LOCUS       pGG-D-YFP-SV40-E        3444 bp    DNA     circular SYN 04-APR-2023
DEFINITION  .
ACCESSION   KF718967
VERSION     KF718967.1
KEYWORDS    .
SOURCE      Cloning vector pGGD000 (unknown)
  ORGANISM  Cloning vector pGGD000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2j-c4nnd55"">D</a>"
                     /label="D"
     CDS             14..730
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /modified_by="jonasblomme"
                     /label="YFP"
     NLS             731..766
                     /modified_by="mamee"
                     /label="SV40"
     Stop            767..769
                     /created_by="mamee"
                     /label="STOP"
     GG              complement(770..780)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:29v-ciz7957"">E</a>"
                     /label="E"
     primer          complement(800..820)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1300..1404
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1405..2265
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2436..3024
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3388..3409
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcatca gctatggtga gcaagggcga ggagctgttc acgggcgtgg tgcccatcct
       61 ggtggagctg gacggcgacg tgaacggcca caagttcagc gtgtccggcg agggcgaggg
      121 cgacgccacc tacggcaagc tgaccctgaa gttcatctgc acgaccggca agctgcccgt
      181 gccctggccc accctggtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc
      241 cgaccacatg aagcagcacg acttcttcaa gagcgccatg ccggagggct acgtgcagga
      301 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgcggagg tgaagttcga
      361 gggcgacacg ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      421 catcctgggc cacaagctgg agtacaacta caacagccac aacgtgtaca tcatggccga
      481 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggctc
      541 ggtgcagctg gcggaccact accagcagaa cacccccatc ggcgacggcc cggtgctgct
      601 gcccgacaac cactacctgt cctaccagag cgccctgtcc aaggacccca acgagaagcg
      661 cgaccacatg gtgctgctgg agttcgtgac cgccgccggc atcaccctgg gcatggacga
      721 gctgtacaag atgtcaaggg ctgatcctaa gaagaagagg aaggtttgac tgctgagacc
      781 gaattctcgc cctatagtga gtcgtattac ctggccgtcg ttttacaacg tcgtgactgg
      841 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      901 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      961 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     1021 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     1081 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     1141 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     1201 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     1261 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1321 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1381 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1441 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1501 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1561 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1621 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     1681 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     1741 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1801 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     1861 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     1921 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     1981 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2041 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2101 tctggagccg gtgagcgtgg ctctcgaggt atcattgcag cactggggcc agatggtaag
     2161 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2221 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2281 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2341 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2401 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2461 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2521 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2581 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2641 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2701 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2761 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2821 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2881 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2941 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3001 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3061 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3121 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3181 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3241 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3301 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     3361 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac gagctattta
     3421 ggtgacacta tagaagtgaa gctt
//
