LOCUS pGG-C-bleRGFP-D 4271 bp DNA circular SYN 27-MAR-2023 DEFINITION . ACCESSION KF718966 VERSION KF718966.1 KEYWORDS . SOURCE Cloning vector pGGC000 (unknown) ORGANISM Cloning vector pGGC000 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3749) AUTHORS Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U. and Forner,J. TITLE GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis JOURNAL PLoS ONE 8 (12), E83043 (2013) PUBMED 24376629 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 3749) AUTHORS Forner,J. TITLE Direct Submission JOURNAL Submitted (28-SEP-2013) Centre for Organismal Studies, Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230, Heidelberg D-69221, Germany COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##. FEATURES Location/Qualifiers GG 1..11 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="C" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; C" /label="C" CDS join(14..21,519..1596) /gene="ble/GFP fusion" /note="Streptoalloteichus hindustanus ble gene fused with Aequoria victoria GFP gene (synthetic Ulva codon adapted construct)" /codon_start=1 /transl_table=11 /product="bleomycin resistance protein/GFP fusion protein" /protein_id="ABY60800.1" /db_xref="GI:164523505" /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" intron 22..518 /gene="ble/GFP fusion" /note="derived from Ulva RbcS; enhancer" /number=1 /label="RbcsI" GG complement(1597..1607) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="D" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; D" /label="D" primer complement(1627..1647) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)" /label="GG_SEQ_rev" promoter 2127..2231 /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" misc_feature 2232..3092 /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" rep_origin 3263..3851 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 4215..4236 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" ORIGIN 1 ggtctcaggc tccatggcca agtgcgcatc gcgtgtcaat gctggcgctg ctgacgtccg 61 ctgcctaaac tgcatgaaag ctcctgcagt cgcaagtagt ccttttaact tgttgtatcg 121 gaacaaatgg ttgtagtctg tgccgtgtga cgatcttgag ccgcctggtc ggcaggtcca 181 atgtcggcct ggaccctgca caccctgccc accctgccgc tgctcacccg ttcacgaccc 241 cacaggtgct cgcagcagta attgtcgata gatagcaact gtgttctgac tattctttga 301 ccttccgcat ttgggactgc ggcggcacac ctactatccg caatgcacac cctggtgctg 361 gtactgcctg cctttccgcg cgagcccaca ccatgcaggc gcatagtaat ggactgtgat 421 tgatttaggc aactgtatga aacctcttca tcgtgccttg gttgcttgcg ataccctcac 481 gtgagttcat atgaccaacg ctggcacgac ccctgcagga tcaccagcgc cgttccggtg 541 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc 601 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc 661 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc 721 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc 781 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac 841 ccggccggca actgcgtgca cttcgtggcc gaggagcagg tacccaaggg cgaggagctg 901 ttcaccggtg tggtccccat cctggtggag ctggacggcg acgtgaacgg ccacaagttc 961 tccgtctccg gcgagggtga gggtgacgcc acctacggca agctgaccct gaagttcatc 1021 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctgg tcaccaccct gacctacggt 1081 gtgcagtgct tctcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1141 atgcccgagg gctacgtgca ggagcgcacc atcttcttca aggacgacgg caactacaag 1201 acccgcgccg aggtcaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1261 atcgacttca aggaggacgg caacatcctg ggccacaagc tggagtacaa ctacaactcc 1321 cacaacgtgt acatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 1381 cgccacaaca tcgaggacgg ctccgtgcag ctggccgacc actaccagca gaacaccccc 1441 atcggcgatg gccccgtgct gctgcccgac aaccactacc tgtccatcca gtccgccctg 1501 tccaaggacc ccaacgagaa gcgcgaccac atggtcctgc tggagttcgt caccgctgcc 1561 ggcatcaccc acggcatgga cgagctgtac aagtaatcag tgagaccgaa ttctcgccct 1621 atagtgagtc gtattacctg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 1681 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 1741 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga 1801 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca 1861 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg 1921 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct 1981 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg 2041 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt 2101 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 2161 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 2221 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 2281 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 2341 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 2401 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 2461 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 2521 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 2581 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 2641 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 2701 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 2761 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 2821 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 2881 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 2941 agcgtggctc tcgaggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 3001 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 3061 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 3121 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 3181 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 3241 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 3301 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 3361 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 3421 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 3481 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 3541 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 3601 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 3661 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 3721 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 3781 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 3841 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 3901 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 3961 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 4021 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 4081 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 4141 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 4201 tgttgtgtgg aattgtgagc ggataacaat ttcacacgag ctatttaggt gacactatag 4261 aagtgaagct t //