LOCUS pGG-C-GFP-D 3405 bp DNA circular SYN 03-APR-2023 DEFINITION . ACCESSION KF718966 VERSION KF718966.1 KEYWORDS . SOURCE Cloning vector pGGC000 (unknown) ORGANISM Cloning vector pGGC000 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3749) AUTHORS Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U. and Forner,J. TITLE GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis JOURNAL PLoS ONE 8 (12), E83043 (2013) PUBMED 24376629 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 3749) AUTHORS Forner,J. TITLE Direct Submission JOURNAL Submitted (28-SEP-2013) Centre for Organismal Studies, Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230, Heidelberg D-69221, Germany COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##. FEATURES Location/Qualifiers GG 1..11 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="C" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; C" /label="C" CDS 14..730 /gene="ble/GFP fusion" /note="Streptoalloteichus hindustanus ble gene fused with Aequoria victoria GFP gene (synthetic Ulva codon adapted construct)" /codon_start=1 /transl_table=11 /product="bleomycin resistance protein/GFP fusion protein" /protein_id="ABY60800.1" /db_xref="GI:164523505" /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" /modified_by="jonasblomme" /label="GFP" GG complement(731..741) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="D" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; D" /label="D" primer complement(761..781) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)" /label="GG_SEQ_rev" promoter 1261..1365 /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" misc_feature 1366..2226 /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" rep_origin 2397..2985 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 3349..3370 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" ORIGIN 1 ggtctcaggc tccatggcca agggcgagga gctgttcacc ggtgtggtcc ccatcctggt 61 ggagctggac ggcgacgtga acggccacaa gttctccgtc tccggcgagg gtgagggtga 121 cgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 181 ctggcccacc ctggtcacca ccctgaccta cggtgtgcag tgcttctccc gctaccccga 241 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gagggctacg tgcaggagcg 301 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtca agttcgaggg 361 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 421 cctgggccac aagctggagt acaactacaa ctcccacaac gtgtacatca tggccgacaa 481 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggctccgt 541 gcagctggcc gaccactacc agcagaacac ccccatcggc gatggccccg tgctgctgcc 601 cgacaaccac tacctgtcca tccagtccgc cctgtccaag gaccccaacg agaagcgcga 661 ccacatggtc ctgctggagt tcgtcaccgc tgccggcatc acccacggca tggacgagct 721 gtacaagtaa tcagtgagac cgaattctcg ccctatagtg agtcgtatta cctggccgtc 781 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 841 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 901 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg 961 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag 1021 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc 1081 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt 1141 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag 1201 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg 1261 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 1321 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 1381 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 1441 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 1501 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 1561 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 1621 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 1681 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 1741 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 1801 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 1861 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 1921 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 1981 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 2041 ggctggttta ttgctgataa atctggagcc ggtgagcgtg gctctcgagg tatcattgca 2101 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 2161 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 2221 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 2281 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 2341 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 2401 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 2461 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 2521 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 2581 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 2641 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 2701 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 2761 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 2821 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 2881 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2941 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 3001 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 3061 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 3121 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 3181 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 3241 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 3301 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 3361 caatttcaca cgagctattt aggtgacact atagaagtga agctt //