LOCUS       pGG-C-UM003_0201.1-D        3298 bp    DNA     circular UNA 11-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.3q-cn3y9rm
VERSION     urn.local.jonasblomme.3q-cn3y9rm
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2i-c4nnd55"">C</a>"
                     /label="C"
     CDS             join(14..342,433..472,585..623)
                     /note
                     /label="UM003_0201.1"
     intron          343..432
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          473..584
                     /created_by="jonasblomme"
                     /label="Intron"
     GG              complement(624..634)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /label="D"
     primer          complement(654..674)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1154..1258
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1259..2119
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2290..2878
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3242..3263
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatggctc gtaccaagca gaccgcacgt aagtccacag gtggcaaggc
       61 ccctcgtaag cagctcgcta ccaaggcagc tcgtaagtca gcccctgcta cgggcggtgt
      121 caagaagccc caccgctaca ggcccggtac cgtcgctctt cgtgagatcc gtaagtacca
      181 gaagtctact gagctcctca tccgcaagct gcctttccag cggctggtgc gtgagattgc
      241 tcaggactac aagacggacc tgcgtttcca gagctctgcc gtcctggcgc tgcaggaggc
      301 tgctgaggcc tacctggttg gcctgttcga ggacaccaac ttgtacgctc tcaatccggt
      361 tgatatattt tcaactttca atgcatttgt ttgtattgag tgctgtatca tcaagtcaac
      421 gtttgtgtgc aggtgtgcta tccacgccaa gcgcgtgact atcatgccca aggtaagctt
      481 ccggcgataa caaactatca aatttgtctt gtctcgagtt tcttgtgtcg gttgcctgtg
      541 aatgcgtctc cgcgccattc tgaccctttt tcgttgtttt gcaggacgtc cagctcgccc
      601 gccgcatccg tggtgagcgt gcctcagtga gaccgaattc tcgccctata gtgagtcgta
      661 ttacctggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      721 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      781 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
      841 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
      901 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
      961 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     1021 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     1081 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     1141 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1201 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1261 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1321 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1381 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     1441 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     1501 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     1561 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     1621 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1681 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1741 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1801 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1861 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1921 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg
     1981 aggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2041 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2101 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2161 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2221 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2281 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2341 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2401 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     2461 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2521 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2581 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2641 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2701 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2761 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2821 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2881 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2941 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3001 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3061 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     3121 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     3181 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     3241 tgtgagcgga taacaatttc acacgagcta tttaggtgac actatagaag tgaagctt
//
