LOCUS       pGG-C-UM079_0010.1-D        4700 bp    DNA     circular UNA 11-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.43-cn3y9rp
VERSION     urn.local.jonasblomme.43-cn3y9rp
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2i-c4nnd55"">C</a>"
                     /label="C"
     CDS             join(14..226,313..759,1063..1197,1268..1655,1745..2025)
                     /note
                     /label="UM079_0010.1"
     intron          227..312
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          760..1062
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          1198..1267
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          1656..1744
                     /created_by="jonasblomme"
                     /label="Intron"
     GG              complement(2026..2036)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /label="D"
     primer          complement(2056..2076)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        2556..2660
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2661..3521
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3692..4280
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4644..4665
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatgccgt cgattgaaca agaggtcgca gaagcagcgg agcaatccca
       61 aatcagttct tcaggacaca ttgatgccga cactggaata gccgaaaagg aatccgggct
      121 acctgcagcg cttgatgctg caaacgcagt atctgcaccg caagcgacgc tgcgacgcga
      181 tcatgtatcc aacgctgtcg cgtttcttac aaattccaaa gttttggtaa gtggttctgt
      241 gctcttgctt gtccatttgt tgccaccact taacggatga cgttcttgac gcatcttcac
      301 agcatgccgc agagttcgag tgttggttcg aaacgggctt tcctgcaatc aaaggggctc
      361 acagatgagg agatcgatga ggcgtttcgg cgtactcagg gcatcgcacc tgtgccgcct
      421 gtgggtgctt ctgctcgcga tggagagcgg gatccccgag gacaagggcc ccagtcgacc
      481 acgaaatttc aggatgagac agcaatgcag caggtgaagt atacatggac ccagacattt
      541 atgggtacgg tggtgatggt gtgtgctgca tatggaatcg gcagtcttgc ttttccatat
      601 gctgtcaaag catggaagtg gctcaccagt tcggaacagc cagacagtag ctcagaagcc
      661 gaaacgaaga tgatcgagct gctgaccaag catgttcaac agcaagagga gacaaacaaa
      721 gacctcaaga gtgtgattga gactgtccgg aatattcagg tgcactggat catgctgtca
      781 ttattggcaa tttttttgtg tggcatgccg cacaaagcag gcgatctcgc agtatcaaca
      841 gttgtcagtg gtagtttcag cttgttgcgc tgctctgttt tgttttacat atggtccatg
      901 cgagggggat catgctcctt tttcacttca agatgtcctc attcaatgct cttggtgatg
      961 ctgtggttgc tcacacttgg gtcccactga atctgtacaa aagcttcagt tctctcctta
     1021 gccatggctt atttctgtat ggatctcaag cgtggcctgc aggcaaagcg agatgatgtc
     1081 agtggggcac acagcactgt tcctcaagta gaagctcagc tggactctgt gcgtcaggac
     1141 gttctggagc tggcaacagt tgtcaggtca aatgctgcta tgtccagtga aatcgcggtg
     1201 tggatctaga taatgaggag ttttgctact gtagttccca actcacgtca ggattgatca
     1261 catgcaggac ttgaaacgaa ctctgcagca gcaaccccca gcatcctcag tgaaggaatc
     1321 atctcgtgca cggtctgtac ctgtggactc tgattctgtg tcagtcggtc gtttcccaga
     1381 tcattttcct gccggagcag cagaccgtgc agcagctcct gctccgctgt ttgcaactgc
     1441 aacccctgca tctgcgtctc tgttcccagc tggagcagct ttgacaacaa gcaggcatca
     1501 ggatagcaat ggagcgagct ctgggttctt gacgcctgat tgcacagcag ggtcaagtgc
     1561 accgttgcag tatggggcaa catctgagag ctctgaacaa gccgaaccgt cgcacccaaa
     1621 cgaattttac agtatcttgg agatggtgaa caatggttaa tacgactcct actcttgctt
     1681 gtgatcaggt gttccccttt tgcctatgca tgtggttgag aggttttatt tgttctacat
     1741 gcaggtgtga caccagttgg tgtgcgtacg gacattgtcg acaaaccagt tgcaccacac
     1801 cttgaaccga caccttccag tatgccgcgg aaagccaagc catgggatac ggatggcagc
     1861 tctaaggcat ttgagctacc tggagctttg catgctatgt ctgttcagca ggacatggtg
     1921 gcacccagga ttagtgaagt atcatccaca caagatcttg atgccaacat gcagcatgat
     1981 gtggacggga gcgctgctcc gagggcggag cagagcaagg cacattcagt gagaccgaat
     2041 tctcgcccta tagtgagtcg tattacctgg ccgtcgtttt acaacgtcgt gactgggaaa
     2101 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     2161 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     2221 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     2281 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     2341 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     2401 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     2461 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     2521 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     2581 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     2641 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     2701 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     2761 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     2821 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2881 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2941 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     3001 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     3061 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     3121 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     3181 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     3241 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     3301 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     3361 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
     3421 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     3481 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     3541 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     3601 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3661 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3721 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3781 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3841 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3901 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3961 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4021 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4081 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4141 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4201 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4261 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4321 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4381 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     4441 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     4501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     4561 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     4621 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     4681 acactataga agtgaagctt
//
