LOCUS       pGG-B-atpA-C            3088 bp    DNA     circular UNA 03-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.6k-cgz8wqp
VERSION     urn.local.jonasblomme.6k-cgz8wqp
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2h-c4nnd55"">B</a>"
                     /label="B"
     CDS             join(12..45,143..176,259..298,381..413)
                     /SerialCloner_Color="&h0D8E8C"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /modified_by="jonasblomme"
                     /label="atpA MTS with introns"
     intron          46..142
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          177..258
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          299..380
                     /created_by="jonasblomme"
                     /label="Intron"
     GG              complement(414..424)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:294-ciz70s8"">C</a>"
                     /label="C"
     primer          complement(444..464)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        944..1048
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1049..1909
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2080..2668
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3032..3053
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac aatgcgcagc caggcgcttt ctcttgcccg cgcgggtgag tccgggacaa
       61 ggtggcgtcg cgggtgtgcg cgcatggtcg gccggtgtcg tgactcggag cttgccccgc
      121 cgcaatgccc tcgcttttgc aggcctgctg cagctgagct cccagacggg tgccaggtaa
      181 gattgccggg tccatgcggg ctatatatgc tccgaaatcg atgcacttac acgtgcggac
      241 aatggtgttg ctctgcagcc tggagggtgg cttcgcgctg tccaagcgtg ccgagcaggt
      301 aagaagcttg atacgagcaa tggaaggggc tgcatgtgcg gtgccggaag cgctaatcgg
      361 atgtcgctgt tttccggtag gcgctcatcc gcgccagccg cgcgttcgcc agcggcttga
      421 gaccgaattc tcgccctata gtgagtcgta ttacctggcc gtcgttttac aacgtcgtga
      481 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
      541 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
      601 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
      661 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
      721 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
      781 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
      841 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
      901 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      961 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     1021 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     1081 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     1141 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     1201 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     1261 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     1321 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1381 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1441 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1501 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1561 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1621 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1681 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1741 taaatctgga gccggtgagc gtggctctcg aggtatcatt gcagcactgg ggccagatgg
     1801 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1861 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1921 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1981 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2041 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2101 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2161 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2221 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2281 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2341 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2401 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2461 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2521 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2581 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2641 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2701 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2761 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2821 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     2881 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     2941 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     3001 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacgagcta
     3061 tttaggtgac actatagaag tgaagctt
//
