LOCUS       pGG-C-UM119_0052.1-D        3893 bp    DNA     circular UNA 11-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.45-cn3y9rp
VERSION     urn.local.jonasblomme.45-cn3y9rp
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2i-c4nnd55"">C</a>"
                     /label="C"
     CDS             join(14..28,112..198,284..384,478..631,700..908,
                     1098..1218)
                     /note
                     /label="UM119_0052.1"
     intron          29..111
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          199..283
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          385..477
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          632..699
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          909..1097
                     /created_by="jonasblomme"
                     /label="Intron"
     CDS             960
                     /created_by="jonasblomme"
                     /label="G>C mutation"
     GG              complement(1219..1229)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /label="D"
     primer          complement(1249..1269)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1749..1853
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1854..2714
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2885..3473
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3837..3858
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatgaacg agagtgaggt gcgctttgac atgtcgtacc ggccgagtga
       61 agcgtctggt gcataacgcg atggaggcct tttgaaccac cgatcctgta ggtcaacaga
      121 cagattgaac agatggtgat gttcatcaag caggaagctg aggaaaaagc caacgaaatc
      181 cgcatgtctg ctgaggaggc gcgtcacccg cttgtccaga atgccttttg ttctgatgtt
      241 tcaacaaatt aactcagcgg ccgattaaaa tgcatgcatg caggaattta atctggaaaa
      301 gctcaacctg ctcgaaactg aaaagcagcg catccgcaaa gaatacgagc gcaaggaggc
      361 gcaggtggag gccaacaaaa agatgtgcgc agtcaaattt aagtgtgcgt gtgagccgga
      421 aaaatatggc attgcgtacc tcatatctca gcgttgaccc gaccgactgt gccgcagtga
      481 ggcctcgaag cagcataatg aacaacggct gaaagtgctt ggtgcaaagc agcgcttgat
      541 caaagacgtg ttacaggctg caaaggatga gttgaagaag aaagctggtg gtagtggtta
      601 cgctgatctc gtgagcgacc ttttggtgca ggtatgcttc gctgtttgcg tgcgctgtga
      661 cttgtaacat cgctctcaaa tgcacgtgac tatatttagg cgatgtacaa acttcaagag
      721 ccaagccaga tcgttcagtg ccgggaggca gatctgaagg tcatagagtc atgtgtgcca
      781 aaggcacagg agaagtataa aagtgtatat ggcagtgatg caccgcagct caccattgat
      841 cgcaagcact ttcttcccgc agctgcaaca tctcaggaac aagaggatga cccggatgcg
      901 cctacttggt gcggcagctg aatttgtgat cagctcgggt gataagttgt ttgtgatagc
      961 tctctttgca cagtttgtgt ttttctgctg catgtcgctc tctcatttac cattttacct
     1021 gagggacact ttctgactac ctgcggactt ggtctactaa caattacatt ctgctaattt
     1081 gagttgagtt tgtgcagcct gggaggtgtt gtggtgacct ctattagtgg ccgcattgtt
     1141 gtcgataaca cactggacgc caggctcaaa atcgtcgagg acaagcagct tccagcgatc
     1201 agagctcaat tgttcgagtc agtgagaccg aattctcgcc ctatagtgag tcgtattacc
     1261 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     1321 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     1381 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     1441 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     1501 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     1561 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     1621 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     1681 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1741 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1801 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1861 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1921 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1981 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     2041 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2101 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2161 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2221 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2281 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2341 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2401 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2461 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2521 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggc tctcgaggta
     2581 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2641 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2701 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2761 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2821 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2881 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2941 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3001 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3061 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3121 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3181 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3241 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3301 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3361 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3421 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3481 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3541 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3601 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3661 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     3721 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     3781 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     3841 gcggataaca atttcacacg agctatttag gtgacactat agaagtgaag ctt
//
