LOCUS pGG-C-bleRGFP-D 4271 bp DNA circular SYN 27-MAR-2023
DEFINITION .
ACCESSION KF718966
VERSION KF718966.1
KEYWORDS .
SOURCE Cloning vector pGGC000 (unknown)
ORGANISM Cloning vector pGGC000
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3749)
AUTHORS Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
and Forner,J.
TITLE GreenGate - A Novel, Versatile, and Efficient Cloning System for
Plant Transgenesis
JOURNAL PLoS ONE 8 (12), E83043 (2013)
PUBMED 24376629
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 3749)
AUTHORS Forner,J.
TITLE Direct Submission
JOURNAL Submitted (28-SEP-2013) Centre for Organismal Studies,
Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
Heidelberg D-69221, Germany
COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy
sequencing ##Assembly-Data-END##.
FEATURES Location/Qualifiers
GG 1..11
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="C"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; C"
/label="C"
CDS join(14..21,519..1596)
/gene="ble/GFP fusion"
/note="Streptoalloteichus hindustanus ble gene fused with
Aequoria victoria GFP gene (synthetic Ulva codon adapted
construct)"
/codon_start=1
/transl_table=11
/product="bleomycin resistance protein/GFP fusion protein"
/protein_id="ABY60800.1"
/db_xref="GI:164523505"
/translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE
RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM
ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP
NEKRDHMVLLEFVTAAGITHGMDELYK"
intron 22..518
/gene="ble/GFP fusion"
/note="derived from Ulva RbcS; enhancer"
/number=1
/label="RbcsI"
GG complement(1597..1607)
/created_by="thjac"
/modified_by="thjac"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="D"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; D"
/label="D"
primer complement(1627..1647)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
/label="GG_SEQ_rev"
promoter 2127..2231
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
misc_feature 2232..3092
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
rep_origin 3263..3851
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 4215..4236
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
/label="GG_SEQ_fw"
ORIGIN
1 ggtctcaggc tccatggcca agtgcgcatc gcgtgtcaat gctggcgctg ctgacgtccg
61 ctgcctaaac tgcatgaaag ctcctgcagt cgcaagtagt ccttttaact tgttgtatcg
121 gaacaaatgg ttgtagtctg tgccgtgtga cgatcttgag ccgcctggtc ggcaggtcca
181 atgtcggcct ggaccctgca caccctgccc accctgccgc tgctcacccg ttcacgaccc
241 cacaggtgct cgcagcagta attgtcgata gatagcaact gtgttctgac tattctttga
301 ccttccgcat ttgggactgc ggcggcacac ctactatccg caatgcacac cctggtgctg
361 gtactgcctg cctttccgcg cgagcccaca ccatgcaggc gcatagtaat ggactgtgat
421 tgatttaggc aactgtatga aacctcttca tcgtgccttg gttgcttgcg ataccctcac
481 gtgagttcat atgaccaacg ctggcacgac ccctgcagga tcaccagcgc cgttccggtg
541 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
601 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
661 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
721 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
781 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
841 ccggccggca actgcgtgca cttcgtggcc gaggagcagg tacccaaggg cgaggagctg
901 ttcaccggtg tggtccccat cctggtggag ctggacggcg acgtgaacgg ccacaagttc
961 tccgtctccg gcgagggtga gggtgacgcc acctacggca agctgaccct gaagttcatc
1021 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctgg tcaccaccct gacctacggt
1081 gtgcagtgct tctcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
1141 atgcccgagg gctacgtgca ggagcgcacc atcttcttca aggacgacgg caactacaag
1201 acccgcgccg aggtcaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
1261 atcgacttca aggaggacgg caacatcctg ggccacaagc tggagtacaa ctacaactcc
1321 cacaacgtgt acatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
1381 cgccacaaca tcgaggacgg ctccgtgcag ctggccgacc actaccagca gaacaccccc
1441 atcggcgatg gccccgtgct gctgcccgac aaccactacc tgtccatcca gtccgccctg
1501 tccaaggacc ccaacgagaa gcgcgaccac atggtcctgc tggagttcgt caccgctgcc
1561 ggcatcaccc acggcatgga cgagctgtac aagtaatcag tgagaccgaa ttctcgccct
1621 atagtgagtc gtattacctg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
1681 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
1741 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
1801 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
1861 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
1921 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
1981 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
2041 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
2101 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
2161 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
2221 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
2281 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
2341 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
2401 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
2461 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
2521 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
2581 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
2641 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
2701 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
2761 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
2821 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
2881 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
2941 agcgtggctc tcgaggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
3001 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
3061 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
3121 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
3181 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
3241 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
3301 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
3361 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
3421 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
3481 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
3541 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
3601 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
3661 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
3721 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
3781 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
3841 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
3901 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
3961 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
4021 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
4081 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
4141 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
4201 tgttgtgtgg aattgtgagc ggataacaat ttcacacgag ctatttaggt gacactatag
4261 aagtgaagct t
//