LOCUS       pGG-AG-PIBRT-KmR        6318 bp    DNA     circular UNA 11-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.7o-cn3yuqe
VERSION     urn.local.jonasblomme.7o-cn3yuqe
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     GG              complement(1..11)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2b8-ciz7x4i"">A</a>"
                     /label="A"
     CDS             complement(18..323)
                     /vntifkey=4
                     /label="ccdB"
     UTR             complement(324..642)
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.37%"
                     /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:thjac:t-cj5879l"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    complement(665..1324)
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="14 CmR"
                     /Transferred_Similarity="99.85%"
                     /Primary_Match="99.85%; Adjusted-Interval-0:0/0; CmR (<a
                     href=""urn:local:.:1h-fz09kes"">14 CmR</a>)"
                     /Alternative_Match="99.85%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     promoter        complement(1378..1408)
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="14 CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:1h-fz09kes"">14 CmR</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacUV5 (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     GG              1433..1443
                     /%_Identity=100
                     /Motif="GGTCTCAGTAT"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="G"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; G
                     overlap (<a href=""urn:local:thjac:2bm-ciz835c"">G</a>)"
                     /modified_by="mamee"
                     /label="G"
     promoter        1453..1998
                     /regulatory_class="promoter"
                     /note="Ulva mutabilis RbcS promoter"
                     /label="UmpRbcS"
     CDS             join(1999..2006,2504..2867)
                     /gene="ble"
                     /note="derived from Streptoalloteichus hindustanus,
                     adapted to Chlamydomonas reinhardtii and Ulva mutabilis
                     codon usage"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="ABY50194.1"
                     /db_xref="GI:163962062"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD"
     intron          2007..2503
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
                     /label="RbcsI"
     CDS             2868..3578
                     /label="mCherry"
     terminator      3579..3987
                     /modified_by="jonasblomme"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BleR"
                     /Transferred_Similarity="99.76%"
                     /Primary_Match="99.76%; Adjusted-Interval-0:0/0; Ulva
                     trbcS (<a
                     href=""urn:local:jonasblomme:1y-cnsq4yk"">BleR</a>)"
                     /Alternative_Match="99.49%; Adjusted-Interval-0:0/-20; <a
                     href=""urn:local:jonasblomme:w-cnsppqr"">UmtRbcS</a>"
                     /label="Ulva trbcS"
     primer          complement(4012..4032)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        4093..4143
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    4144..4953
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      5100..5688
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          6287..6307
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 accttgagac cgtcgactta tattccccag aacatcaggt taatggcgtt tttgatgtca
       61 ttttcgcggt ggctgagatc agccacttct tccccgataa cggagactgg cacactggcc
      121 atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca
      181 cgggagactt tatctgacag cagacgtgca ctggccaggg ggatcaccat ccgtcgcccg
      241 ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct ctctctttta
      301 taggtgtaaa ccttaaactg catttcacca gtccctgttc tcgtcagcaa aagagccgtt
      361 catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt tccagcgttc
      421 ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg agatattgac
      481 atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc
      541 ttcatagcac acctcttttt gacatacttc gggtatacat atcagtatat attcttatac
      601 cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc ttttagtaag ccggatccac
      661 gcgtttacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
      721 cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct
      781 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
      841 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
      901 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
      961 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     1021 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     1081 gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa
     1141 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     1201 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     1261 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     1321 ccattttagc ttccttagct cctgaaaatc tcgccggatc ctaactcaaa atccacacat
     1381 tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgcggcc gcggtctcag
     1441 tatgagctcg atcgttgttc gtacacaagg atgtcgcttg tggctttttc gctcggcttt
     1501 ccaggagggc agtccagata ccatgcggcc gccgtgtcca tcactcgcgc tgcagctgat
     1561 gtgggcagat actgcactaa tctgccgcac gacgcgtcgg cctgagtcgg cggacaccgg
     1621 aggcttgctg gagtactggc acaagtgtgc gtgtgattgc cagaaacagc aacccactca
     1681 ctagatatac cgctccaata atgggttcat gtgcaagctc actcaataac gaaacgcttc
     1741 actattgtgc acgtgccggg gttgcttggg tatggcgtgc catacgggtt ctgcacatgt
     1801 cacccacgtg ggtgtggcac gagatatcga ggtggccatg taaaaggcat ttgctagagg
     1861 gcagctcgtc catgccgctc gagcacaccg agtgcgcacc ataaatgcag cacatgcctg
     1921 tgcagcctat ctcctgtctc tgttacatcc caccacctcc tcacatatcc ccttccaccc
     1981 ttaaaccgca cagcagacat ggccaagtgc gcatcgcgtg tcaatgctgg cgctgctgac
     2041 gtccgctgcc taaactgcat gaaagctcct gcagtcgcaa gtagtccttt taacttgttg
     2101 tatcggaaca aatggttgta gtctgtgccg tgtgacgatc ttgagccgcc tggtcggcag
     2161 gtccaatgtc ggcctggacc ctgcacaccc tgcccaccct gccgctgctc acccgttcac
     2221 gaccccacag gtgctcgcag cagtaattgt cgatagatag caactgtgtt ctgactattc
     2281 tttgaccttc cgcatttggg actgcggcgg cacacctact atccgcaatg cacaccctgg
     2341 tgctggtact gcctgccttt ccgcgcgagc ccacaccatg caggcgcata gtaatggact
     2401 gtgattgatt taggcaactg tatgaaacct cttcatcgtg ccttggttgc ttgcgatacc
     2461 ctcacgtgag ttcatatgac caacgctggc acgacccctg caggatcacc agcgccgttc
     2521 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     2581 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     2641 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     2701 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     2761 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     2821 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggacatg gtgtccaagg
     2881 gcgaggagga caacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     2941 gcagcgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     3001 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
     3061 tcctgagccc ccagttcatg tacggcagca aggcctacgt gaagcacccc gccgacatcc
     3121 ccgactacct gaagctgagc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     3181 aggacggcgg cgtggtgacc gtgacccagg acagcagcct ccaggacggc gagttcatct
     3241 acaaggtgaa gctgcgcggc accaacttcc ccagcgacgg ccccgtgatg cagaagaaga
     3301 ccatgggctg ggaggccagc agcgagcgca tgtaccccga ggacggcgcc ctgaagggcg
     3361 agatcaagca gcgcctgaag ctgaaggacg gcggccacta cgacgccgag gtgaagacca
     3421 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtgaac atcaagctgg
     3481 acatcaccag ccacaacgag gactacacca tcgtggagca gtacgagcgc gctgagggcc
     3541 gccacagcac cggcggcatg gacgagctgt acaagtaagc agtcaggcag agtcgttgtt
     3601 gggtgtatgg ttgcacaaag ctagacctcg agctggtcgg ttagaaagag atgcttgtgc
     3661 gactggatgt aacaggcaca atccgaggaa aatgcaacag aaacgttatg atgtaacatc
     3721 tgagcacagt caataaaatt gcatgttgta tgccgcacac aaaagtgttg ttaaacatgc
     3781 gatgtggttg tcaaatcatt attcgcacaa ccatggaccg aacctctggc acgctgcata
     3841 cgctgtattc attcacatca tgttttctac ttgtcacatg ctctgcacca agtaactccg
     3901 attagttagg ttcgcacatg tcatcggagt ggaccacttc gaatcatttg gttcgaagac
     3961 aaaccggatc ccctatagtg agtcgtaatc ccctatagtg agtcgtatta catggtcata
     4021 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     4081 aaaataatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     4141 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     4201 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     4261 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     4321 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     4381 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     4441 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     4501 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     4561 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     4621 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     4681 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     4741 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     4801 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     4861 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     4921 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     4981 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     5041 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5101 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5161 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5221 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5281 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5341 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5401 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5461 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5521 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5581 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5641 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5701 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5761 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5821 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     5881 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     5941 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     6001 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     6061 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     6121 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     6181 aacgaaaggc ccagacttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     6241 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     6301 agtcttaagc tcgggccc
//
