LOCUS       pGG-C-UM120_0017.1-D        3825 bp    DNA     circular UNA 11-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.46-cn3y9rp
VERSION     urn.local.jonasblomme.46-cn3y9rp
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2i-c4nnd55"">C</a>"
                     /label="C"
     CDS             join(14..193,749..817,1019..1150)
                     /note
                     /label="UM120_0017.1"
     CDS             133
                     /created_by="jonasblomme"
                     /label="C>G silent mutation"
     intron          194..748
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          818..1018
                     /created_by="jonasblomme"
                     /label="Intron"
     GG              complement(1151..1161)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /label="D"
     primer          complement(1181..1201)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1681..1785
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1786..2646
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2817..3405
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3769..3790
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatgatcc acaacgtccg cactgtcaag ccggcatctt tcggcctcgc
       61 tgccccgaag gcccgtttcg cggctgctgc tcgcccatca gtgaaggcta acttcaaggt
      121 gaaactcgag acgcccgatg gaacaaagga ggtcgagatg aacggtgacg agtacatcgt
      181 tgaccaagct gaggtgcgcc atctcgtacg ccaatgccag ggcagcatgg agcgaagaag
      241 acttcatgaa ctgcgactca tatggcatcg tgggtgccgt ctagtagcat gcaaagctcg
      301 atgacagccg cgatctcacc ctgcattctc gacggtccga tgactggtca agctattcag
      361 caagccgagt atgccacgta tattaggcca catagtatct gccaccgcgg ctgtggcatt
      421 gtgatgtggt ggccttgcca gggaagatct gccgaggcaa tgcgagggtt caccctgcgc
      481 accctcggcg cattcgtggc ctcacactgc attggtcgcc tcacaacatg atgtgccgcg
      541 ccctcgtagc ttcggccacg ttctagcgca tgacatttgt atcatagtcc gggattccgt
      601 ttcactactc actgcaggct tgacggtgga tctgccggag gcctacgatg aattcccgtc
      661 ttgtgctttc acctgcgtcg atgtgcgtcg atgtacgctc ttcataaaaa atctattacg
      721 tactggccca ccaattttat gcttgcagga ggctggcatt gagctgccgt actcttgccg
      781 tgccggcacc tgctcttctt gcgccggtct ggttgtggta cgaccccacc ctcgaaggct
      841 ggaatcttca gcctgtgcgg tcatctgttc attagatcgc ttggcaggat gctcggactg
      901 tactctagtg cagctgtgca ccaagctgcc ttgtagtttc taataaatct agattcacca
      961 gcttgtgtca taatggtgtg ctggaggcca ccatgataaa cagtttgcca acttgcagga
     1021 gggtgaggtt gatcagtccg agcagaactt ccttgatgat gagcagatgg agaatggcta
     1081 tgtgctgacc tgcgttgctt acccaacctc ggaccttacc atcaagaccc accaggagga
     1141 ggagctgtac tcagtgagac cgaattctcg ccctatagtg agtcgtatta cctggccgtc
     1201 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     1261 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     1321 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
     1381 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     1441 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     1501 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     1561 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     1621 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     1681 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     1741 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     1801 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     1861 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     1921 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     1981 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     2041 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     2101 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     2161 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     2221 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     2281 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     2341 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     2401 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     2461 ggctggttta ttgctgataa atctggagcc ggtgagcgtg gctctcgagg tatcattgca
     2521 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     2581 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     2641 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     2701 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     2761 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2821 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2881 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2941 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3001 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3061 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3121 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3181 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3241 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3301 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3361 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3421 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3481 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3541 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     3601 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     3661 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     3721 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     3781 caatttcaca cgagctattt aggtgacact atagaagtga agctt
//
