LOCUS       pGG-C-mCherry-D         3396 bp    DNA     circular SYN 03-APR-2023
DEFINITION  .
ACCESSION   KF718967
VERSION     KF718967.1
KEYWORDS    .
SOURCE      Cloning vector pGGD000 (unknown)
  ORGANISM  Cloning vector pGGD000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2i-c4nnd55"">C</a>"
                     /label="C"
     CDS             14..721
                     /SerialCloner_Color="&hFF0000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /modified_by="jonasblomme"
                     /label="mCherry"
     GG              complement(722..732)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /label="D"
     primer          complement(752..772)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1252..1356
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1357..2217
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2388..2976
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3340..3361
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatggtgt ccaagggcga ggaggacaac atggccatca tcaaggagtt
       61 catgcgcttc aaggtgcaca tggagggcag cgtgaacggc cacgagttcg agatcgaggg
      121 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      181 cggccccctg cccttcgcct gggacatcct gagcccccag ttcatgtacg gcagcaaggc
      241 ctacgtgaag caccccgccg acatccccga ctacctgaag ctgagcttcc ccgagggctt
      301 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggacag
      361 cagcctccag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttccccag
      421 cgacggcccc gtgatgcaga agaagaccat gggctgggag gccagcagcg agcgcatgta
      481 ccccgaggac ggcgccctga agggcgagat caagcagcgc ctgaagctga aggacggcgg
      541 ccactacgac gccgaggtga agaccaccta caaggccaag aagcccgtgc agctgcccgg
      601 cgcctacaac gtgaacatca agctggacat caccagccac aacgaggact acaccatcgt
      661 ggagcagtac gagcgcgctg agggccgcca cagcaccggc ggcatggacg agctgtacaa
      721 gtcagtgaga ccgaattctc gccctatagt gagtcgtatt acctggccgt cgttttacaa
      781 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      841 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      901 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
      961 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     1021 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     1081 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     1141 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     1201 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     1261 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1321 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     1381 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     1441 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     1501 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     1561 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     1621 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     1681 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     1741 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     1801 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     1861 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     1921 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     1981 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     2041 attgctgata aatctggagc cggtgagcgt ggctctcgag gtatcattgc agcactgggg
     2101 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     2161 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     2221 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     2281 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     2341 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2401 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2461 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2521 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2581 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2641 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2701 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2761 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2821 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2881 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2941 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3001 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3061 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3121 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3181 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3241 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     3301 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     3361 acgagctatt taggtgacac tatagaagtg aagctt
//
