LOCUS pGG-C-GFP-D 3405 bp DNA circular SYN 03-APR-2023
DEFINITION .
ACCESSION KF718966
VERSION KF718966.1
KEYWORDS .
SOURCE Cloning vector pGGC000 (unknown)
ORGANISM Cloning vector pGGC000
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3749)
AUTHORS Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
and Forner,J.
TITLE GreenGate - A Novel, Versatile, and Efficient Cloning System for
Plant Transgenesis
JOURNAL PLoS ONE 8 (12), E83043 (2013)
PUBMED 24376629
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 3749)
AUTHORS Forner,J.
TITLE Direct Submission
JOURNAL Submitted (28-SEP-2013) Centre for Organismal Studies,
Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
Heidelberg D-69221, Germany
COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy
sequencing ##Assembly-Data-END##.
FEATURES Location/Qualifiers
GG 1..11
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="C"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; C"
/label="C"
CDS 14..730
/gene="ble/GFP fusion"
/note="Streptoalloteichus hindustanus ble gene fused with
Aequoria victoria GFP gene (synthetic Ulva codon adapted
construct)"
/codon_start=1
/transl_table=11
/product="bleomycin resistance protein/GFP fusion protein"
/protein_id="ABY60800.1"
/db_xref="GI:164523505"
/translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE
RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM
ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP
NEKRDHMVLLEFVTAAGITHGMDELYK"
/modified_by="jonasblomme"
/label="GFP"
GG complement(731..741)
/created_by="thjac"
/modified_by="thjac"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="D"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; D"
/label="D"
primer complement(761..781)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
/label="GG_SEQ_rev"
promoter 1261..1365
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
misc_feature 1366..2226
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
rep_origin 2397..2985
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 3349..3370
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
/label="GG_SEQ_fw"
ORIGIN
1 ggtctcaggc tccatggcca agggcgagga gctgttcacc ggtgtggtcc ccatcctggt
61 ggagctggac ggcgacgtga acggccacaa gttctccgtc tccggcgagg gtgagggtga
121 cgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
181 ctggcccacc ctggtcacca ccctgaccta cggtgtgcag tgcttctccc gctaccccga
241 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gagggctacg tgcaggagcg
301 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtca agttcgaggg
361 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
421 cctgggccac aagctggagt acaactacaa ctcccacaac gtgtacatca tggccgacaa
481 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggctccgt
541 gcagctggcc gaccactacc agcagaacac ccccatcggc gatggccccg tgctgctgcc
601 cgacaaccac tacctgtcca tccagtccgc cctgtccaag gaccccaacg agaagcgcga
661 ccacatggtc ctgctggagt tcgtcaccgc tgccggcatc acccacggca tggacgagct
721 gtacaagtaa tcagtgagac cgaattctcg ccctatagtg agtcgtatta cctggccgtc
781 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
841 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
901 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
961 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
1021 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
1081 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
1141 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
1201 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
1261 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
1321 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
1381 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
1441 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
1501 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
1561 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
1621 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
1681 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
1741 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
1801 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
1861 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
1921 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
1981 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
2041 ggctggttta ttgctgataa atctggagcc ggtgagcgtg gctctcgagg tatcattgca
2101 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
2161 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
2221 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
2281 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
2341 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
2401 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
2461 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
2521 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
2581 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
2641 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
2701 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
2761 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
2821 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
2881 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
2941 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
3001 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
3061 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
3121 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
3181 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
3241 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
3301 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
3361 caatttcaca cgagctattt aggtgacact atagaagtga agctt
//