LOCUS pGG-B-GS-RbcSI-C 3201 bp DNA circular SYN 27-MAR-2023 DEFINITION . ACCESSION KF718965 VERSION KF718965.1 KEYWORDS . SOURCE Cloning vector pGGB000 (unknown) ORGANISM Cloning vector pGGB000 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3749) AUTHORS Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U. and Forner,J. TITLE GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis JOURNAL PLoS ONE 8 (12), E83043 (2013) PUBMED 24376629 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 3749) AUTHORS Forner,J. TITLE Direct Submission JOURNAL Submitted (28-SEP-2013) Centre for Organismal Studies, Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230, Heidelberg D-69221, Germany COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##. FEATURES Location/Qualifiers GG 1..11 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="B" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; B" /label="B" CDS join(12..19,517..526) /gene="GS LINKER" /codon_start=1 /modified_by="mamee" /label="GS LINKER" intron 20..516 /gene="ble" /note="Ulva mutabilis RbcS intron 1; enhancer" /label="RbcsI" GG complement(527..537) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="C" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; C" /label="C" primer complement(557..577) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)" /label="GG_SEQ_rev" promoter 1057..1161 /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" misc_feature 1162..2022 /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" rep_origin 2193..2781 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 3145..3166 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" ORIGIN 1 ggtctcaaac aatgggcggg tgcgcatcgc gtgtcaatgc tggcgctgct gacgtccgct 61 gcctaaactg catgaaagct cctgcagtcg caagtagtcc ttttaacttg ttgtatcgga 121 acaaatggtt gtagtctgtg ccgtgtgacg atcttgagcc gcctggtcgg caggtccaat 181 gtcggcctgg accctgcaca ccctgcccac cctgccgctg ctcacccgtt cacgacccca 241 caggtgctcg cagcagtaat tgtcgataga tagcaactgt gttctgacta ttctttgacc 301 ttccgcattt gggactgcgg cggcacacct actatccgca atgcacaccc tggtgctggt 361 actgcctgcc tttccgcgcg agcccacacc atgcaggcgc atagtaatgg actgtgattg 421 atttaggcaa ctgtatgaaa cctcttcatc gtgccttggt tgcttgcgat accctcacgt 481 gagttcatat gaccaacgct ggcacgaccc ctgcaggggc ggcagcggct tgagaccgaa 541 ttctcgccct atagtgagtc gtattacctg gccgtcgttt tacaacgtcg tgactgggaa 601 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 661 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 721 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg 781 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca 841 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 901 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 961 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt 1021 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 1081 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 1141 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 1201 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 1261 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 1321 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 1381 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 1441 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 1501 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 1561 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 1621 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 1681 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 1741 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 1801 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 1861 ggagccggtg agcgtggctc tcgaggtatc attgcagcac tggggccaga tggtaagccc 1921 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 1981 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 2041 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 2101 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 2161 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 2221 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 2281 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 2341 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 2401 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 2461 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 2521 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 2581 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 2641 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 2701 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 2761 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 2821 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 2881 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 2941 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg 3001 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 3061 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 3121 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacgag ctatttaggt 3181 gacactatag aagtgaagct t //