LOCUS       pGG-C-UM025_0020.1-D        5954 bp    DNA     circular UNA 11-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.3z-cn3y9rn
VERSION     urn.local.jonasblomme.3z-cn3y9rn
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2i-c4nnd55"">C</a>"
                     /label="C"
     CDS             join(14..152,357..403,585..962,1718..1920,2402..2674,
                     3084..3279)
                     /note
                     /label="UM025_0020.1"
     intron          153..356
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          404..584
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          963..1717
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          1921..2401
                     /created_by="jonasblomme"
                     /label="Intron"
     CDS             2093
                     /created_by="jonasblomme"
                     /label="C>G mutation"
     intron          2675..3083
                     /created_by="jonasblomme"
                     /label="Intron"
     CDS             3135
                     /created_by="jonasblomme"
                     /label="C>G silent mutation"
     GG              complement(3280..3290)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /label="D"
     primer          complement(3310..3330)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        3810..3914
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    3915..4775
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      4946..5534
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          5898..5919
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatggatt atgacaatga cgacatgtat ggatcagagg acgaaaatga
       61 cgactactat cccgatcaag acgatgaaga ggaggacgag gacgaatcgg aggggcagaa
      121 ggactattct gaagacgagc cagaagatga tggtgcgcag ttgagcatat gcaacgttcc
      181 caatgtgcaa aggctaatac gataattggt gtttgtttat tctgtctaat tgcactgcat
      241 ggatatgctc tattatgcac ggtttgttgc cggcgctcct cggccggaat cacacaggct
      301 gcagctacag ctcatcgcca tatgcacacg tctgatgaca gggatggtgt gtgcagatga
      361 atgggagagc gcggaagact ggaaagcgcc tacggagttg cctgtgcggt cctgcgcaga
      421 ttgtcacggt cgacgacacc gctgttcgcc aatcatgcgg aaagcggact tacagcctca
      481 atcaatcgcg tcagagcatg cctatgtggc tactgttttg ctctcatact agttgcctaa
      541 gttcctaggc ccacgacgct gtcgagctcg gcgtgcgtgt gcaggacgca acctggaaaa
      601 gtattgcatc tcaagtcgac aagctagcca tccacaaccg gacggagctg acagtcctcc
      661 tcctaggcac tcctggcact ggctgctcat ccactgccaa ctcaatgctt gcggaaacga
      721 gggccgacgt gcggccattc attggcccgg gtgcagcaca gcgaccagag gttcacacac
      781 ggaggttgga atcatatggt tttctccttc gaataattga ctgtccgctg ttgtgcactt
      841 cgggctctct caaccttgaa gctctccagg acattgctgc tctaccagca gcctcagcag
      901 ttgatgtggt cctctacgtc gcccgcctag atgtcttcag agtgcagccc ctagagcgtc
      961 aggtgcgcag attcttgctg ctagtgccag ttccactgct gtagtcctgc atgtgcgtag
     1021 ctatgcgctg cggaccatac ctgtgccatc actgctgtag caactgcatt cctgatcgcc
     1081 gatacgagca cgttcaaact tcttgtctac agtaatgtcc aactttgtgg acagcactgt
     1141 ggctctatgg ctatcacgta gtcacaggtt gggatgttgg ccgacttgac cacagctgta
     1201 gtgatcacac agtcatccgc tggatggcat ctatctggtg gctgcgtttc cttttgcact
     1261 gtgccctgca aggcacccgc ttgttggact ttgcaccaaa agtcctgtga tcggatttgc
     1321 ctcagatttt gatatggcac tctggcactc tgcttggcat cgacacatgt atgcagcgac
     1381 tggtgcccaa tgcctcatca cagggggcag tgattgaact gtgctgtacc tgcatgttca
     1441 ctgggctctt ctaaccgcaa tgcgttcaaa gcacatcaac gattgcactg ttttaatcgg
     1501 cgatgtgacc caccctcgca cgcacagtgc atgttgacgg cccatggaca ggaccaacag
     1561 ccttgcactg ttgctccacc tgagccctct tgcgcccttg cggctctata acgtggttgc
     1621 agcacagcat gctcagcacg ggccagcgaa gcgggcacgg cgcgactcgc gtgtggcgct
     1681 gcgtgctgtt gtccccatca ttgcgtgctg cgtgcagctg atgtcggcga tctctgggca
     1741 cttcggcaag gacgtgtgga agcgcaccgt gctgtgcctg acgcgtggcg gcatcacgcc
     1801 tccgccgccg cacacgctgc cagacctcgc agcgcggcgc gcagagcagc tcggcggctg
     1861 cctgcgcaaa gccggcgccc cggcaggcct gccgtacacg ctcgttgaaa acagctctcg
     1921 gtgcgcccct acgccacaga acctgtgccc gggcacccac gcggctgccg accgcattct
     1981 ccggcgcata tcgtggaccg acaatttgca cgtggcatgc ggaaacgtca tgattgtggc
     2041 ttgaggttgc tgcgctgtcg atgtgacggt gcggagaggg gtgttggggt ctggtgccat
     2101 tgctaccccg catcgcgcag gcttgcacgc ctgcctgttt gctgtctgta accagagttc
     2161 cagtggtcat ctgatgagcg aatgggctga tcgtgctact ccagggcgtg gtgtacatgg
     2221 ccatgcatgg ggccaccagc ggcgatccga tccaagtttc cgcctcaatg agcgccttgg
     2281 cattttgtca gatgagtgga ggccatgccg cgccgcccgt acaccagcct tgccgcagcc
     2341 gctatgtgct tcatgtgcgt cgtcagcagc ggggcggtga tgctgcgtgc tgtggctaca
     2401 ggtgtccaac gtcgtcggac gggcagaagc agctgcgtga cggcacaaag tgggtgccgg
     2461 cgctgtggga ggcggtggtg gcgcgcgccg tcgcggccgg gccgcccaag gcgctgcgct
     2521 ttgcgccaca aaaccccaac cgccggcggc tgtggctcgt gccgctgctg gtcgccgcgc
     2581 agtacacact caaggtgttc gtgtttgacc gcgtcgtgga ggaggacagc tggcgaggcg
     2641 acaagaacgg cgagtatgac gcggagacac agcggtgcgc atcctggcct cgtgcgatga
     2701 ttctggtatc tagacagtat accacgaatc tcacaaagat cgtgatgcac ctcccttgat
     2761 cctggcctac acctccaatg cctccctcgc ggcaagagac acatgatttg actggcttca
     2821 tcttgcacga tatacggcat gagatcagtc tgtcagtctg ccatgcatga gatgcatgca
     2881 aggcagcttg tgtggctcgc cgtgttcttg tgctcttctt cgacttgttc ttgcccttct
     2941 caccgtggga tcttgttgca ttcgctgcgg cggccgcatc tggacgcctc tgtgcatcca
     3001 tcctgtgtgc tgcgctggca tcaacgtcta tgcattgcat aacctcaaca ggtgtcacca
     3061 cccggccgcg atcccgtgtg cagggaacac aagatgctcg cggacacgcg gcggcggcag
     3121 gcggaggagg agacgaagct gcggcggcag gtggaggcgc ggtcgcgtgc gcgcgcagag
     3181 gccgcccgcg gcgccatgag ctctgccgac cgcgagtccg gcttcgtgcc gtacgttgcc
     3241 gatgacgacg acggggacga ctacgaggac gatgaggact cagtgagacc gaattctcgc
     3301 cctatagtga gtcgtattac ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     3361 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     3421 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
     3481 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     3541 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     3601 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     3661 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     3721 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     3781 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3841 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3901 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     3961 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4021 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4081 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4141 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4201 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4261 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4321 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4381 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4441 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     4501 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4561 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4621 gtgagcgtgg ctctcgaggt atcattgcag cactggggcc agatggtaag ccctcccgta
     4681 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4741 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     4801 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     4861 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     4921 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     4981 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5041 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     5101 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5161 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5221 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5281 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5341 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5401 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5461 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5521 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     5581 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     5641 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     5701 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     5761 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     5821 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     5881 gtatgttgtg tggaattgtg agcggataac aatttcacac gagctattta ggtgacacta
     5941 tagaagtgaa gctt
//
