LOCUS       pGG-B-HSP70c-C          3095 bp    DNA     circular UNA 03-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.6z-cgz8wqq
VERSION     urn.local.jonasblomme.6z-cgz8wqq
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2h-c4nnd55"">B</a>"
                     /label="B"
     CDS             join(12..53,199..248,394..420)
                     /SerialCloner_Color="&h8000FF"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="False"
                     /SerialCloner_Arrow="True"
                     /modified_by="jonasblomme"
                     /label="MTP HSP70C 2i"
     intron          54..198
                     /SerialCloner_Color="&h8080FF"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="False"
                     /SerialCloner_Arrow="True"
                     /label="HSP70C intron 1 wo bbsi"
     intron          249..393
                     /SerialCloner_Color="&h8080FF"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="False"
                     /SerialCloner_Arrow="True"
                     /label="HSP70C intron 2"
     GG              complement(421..431)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:294-ciz70s8"">C</a>"
                     /label="C"
     primer          complement(451..471)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        951..1055
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1056..1916
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2087..2675
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3039..3060
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac aatgcagcgc tcgggaggtc gcgtggtggc ggggctactt caggtgcgac
       61 tcaataactg atctgggcga gcgagtcagg gggcctcgaa acctctgggc acgtccatcg
      121 tgtgggcgtc ggcgcctcac gtctttgcaa ctcgcttgcg tcagctcaca ccgcgtatcg
      181 atttgattgg ggctgcagat tgcccgtgga tccactgcca gcgatgtggg cgcgcttcgt
      241 ggaatcaggt atgagcgtag cgtgttgcag ctcatcggtg acgaccgagt tagcacgcgt
      301 acattccggc agcgcgttac gctgctgtcg tcgttcggtg taaacgaagc cttctgctca
      361 tttggatgtt tttgtccgac aacccgtatg cagtgcctcg gcgggcgccc tttcggacgc
      421 ggcttgagac cgaattctcg ccctatagtg agtcgtatta cctggccgtc gttttacaac
      481 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      541 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      601 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
      661 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
      721 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
      781 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
      841 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
      901 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      961 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1021 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1081 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1141 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     1201 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     1261 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     1321 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     1381 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     1441 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1501 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1561 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1621 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1681 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1741 ttgctgataa atctggagcc ggtgagcgtg gctctcgagg tatcattgca gcactggggc
     1801 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     1861 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     1921 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1981 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2041 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2101 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2161 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2221 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2281 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2341 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2401 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2461 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2521 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2581 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2641 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2701 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2761 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2821 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     2881 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     2941 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     3001 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     3061 cgagctattt aggtgacact atagaagtga agctt
//
