LOCUS       pGG-D-E2A-E             2757 bp    DNA     circular SYN 11-APR-2023
DEFINITION  .
ACCESSION   KF718965
VERSION     KF718965.1
KEYWORDS    .
SOURCE      Cloning vector pGGB000 (unknown)
  ORGANISM  Cloning vector pGGB000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2j-c4nnd55"">D</a>"
                     /label="D"
     CDS             14..82
                     /label="E2A"
     GG              complement(83..93)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:29v-ciz7957"">E</a>"
                     /label="E"
     primer          complement(113..133)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        613..717
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    718..1578
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1749..2337
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          2701..2722
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcatca gctggcagcg gccagtgcac gaactacgcc ctgctgaagc tggcgggcga
       61 cgtggagagc aacccgggcc cgctgctgag accgaattct cgccctatag tgagtcgtat
      121 tacctggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      181 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
      241 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
      301 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
      361 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
      421 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
      481 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
      541 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
      601 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      661 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      721 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
      781 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
      841 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
      901 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
      961 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1021 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1081 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1141 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1201 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1261 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1321 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     1381 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggctctcga
     1441 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     1501 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     1561 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     1621 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     1681 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1741 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1801 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1861 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1921 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1981 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2041 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2101 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2161 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2221 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2281 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2341 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2401 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     2461 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     2521 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     2581 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     2641 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     2701 gtgagcggat aacaatttca cacgagctat ttaggtgaca ctatagaagt gaagctt
//
