LOCUS       pGG-B-NLS-T2A-C         2773 bp ds-DNA     circular SYN 11-APR-2023
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   alsaf
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2019 from SnapGene 4.2.0 http://www.snapgene.com
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2h-c4nnd55"">B</a>"
                     /label="B"
     NLS             15..35
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     (<ahref=""urn:local:warddecaestecker:2t-c8811q6"">NLSSV40<
                     /a>)"
                     /label="SV40"
     CDS             45..98
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     GG              complement(99..109)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:294-ciz70s8"">C</a>"
                     /label="C"
     primer          complement(129..149)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        629..733
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    734..1594
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1765..2353
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          2717..2738
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac aatgcctaag aagaagagga aggttggaag cggagagggc agaggaagtc
       61 tgctaacatg cggtgacgtc gaggagaatc ctggcccagg cttgagaccg aattctcgcc
      121 ctatagtgag tcgtattacc tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      181 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      241 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
      301 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
      361 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
      421 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
      481 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
      541 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
      601 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      661 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      721 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      781 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      841 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
      901 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      961 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     1021 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1081 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     1141 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     1201 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     1261 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     1321 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     1381 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     1441 tgagcgtggc tctcgaggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     1501 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     1561 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     1621 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1681 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1741 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1801 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1861 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1921 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1981 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2041 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2101 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2161 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2221 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2281 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2341 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2401 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     2461 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     2521 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     2581 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     2641 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     2701 tatgttgtgt ggaattgtga gcggataaca atttcacacg agctatttag gtgacactat
     2761 agaagtgaag ctt
//
