E2A"
GG complement(1494..1504)
/created_by="thjac"
/modified_by="thjac"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="B"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; B"
/label="B"
CDS complement(1511..1816)
/vntifkey=4
/label="ccdB"
UTR complement(1817..2135)
/Mismatches=1
/%_Identity="99.68652037617555"
/Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="99.37%"
/Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB
5'UTR (ccdB)"
/label="ccdB 5'UTR"
misc_feature complement(2158..2817)
/Transferred_From="cmR"
/Transferred_Similarity="99.85%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="14 CmR"
/Transferred_Similarity="99.85%"
/Primary_Match="99.85%; Adjusted-Interval-0:0/0; CmR (14 CmR)"
/Alternative_Match="99.85%; Adjusted-Interval-0:0/0; CmR"
/label="CmR"
/note="Geneious type: Bacterial Selection"
promoter complement(2871..2901)
/note="Lac promoter of Escherichia coli containing 2 base
pair mutations"
/regulatory_class="promoter"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="lacUV5"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="14 CmR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
(14 CmR)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
lacUV5 (CmR)"
/label="lacUV5"
GG 2926..2936
/%_Identity=100
/Motif="GGTCTCAGTAT"
/modified_by="thjac"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="G"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; G
overlap (G)"
/modified_by="mamee"
/label="G"
terminator 2945..3333
/regulatory_class="terminator"
/note="Ulva RbcS terminator"
/modified_by="jonasblomme"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="UmtRbcS"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; UmtRbcS"
/label="UmtRbcS"
Linker 3338..3386
/created_by="wacae"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Linker II"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II"
/label="Linker II"
primer complement(3423..3443)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13R"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
(pCDB-Cas9 - GWM13R)"
/label="GWM13R"
promoter 3504..3554
/created_by="chgai"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 3555..4364
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
rep_origin 4511..5099
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 5698..5718
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13F"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
(pCDB-Cas9 - GWM13F)"
/label="GWM13F"
ORIGIN
1 cgttgttcgt acacaaggat gtcgcttgtg gctttttcgc tcggctttcc aggagggcag
61 tccagatacc atgcggccgc cgtgtccatc actcgcgctg cagctgatgt gggcagatac
121 tgcactaatc tgccgcacga cgcgtcggcc tgagtcggcg gacaccggag gcttgctgga
181 gtactggcac aagtgtgcgt gtgattgcca gaaacagcaa cccactcact agatataccg
241 ctccaataat gggttcatgt gcaagctcac tcaataacga aacgcttcac tattgtgcac
301 gtgccggggt tgcttgggta tggcgtgcca tacgggttct gcacatgtca cccacgtggg
361 tgtggcacga gatatcgagg tggccatgta aaaggcattt gctagagggc agctcgtcca
421 tgccgctcga gcacaccgag tgcgcaccat aaatgcagca catgcctgtg cagcctatct
481 cctgtctctg ttacatccca ccacctcctc acatatcccc ttccaccctt aaaccgcaca
541 gcagacaaca atggccaagt gcgcatcgcg tgtcaatgct ggcgctgctg acgtccgctg
601 cctaaactgc atgaaagctc ctgcagtcgc aagtagtcct tttaacttgt tgtatcggaa
661 caaatggttg tagtctgtgc cgtgtgacga tcttgagccg cctggtcggc aggtccaatg
721 tcggcctgga ccctgcacac cctgcccacc ctgccgctgc tcacccgttc acgaccccac
781 aggtgctcgc agcagtaatt gtcgatagat agcaactgtg ttctgactat tctttgacct
841 tccgcatttg ggactgcggc ggcacaccta ctatccgcaa tgcacaccct ggtgctggta
901 ctgcctgcct ttccgcgcga gcccacacca tgcaggcgca tagtaatgga ctgtgattga
961 tttaggcaac tgtatgaaac ctcttcatcg tgccttggtt gcttgcgata ccctcacgtg
1021 agttcatatg accaacgctg gcacgacccc tgcaggatca ccagcgccgt tccggtgctc
1081 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
1141 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
1201 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
1261 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
1321 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
1381 gccggcaact gcgtgcactt cgtggccgag gagcaggacg gcagcggcca gtgcacgaac
1441 tacgccctgc tgaagctggc gggcgacgtg gagagcaacc cgggcccggg ctcaacatga
1501 gaccgtcgac ttatattccc cagaacatca ggttaatggc gtttttgatg tcattttcgc
1561 ggtggctgag atcagccact tcttccccga taacggagac tggcacactg gccatatcgg
1621 tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacgggaga
1681 ctttatctga cagcagacgt gcactggcca gggggatcac catccgtcgc ccgggcgtgt
1741 caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt
1801 aaaccttaaa ctgcatttca ccagtccctg ttctcgtcag caaaagagcc gttcatttca
1861 ataaaccggg cgacctcagc catcccttcc tgattttccg ctttccagcg ttcggcacgc
1921 agacgacggg cttcattctg catggttgtg cttaccagac cggagatatt gacatcatat
1981 atgccttgag caactgatag ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag
2041 cacacctctt tttgacatac ttcgggtata catatcagta tatattctta taccgcaaaa
2101 atcagcgcgc aaatacgcat actgttatct ggcttttagt aagccggatc cacgcgttta
2161 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
2221 gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
2281 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
2341 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
2401 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
2461 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
2521 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
2581 gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa gaatgtgaat
2641 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
2701 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
2761 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
2821 agcttcctta gctcctgaaa atctcgccgg atcctaactc aaaatccaca cattatacga
2881 gccggaagca taaagtgtaa agcctggggt gcctaatgcg gccgcggtct cagtatctga
2941 ctgcgcagtc aggcagagtc gttgttgggt gtatggttgc acaaaactag acctcgagct
3001 ggtcggttag aaagagatgc ttgtgcgact ggatgtaaca ggcacaatcc gaggaaaatg
3061 caacagaaac gttatgatgt aacatctgag cacagtcaat aaaattgcat gttgtatgcc
3121 gcacacaaaa gtgttgttaa acatgcgatg tggttgtcaa atcattattc gcacaaccat
3181 ggaccgaacc tctggcacgc tgcatacgct gtattcattc acatcatgtt ttctacttgt
3241 cacatgctct gcaccaagta actccgatta gttaggttcg cacatgtcat cggagtggac
3301 cacttcgaat catttggttc gaagataaac cggactataa gctaggtagt aactagtgcg
3361 actgtctaga atcgcgtatg cgagtgtatg agctcgatat cccctatagt gagtcgtatt
3421 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
3481 ttgcacaaga taaaataata tcatcatgaa caataaaact gtctgcttac ataaacagta
3541 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
3601 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
3661 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
3721 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
3781 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
3841 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
3901 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
3961 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
4021 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
4081 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
4141 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
4201 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
4261 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
4321 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
4381 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
4441 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
4501 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
4561 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
4621 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
4681 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
4741 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
4801 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
4861 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
4921 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
4981 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
5041 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
5101 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
5161 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
5221 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
5281 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
5341 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
5401 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
5461 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
5521 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
5581 acaacagata aaacgaaagg cccagacttc cgactgagcc tttcgtttta tttgatgcct
5641 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
5701 aaacgacggc cagtcttaag ctcgggccca cct
//