LOCUS       pGG-C-mCherryHDEL-D        3408 bp    DNA     circular SYN 03-APR-2023
DEFINITION  .
ACCESSION   KF718967
VERSION     KF718967.1
KEYWORDS    .
SOURCE      Cloning vector pGGD000 (unknown)
  ORGANISM  Cloning vector pGGD000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2i-c4nnd55"">C</a>"
                     /label="C"
     CDS             14..721
                     /SerialCloner_Color="&hFF0000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /modified_by="jonasblomme"
                     /label="mCherry"
     CDS             722..733
                     /created_by="jonasblomme"
                     /label="HDEL"
     GG              complement(734..744)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /label="D"
     primer          complement(764..784)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1264..1368
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1369..2229
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2400..2988
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3352..3373
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatggtgt ccaagggcga ggaggacaac atggccatca tcaaggagtt
       61 catgcgcttc aaggtgcaca tggagggcag cgtgaacggc cacgagttcg agatcgaggg
      121 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      181 cggccccctg cccttcgcct gggacatcct gagcccccag ttcatgtacg gcagcaaggc
      241 ctacgtgaag caccccgccg acatccccga ctacctgaag ctgagcttcc ccgagggctt
      301 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggacag
      361 cagcctccag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttccccag
      421 cgacggcccc gtgatgcaga agaagaccat gggctgggag gccagcagcg agcgcatgta
      481 ccccgaggac ggcgccctga agggcgagat caagcagcgc ctgaagctga aggacggcgg
      541 ccactacgac gccgaggtga agaccaccta caaggccaag aagcccgtgc agctgcccgg
      601 cgcctacaac gtgaacatca agctggacat caccagccac aacgaggact acaccatcgt
      661 ggagcagtac gagcgcgctg agggccgcca cagcaccggc ggcatggacg agctgtacaa
      721 gcacgacgag ctgtcagtga gaccgaattc tcgccctata gtgagtcgta ttacctggcc
      781 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
      841 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      901 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
      961 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     1021 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     1081 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     1141 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     1201 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     1261 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1321 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     1381 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     1441 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     1501 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     1561 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     1621 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     1681 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     1741 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1801 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1861 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1921 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1981 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2041 gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg aggtatcatt
     2101 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2161 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2221 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2281 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2341 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2401 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2461 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2521 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2581 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2641 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2701 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2761 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2821 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2881 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2941 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3001 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3061 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3121 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3181 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3241 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     3301 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     3361 taacaatttc acacgagcta tttaggtgac actatagaag tgaagctt
//
