LOCUS       pGG-B-NLS-P2A-C         2776 bp ds-DNA     circular SYN 11-APR-2023
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   alsaf
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2019 from SnapGene 4.2.0 http://www.snapgene.com
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2h-c4nnd55"">B</a>"
                     /label="B"
     NLS             15..35
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     (<ahref=""urn:local:warddecaestecker:2t-c8811q6"">NLSSV40<
                     /a>)"
                     /label="SV40"
     CDS             45..101
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     GG              complement(102..112)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:294-ciz70s8"">C</a>"
                     /label="C"
     primer          complement(132..152)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        632..736
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    737..1597
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1768..2356
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          2720..2741
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac aatgcctaag aagaagagga aggttggaag cggagctact aacttcagcc
       61 tgctgaagca ggctggcgac gtggaggaga accctggacc tggcttgaga ccgaattctc
      121 gccctatagt gagtcgtatt acctggccgt cgttttacaa cgtcgtgact gggaaaaccc
      181 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      241 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      301 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
      361 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
      421 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
      481 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
      541 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      601 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      661 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      721 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      781 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      841 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      901 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      961 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1021 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1081 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1141 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1201 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1261 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     1321 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1381 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1441 cggtgagcgt ggctctcgag gtatcattgc agcactgggg ccagatggta agccctcccg
     1501 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     1561 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1621 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1681 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1741 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1801 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1861 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1921 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1981 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2041 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2101 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2161 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2221 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2281 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2341 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2401 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2461 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2521 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     2581 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     2641 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     2701 tcgtatgttg tgtggaattg tgagcggata acaatttcac acgagctatt taggtgacac
     2761 tatagaagtg aagctt
//
