LOCUS       pGG-B-cCA-C             2749 bp    DNA     circular UNA 03-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.6o-cgz8wqq
VERSION     urn.local.jonasblomme.6o-cgz8wqq
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2h-c4nnd55"">B</a>"
                     /label="B"
     CDS             12..74
                     /SerialCloner_Color="&h008040"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="note=""direct strand
                     translation""translation=""ARTGALLLVALALAGCAQAC"" \"
                     /modified_by="jonasblomme"
                     /modified_by="mamee"
                     /label="cCA"
     GG              complement(75..85)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:294-ciz70s8"">C</a>"
                     /label="C"
     primer          complement(105..125)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        605..709
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    710..1570
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1741..2329
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          2693..2714
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac aatggcgcgt actggcgctc tactcctggt cgcgctggcg cttgcgggct
       61 gcgcgcaggc ttgcggcttg agaccgaatt ctcgccctat agtgagtcgt attacctggc
      121 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
      181 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      241 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
      301 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
      361 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
      421 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
      481 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
      541 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
      601 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      661 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
      721 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
      781 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
      841 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
      901 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
      961 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     1021 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     1081 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     1141 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     1201 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     1261 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     1321 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     1381 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggctctc gaggtatcat
     1441 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     1501 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     1561 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     1621 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     1681 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1741 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1801 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1861 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1921 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1981 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2041 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2101 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2161 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2221 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2281 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2341 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     2401 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     2461 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     2521 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     2581 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     2641 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     2701 ataacaattt cacacgagct atttaggtga cactatagaa gtgaagctt
//
