LOCUS       pGG-C-UM021_0090.1-D        4645 bp    DNA     circular UNA 11-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.3x-cn3y9rn
VERSION     urn.local.jonasblomme.3x-cn3y9rn
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2i-c4nnd55"">C</a>"
                     /label="C"
     CDS             join(14..118,620..1760,1861..1970)
                     /note
                     /label="UM021_0090.1"
     intron          119..619
                     /created_by="jonasblomme"
                     /label="Intron"
     CDS             595
                     /created_by="jonasblomme"
                     /label="G>C mutation"
     CDS             910
                     /created_by="jonasblomme"
                     /label="C>G silent mutation"
     intron          1761..1860
                     /created_by="jonasblomme"
                     /label="Intron"
     GG              complement(1971..1981)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /label="D"
     primer          complement(2001..2021)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        2501..2605
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2606..3466
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3637..4225
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4589..4610
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatgcgtg aggttatctc cattcacatc ggccaggccg gcatccaagt
       61 cggaaatgcc tgctgggagc tttattgtct tgagcacgga attcagcccg atgggcaggt
      121 acgtcttgtt cgagtttgcc tcgttcgttc gttcaggact ttcaggcagt tgcagctttg
      181 gaccccacaa tcgccattta caatcctcat atgtagtgga ttgttattgc tctgctttga
      241 ctatgatcac attttcaagt tcatcgaagc tattttgtct ctgacgctcg agttgcccga
      301 cactttgtga acgggtcgat acttcggatg ttaaaatgtg gctcaaatac catcaaatat
      361 gacgttatgc atctattacg ccgtttaaaa tatgcatagt ggttgtcgcg gcagctattg
      421 cttgtttcag acctaaattt aaaatagcgg ttgcaccaga cgctatttga aggcgtcccg
      481 caagccatgt ttgacttgaa agccatccgt attgtgaatt cgatgattta tctcgatcag
      541 ccttgaatga agtgtccgaa atgccatcgc gagcccattg tggcaacacg tgcgctctca
      601 cgacgtactg ctcttgcaga tgccttctga caagaccatc ggcggaggtg acgacgcttt
      661 caacaccttc ttctccgaga ctggtgctgg caaacatgtc cctcgtgctg ttttcctcga
      721 cttggagcct accgtcatcg acgaagtccg caccggcacc taccgccagc tgttccaccc
      781 cgagcagctc atctcaggca aggaggatgc cgctaacaac ttcgctcgtg gccactacac
      841 catcggcaag gagattgttg acctttgcct tgaccgcatt cgcaagcttg cggacaactg
      901 cacaggtctg cagggcttcc tggtcttcaa cgctgtcggt ggtggtaccg gctccggcct
      961 gggctctctc ctcctggagc gtctgtctgt tgactacggc aagaagtcca agctcggctt
     1021 caccgtctac ccgtcccctc aagtctcgac tgccgttgta gagccataca actctgtctt
     1081 gtccacccac tctctgctgg agcacactga tgttgctgtc atgcttgaca acgaggctgt
     1141 gtatgacatc tgccgccgct ctcttgacat cgagcgccct acatacacca acctgaacag
     1201 gctcatcgct caggtcattt catccctgac tgcttctctg cgatttgatg gtgccctcaa
     1261 cgttgatgtg accgagttcc agaccaacct tgtgccctac cctcgcatcc atttcatgct
     1321 ttcctcctac gcgcctgtca tctctgccga gaaggcatac catgagcagc tgtctgtggc
     1381 tgagatcacc aactctgcct tcgagcctgc ctccatgatg gctaagtgtg accctcgtca
     1441 tggcaagtac atggcctgct gcctgatgta ccgtggtgat gttgtcccca aggatgtcaa
     1501 tgccgctgtc gctaccatca agaccaagcg taccatccag tttgttgact ggtgccccac
     1561 cggcttcaag tgcggcatca actaccagcc acccacggtt gtcccaggag gtgaccttgc
     1621 caaggtacaa cgtgccgtgt gcatgatctc caactccacc gccatcgctg aggtgttctc
     1681 ccgcctggac cacaagttcg acctcatgta cgccaagcgt gccttcgtgc actggtatgt
     1741 cggcgagggc atggaggaag gtaagtcttt acgtccataa cttgttcgtc cttgcgctgc
     1801 catcgttccc attacaggct agatccattg tttccttgtt ctgaagatta aattgtgtag
     1861 gtgaattctc tgaggcccgt gaggatcttg ctgctctgga gaaggactac gaggaggttg
     1921 gtgctgagag tgctgagggt gagggtgaag aggaaggtga ggagggctac tcagtgagac
     1981 cgaattctcg ccctatagtg agtcgtatta cctggccgtc gttttacaac gtcgtgactg
     2041 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     2101 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     2161 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     2221 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     2281 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     2341 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     2401 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     2461 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2521 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2581 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2641 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2701 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2761 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2821 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     2881 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2941 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     3001 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     3061 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3121 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     3181 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3241 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3301 atctggagcc ggtgagcgtg gctctcgagg tatcattgca gcactggggc cagatggtaa
     3361 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3421 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3481 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3541 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3601 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3661 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3721 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3781 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3841 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3901 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3961 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4021 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4081 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4141 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4201 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4261 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4321 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4381 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     4441 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     4501 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     4561 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca cgagctattt
     4621 aggtgacact atagaagtga agctt
//
