LOCUS       pGG-B-USPA-C            2863 bp    DNA     circular UNA 04-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.6r-cgz8wqq
VERSION     urn.local.jonasblomme.6r-cgz8wqq
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2h-c4nnd55"">B</a>"
                     /label="B"
     CDS             join(12..84,170..188)
                     /SerialCloner_Color="&h008000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="False"
                     /SerialCloner_Arrow="True"
                     /modified_by="jonasblomme"
                     /label="USPA CTP with intron"
     intron          85..169
                     /SerialCloner_Color="&h8080FF"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="False"
                     /SerialCloner_Arrow="True"
                     /label="USPA intron1"
     GG              complement(189..199)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:294-ciz70s8"">C</a>"
                     /label="C"
     primer          complement(219..239)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        719..823
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    824..1684
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1855..2443
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          2807..2828
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac aatgtctgcc cttcgctgcg gcatggctgc gcctagcacc gctcaattga
       61 tgaaccggaa cgctcctcgg agaagtaagc tgggatgcct gcgctgggcg aaagctggca
      121 cggaatgagc ttcgcatcgg tactttaacc atgttggtat atactgcagg cgttgtcccg
      181 cgtgccgcgg cttgagaccg aattctcgcc ctatagtgag tcgtattacc tggccgtcgt
      241 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
      301 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      361 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
      421 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      481 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      541 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      601 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
      661 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      721 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      781 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      841 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      901 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      961 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     1021 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     1081 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     1141 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     1201 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1261 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1321 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1381 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1441 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1501 ctggtttatt gctgataaat ctggagccgg tgagcgtggc tctcgaggta tcattgcagc
     1561 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1621 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1681 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1741 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1801 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1861 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1921 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1981 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     2041 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2101 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2161 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2221 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2281 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2341 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2401 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2461 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2521 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2581 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     2641 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     2701 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     2761 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     2821 atttcacacg agctatttag gtgacactat agaagtgaag ctt
//
