LOCUS pGG-D-YFP-N7-E 3657 bp DNA circular SYN 04-APR-2023 DEFINITION . ACCESSION KF718967 VERSION KF718967.1 KEYWORDS . SOURCE Cloning vector pGGD000 (unknown) ORGANISM Cloning vector pGGD000 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3749) AUTHORS Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U. and Forner,J. TITLE GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis JOURNAL PLoS ONE 8 (12), E83043 (2013) PUBMED 24376629 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 3749) AUTHORS Forner,J. TITLE Direct Submission JOURNAL Submitted (28-SEP-2013) Centre for Organismal Studies, Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230, Heidelberg D-69221, Germany COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##. FEATURES Location/Qualifiers GG 1..11 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="D" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; D" /label="D" CDS 14..730 /ApEinfo_revcolor="#9eafd2" /ApEinfo_fwdcolor="#9eafd2" /modified_by="jonasblomme" /label="YFP" NLS 731..979 /created_by="warddecaestecker" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on protein similarity" /Transferred_From="NLS N7" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (NLS N7)" /label="N7" Stop 980..982 /created_by="mamee" /label="STOP" GG complement(983..993) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="E" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; E" /label="E" primer complement(1013..1033) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)" /label="GG_SEQ_rev" promoter 1513..1617 /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" misc_feature 1618..2478 /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" rep_origin 2649..3237 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 3601..3622 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" ORIGIN 1 ggtctcatca gctatggtga gcaagggcga ggagctgttc acgggcgtgg tgcccatcct 61 ggtggagctg gacggcgacg tgaacggcca caagttcagc gtgtccggcg agggcgaggg 121 cgacgccacc tacggcaagc tgaccctgaa gttcatctgc acgaccggca agctgcccgt 181 gccctggccc accctggtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc 241 cgaccacatg aagcagcacg acttcttcaa gagcgccatg ccggagggct acgtgcagga 301 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgcggagg tgaagttcga 361 gggcgacacg ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 421 catcctgggc cacaagctgg agtacaacta caacagccac aacgtgtaca tcatggccga 481 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggctc 541 ggtgcagctg gcggaccact accagcagaa cacccccatc ggcgacggcc cggtgctgct 601 gcccgacaac cactacctgt cctaccagag cgccctgtcc aaggacccca acgagaagcg 661 cgaccacatg gtgctgctgg agttcgtgac cgccgccggc atcaccctgg gcatggacga 721 gctgtacaag gcggccgcat ctgaattcaa gcgtgaagag caagcaagga aagctaaggt 781 gaacaatgag aaaaagacgg aaatagtgaa accagagagt tgtagcaatg aaggagatgt 841 caaggatctg aaaagaaagg actctgagga tggaaacgag ggtgaggaag aagaagcttc 901 ttcgaaaccg aaaaagccaa aagttgctct ttctcatctt caggacattg acgacacaga 961 agctgatcaa gaagaagagt gactgctgag accgaattct cgccctatag tgagtcgtat 1021 tacctggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat 1081 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat 1141 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc 1201 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct 1261 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg 1321 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg 1381 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc 1441 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt 1501 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 1561 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 1621 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 1681 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 1741 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 1801 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 1861 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 1921 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 1981 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 2041 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 2101 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 2161 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 2221 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 2281 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggctctcga 2341 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 2401 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 2461 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 2521 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 2581 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 2641 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 2701 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 2761 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 2821 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 2881 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 2941 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 3001 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 3061 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 3121 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 3181 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 3241 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 3301 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 3361 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 3421 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 3481 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 3541 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt 3601 gtgagcggat aacaatttca cacgagctat ttaggtgaca ctatagaagt gaagctt //