LOCUS       pGG-C-mTagBFP2-D        3387 bp    DNA     circular SYN 03-APR-2023
DEFINITION  .
ACCESSION   KF718967
VERSION     KF718967.1
KEYWORDS    .
SOURCE      Cloning vector pGGD000 (unknown)
  ORGANISM  Cloning vector pGGD000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2i-c4nnd55"">C</a>"
                     /label="C"
     CDS             14..712
                     /SerialCloner_Color="&hFF0000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /modified_by="jonasblomme"
                     /label="mTagBFP"
     GG              complement(713..723)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /label="D"
     primer          complement(743..763)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1243..1347
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1348..2208
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2379..2967
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3331..3352
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatgagcg agctgatcaa ggagaacatg cacatgaagc tgtacatgga
       61 gggcaccgtg gacaaccacc acttcaagtg caccagcgag ggcgagggca agccctacga
      121 gggcacccag accatgcgca tcaaggtggt ggagggcggc cccctgccct tcgccttcga
      181 catcctggcc acctcgttcc tgtacggcag caagaccttc atcaaccaca cccagggcat
      241 ccccgacttc ttcaagcaga gcttccccga gggcttcacc tgggagcgcg tgaccaccta
      301 cgaggacggc ggcgtgctga ccgccaccca ggacaccagc ctccaggacg gctgcctgat
      361 ctacaacgtg aagatccgcg gcgtgaactt caccagcaac ggccccgtga tgcagaagaa
      421 gaccctgggc tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaggg
      481 ccgcaacgac atggccctga agctggtggg cggcagccac ctgatcgcca acatcaagac
      541 cacctaccgc agcaagaagc ccgccaagaa cctgaagatg cccggcgtgt actacgtgga
      601 ctaccgcctg gagcgcatca aggaggccaa caacgagacg tacgtggagc agcacgaggt
      661 ggccgtggcc cgctactgcg acctgcccag caagctgggc cacaagctga actcagtgag
      721 accgaattct cgccctatag tgagtcgtat tacctggccg tcgttttaca acgtcgtgac
      781 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
      841 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      901 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
      961 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     1021 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     1081 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     1141 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     1201 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     1261 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1321 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     1381 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     1441 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     1501 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     1561 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     1621 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     1681 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     1741 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     1801 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     1861 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     1921 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     1981 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     2041 aaatctggag ccggtgagcg tggctctcga ggtatcattg cagcactggg gccagatggt
     2101 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     2161 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     2221 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     2281 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2341 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2401 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2461 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2521 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2581 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2641 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2701 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2761 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2821 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2881 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2941 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3001 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3061 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3121 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     3181 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     3241 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     3301 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacgagctat
     3361 ttaggtgaca ctatagaagt gaagctt
//
