LOCUS       pGG-D-tdTomato-E        4119 bp    DNA     circular SYN 03-APR-2023
DEFINITION  .
ACCESSION   KF718967
VERSION     KF718967.1
KEYWORDS    .
SOURCE      Cloning vector pGGD000 (unknown)
  ORGANISM  Cloning vector pGGD000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2j-c4nnd55"">D</a>"
                     /label="D"
     CDS             14..1444
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="tdTomato"
                     /Transferred_Similarity="94.19%"
                     /Primary_Match="94.19%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8s-dgycryy"">tdTomato</a>"
                     /modified_by="mamee"
                     /label="tdTomato*"
     GG              complement(1445..1455)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:29v-ciz7957"">E</a>"
                     /label="E"
     primer          complement(1475..1495)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1975..2079
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2080..2940
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3111..3699
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4063..4084
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcatca gctatgacca gcaagggcga ggaggtgatc aaggagttca tgcgcttcaa
       61 ggtgcgcatg gagggcagca tgaacggcca cgagttcgag atcgagggcg agggcgaggg
      121 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
      181 cttcgcctgg gacatcctga gcccccagtt catgtacggc agcaaggcct acgtgaagca
      241 ccccgccgac atccccgact acaagaagct gagcttcccc gagggcttca agtgggagcg
      301 cgtgatgaac ttcgaggacg gcggcctggt gaccgtgacc caggacagca gcctccagga
      361 cggcaccctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt
      421 gatgcagaag aagaccatgg gctgggaggc cagcaccgag cgcctgtacc cccgcgacgg
      481 cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt
      541 ggagttcaag accatctaca tggccaagaa gcccgtgcag ctgcccggct actactacgt
      601 ggacaccaag ctggacatca ccagccacaa cgaggactac accatcgtgg agcagtacga
      661 gcgctcggag ggccgccacc acctgttcct gggccacggc accggcagca ccggctcggg
      721 cagcagcggc accgccagca gcgaggacaa caacatggcc gtgatcaagg agttcatgcg
      781 cttcaaggtg cgcatggagg gctcgatgaa cggccacgag ttcgagatcg agggcgaggg
      841 cgagggccgc ccgtacgagg gcacgcagac ggcgaagctg aaggtgacga agggcggccc
      901 gctgccgttc gcgtgggaca tcctgtcgcc gcagttcatg tacggctcga aggcgtacgt
      961 gaagcacccg gcggacatcc cggactacaa gaagctgtcg ttccccgagg gcttcaagtg
     1021 ggagcgcgtg atgaacttcg aggacggcgg cctggtgacc gtgacgcagg actcgtcgct
     1081 ccaggacggc acgctgatct acaaggtgaa gatgcgcggc acgaacttcc cgccggacgg
     1141 ccccgtgatg cagaagaaga cgatgggctg ggaggcgtcc accgagcgcc tgtacccgcg
     1201 cgacggcgtg ctgaagggcg agatccacca ggcgctgaag ctgaaggacg gcggccacta
     1261 cctggtggag ttcaagacga tctacatggc gaagaagccc gtgcagctgc ccggctacta
     1321 ctacgtggac acgaagctgg acatcacgtc gcacaacgag gactacacga tcgtggagca
     1381 gtacgagcgc agcgagggcc gccaccacct gttcctgtac ggcatggacg agctgtacaa
     1441 gtaactgctg agaccgaatt ctcgccctat agtgagtcgt attacctggc cgtcgtttta
     1501 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     1561 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     1621 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     1681 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     1741 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     1801 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     1861 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     1921 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     1981 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2041 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2101 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2161 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2221 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     2281 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     2341 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2401 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     2461 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     2521 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     2581 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     2641 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     2701 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2761 tttattgctg ataaatctgg agccggtgag cgtggctctc gaggtatcat tgcagcactg
     2821 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     2881 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     2941 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3001 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3061 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3121 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3181 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3241 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3301 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3361 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3421 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3481 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3541 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3601 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3661 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3721 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3781 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3841 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     3901 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     3961 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     4021 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     4081 cacacgagct atttaggtga cactatagaa gtgaagctt
//
