LOCUS       pGG-B-BIP-C             2853 bp    DNA     circular UNA 03-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.6m-cgz8wqp
VERSION     urn.local.jonasblomme.6m-cgz8wqp
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2h-c4nnd55"">B</a>"
                     /label="B"
     CDS             join(12..45,111..178)
                     /modified_by="jonasblomme"
                     /label="BIP"
     intron          46..110
                     /created_by="jonasblomme"
                     /label="Intron"
     GG              complement(179..189)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:294-ciz70s8"">C</a>"
                     /label="C"
     primer          complement(209..229)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        709..813
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    814..1674
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1845..2433
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          2797..2818
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac aatggcgcag tggaaggctg ctgtgctgtt gctgggtagg tccgcgcgct
       61 gcgtcccgtc gcgctggatc tgtcatctaa ttataatgtt ttgctcgcag cgctggcctg
      121 cgcctcttac ggcttcggcg tttgggctga ggaggagaag ctcggcaccg ttattggtgg
      181 cttgagaccg aattctcgcc ctatagtgag tcgtattacc tggccgtcgt tttacaacgt
      241 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      301 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      361 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
      421 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
      481 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
      541 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
      601 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
      661 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
      721 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      781 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      841 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      901 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
      961 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     1021 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     1081 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1141 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1201 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1261 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1321 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     1381 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     1441 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     1501 gctgataaat ctggagccgg tgagcgtggc tctcgaggta tcattgcagc actggggcca
     1561 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     1621 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     1681 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     1741 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1801 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1861 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1921 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1981 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2041 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2101 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2161 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2221 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2281 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2341 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2401 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2461 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2521 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2581 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     2641 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     2701 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     2761 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacacg
     2821 agctatttag gtgacactat agaagtgaag ctt
//
