LOCUS       pGG-D-mTagBFP2-E        3390 bp    DNA     circular SYN 03-APR-2023
DEFINITION  .
ACCESSION   KF718967
VERSION     KF718967.1
KEYWORDS    .
SOURCE      Cloning vector pGGD000 (unknown)
  ORGANISM  Cloning vector pGGD000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2j-c4nnd55"">D</a>"
                     /label="D"
     CDS             14..715
                     /SerialCloner_Color="&hFF0000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mTagBFP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; mTagBFP
                     (<a
                     href=""urn:local:jonasblomme:2d-cnsqc2r"">mTagBFP2</a>)"
                     /modified_by="jonasblomme"
                     /modified_by="mamee"
                     /label="mTagBFP*"
     GG              complement(716..726)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:29v-ciz7957"">E</a>"
                     /label="E"
     primer          complement(746..766)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1246..1350
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1351..2211
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2382..2970
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3334..3355
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcatca gctatgagcg agctgatcaa ggagaacatg cacatgaagc tgtacatgga
       61 gggcaccgtg gacaaccacc acttcaagtg caccagcgag ggcgagggca agccctacga
      121 gggcacccag accatgcgca tcaaggtggt ggagggcggc cccctgccct tcgccttcga
      181 catcctggcc acctcgttcc tgtacggcag caagaccttc atcaaccaca cccagggcat
      241 ccccgacttc ttcaagcaga gcttccccga gggcttcacc tgggagcgcg tgaccaccta
      301 cgaggacggc ggcgtgctga ccgccaccca ggacaccagc ctccaggacg gctgcctgat
      361 ctacaacgtg aagatccgcg gcgtgaactt caccagcaac ggccccgtga tgcagaagaa
      421 gaccctgggc tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaggg
      481 ccgcaacgac atggccctga agctggtggg cggcagccac ctgatcgcca acatcaagac
      541 cacctaccgc agcaagaagc ccgccaagaa cctgaagatg cccggcgtgt actacgtgga
      601 ctaccgcctg gagcgcatca aggaggccaa caacgagacg tacgtggagc agcacgaggt
      661 ggccgtggcc cgctactgcg acctgcccag caagctgggc cacaagctga actaactgct
      721 gagaccgaat tctcgcccta tagtgagtcg tattacctgg ccgtcgtttt acaacgtcgt
      781 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
      841 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
      901 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
      961 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     1021 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     1081 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     1141 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1201 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1261 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1321 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1381 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1441 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1501 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1561 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1621 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1681 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1741 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1801 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1861 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1921 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1981 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     2041 gataaatctg gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat
     2101 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2161 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2221 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2281 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2341 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2401 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2461 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2521 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2581 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2641 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2701 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2761 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2821 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2881 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2941 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3001 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3061 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3121 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3181 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     3241 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     3301 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc
     3361 tatttaggtg acactataga agtgaagctt
//
