LOCUS       pGG-E-UmtRbcS-F         3075 bp    DNA     circular SYN 03-APR-2023
DEFINITION  .
ACCESSION   KF718968
VERSION     KF718968.1  GI:568816384
KEYWORDS    .
SOURCE      Cloning vector pGGE000 (unknown)
  ORGANISM  Cloning vector pGGE000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START                                              
            ##Sequencing Technology :: Sanger dideoxy sequencing               
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2l-c4nnd55"">E</a>"
                     /label="E"
     terminator      12..400
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
                     /modified_by="jonasblomme"
                     /label="UmtRbcS"
     GG              complement(401..411)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:29z-ciz79d7"">F</a>"
                     /label="F"
     primer          complement(431..451)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        931..1035
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1036..1896
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2067..2655
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3019..3040
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcactg cgcagtcagg cagagtcgtt gttgggtgta tggttgcaca aaactagacc
       61 tcgagctggt cggttagaaa gagatgcttg tgcgactgga tgtaacaggc acaatccgag
      121 gaaaatgcaa cagaaacgtt atgatgtaac atctgagcac agtcaataaa attgcatgtt
      181 gtatgccgca cacaaaagtg ttgttaaaca tgcgatgtgg ttgtcaaatc attattcgca
      241 caaccatgga ccgaacctct ggcacgctgc atacgctgta ttcattcaca tcatgttttc
      301 tacttgtcac atgctctgca ccaagtaact ccgattagtt aggttcgcac atgtcatcgg
      361 agtggaccac ttcgaatcat ttggttcgaa gataaaccgg actatgagac cgaattctcg
      421 ccctatagtg agtcgtatta cctggccgtc gttttacaac gtcgtgactg ggaaaaccct
      481 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      541 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
      601 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
      661 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
      721 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
      781 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
      841 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
      901 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      961 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     1021 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     1081 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     1141 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     1201 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     1261 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     1321 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     1381 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     1441 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     1501 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     1561 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     1621 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     1681 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     1741 ggtgagcgtg gctctcgagg tatcattgca gcactggggc cagatggtaa gccctcccgt
     1801 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     1861 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1921 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1981 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2041 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2101 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2161 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2221 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2281 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2341 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2401 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2461 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2521 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2581 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2641 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2701 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     2761 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     2821 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     2881 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     2941 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     3001 cgtatgttgt gtggaattgt gagcggataa caatttcaca cgagctattt aggtgacact
     3061 atagaagtga agctt
//
