LOCUS       pGG-C-UM017_0086.1-D        3842 bp    DNA     circular UNA 11-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.3w-cn3y9rn
VERSION     urn.local.jonasblomme.3w-cn3y9rn
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2i-c4nnd55"">C</a>"
                     /label="C"
     CDS             join(14..175,1063..1167)
                     /note
                     /label="UM017_0086.1"
     intron          176..1062
                     /created_by="jonasblomme"
                     /label="Intron"
     GG              complement(1168..1178)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /label="D"
     primer          complement(1198..1218)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1698..1802
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1803..2663
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2834..3422
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3786..3807
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatggctg ctgaccttgc gctcggcgag gaggtcttca gtgcaaactg
       61 cgctgtctgc cacagcggag gcaagaacgt gctcatcccc accaaaaccc tcgagaaggc
      121 cgctattgag aagtacctcg agggaggctt caacctttcc tctatcctgt accaggtgcg
      181 cgttcggcga atcgatgttg accgagttca agtgcataca gacaatttcc caagcgcccg
      241 gaacgagcat catgcacgtg acggcgccaa tgctaggcaa cggacgcttc gaagtccctg
      301 aatagacaga tctttgtaga agcgtggttg agcagtactt ctgggttggt tattgataac
      361 aaacgcagag gtaggggtgc ttctgtacga acaatctccg gtactagtac tgattcttgg
      421 gtacccgctg gatctgcagt accggcgcgc cggcagtacc cgcgatcgcg agctgcaccc
      481 acgtggcctt ggggactcga agcgagcgct gagtcacgcc ggtacctctg cgcgcagctg
      541 cgtacttgct atcgaaatcg agtttagact gaccggctgt gatatcgata gcatgggcat
      601 gaggcagcat ccaatactgc ctaggccaat catgcatgct catgatcaaa ccacatgcac
      661 cttgcacctt attgagttac aaaccactgt ccacgacata actcgtcact ggtcaccggt
      721 catcgcagcc tgtattagct gtgcgaccag cagcacccag tgtacctgct acttctttga
      781 cagttcgacg cgcactgaac acttggactt cgttgtgcaa catgccacga aggctgtcaa
      841 agtatgcgtg gctgcagcta gtggcactgg catgagttct gtcaggcatc atcatgatgg
      901 tatgtcagaa gccatacgtc tgccatcacc ttgatgagca aactggctca cagcagcgct
      961 tgacagtgca gactagtgat ggcttggctg aatagtgcca tgtccatagc ctgatctact
     1021 gaaggttaca ccaatgctga tattggagcg gtgggcatgc aggtgcagaa tggcaagggc
     1081 cagatgcccg cctgggaagg tgttcttgag gacgaggaga tcgatgctgt ctccaactac
     1141 gtctacgcga ccgcccaggc tggatggtca gtgagaccga attctcgccc tatagtgagt
     1201 cgtattacct ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     1261 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     1321 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
     1381 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     1441 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     1501 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     1561 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     1621 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     1681 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1741 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1801 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1861 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1921 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1981 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     2041 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     2101 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     2161 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     2221 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     2281 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     2341 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     2401 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     2461 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggct
     2521 ctcgaggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     2581 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     2641 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     2701 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2761 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2821 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2881 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2941 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3001 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3061 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3121 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3181 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3241 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3301 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3361 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3421 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3481 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3541 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3601 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     3661 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     3721 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     3781 gaattgtgag cggataacaa tttcacacga gctatttagg tgacactata gaagtgaagc
     3841 tt
//
