LOCUS pGG-B-GS-RbcSI-C 3201 bp DNA circular SYN 27-MAR-2023
DEFINITION .
ACCESSION KF718965
VERSION KF718965.1
KEYWORDS .
SOURCE Cloning vector pGGB000 (unknown)
ORGANISM Cloning vector pGGB000
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3749)
AUTHORS Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
and Forner,J.
TITLE GreenGate - A Novel, Versatile, and Efficient Cloning System for
Plant Transgenesis
JOURNAL PLoS ONE 8 (12), E83043 (2013)
PUBMED 24376629
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 3749)
AUTHORS Forner,J.
TITLE Direct Submission
JOURNAL Submitted (28-SEP-2013) Centre for Organismal Studies,
Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
Heidelberg D-69221, Germany
COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy
sequencing ##Assembly-Data-END##.
FEATURES Location/Qualifiers
GG 1..11
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="B"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; B"
/label="B"
CDS join(12..19,517..526)
/gene="GS LINKER"
/codon_start=1
/modified_by="mamee"
/label="GS LINKER"
intron 20..516
/gene="ble"
/note="Ulva mutabilis RbcS intron 1; enhancer"
/label="RbcsI"
GG complement(527..537)
/created_by="thjac"
/modified_by="thjac"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="C"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; C"
/label="C"
primer complement(557..577)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
/label="GG_SEQ_rev"
promoter 1057..1161
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
misc_feature 1162..2022
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
rep_origin 2193..2781
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 3145..3166
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
/label="GG_SEQ_fw"
ORIGIN
1 ggtctcaaac aatgggcggg tgcgcatcgc gtgtcaatgc tggcgctgct gacgtccgct
61 gcctaaactg catgaaagct cctgcagtcg caagtagtcc ttttaacttg ttgtatcgga
121 acaaatggtt gtagtctgtg ccgtgtgacg atcttgagcc gcctggtcgg caggtccaat
181 gtcggcctgg accctgcaca ccctgcccac cctgccgctg ctcacccgtt cacgacccca
241 caggtgctcg cagcagtaat tgtcgataga tagcaactgt gttctgacta ttctttgacc
301 ttccgcattt gggactgcgg cggcacacct actatccgca atgcacaccc tggtgctggt
361 actgcctgcc tttccgcgcg agcccacacc atgcaggcgc atagtaatgg actgtgattg
421 atttaggcaa ctgtatgaaa cctcttcatc gtgccttggt tgcttgcgat accctcacgt
481 gagttcatat gaccaacgct ggcacgaccc ctgcaggggc ggcagcggct tgagaccgaa
541 ttctcgccct atagtgagtc gtattacctg gccgtcgttt tacaacgtcg tgactgggaa
601 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
661 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
721 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
781 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
841 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
901 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
961 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
1021 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
1081 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
1141 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
1201 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
1261 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
1321 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
1381 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
1441 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
1501 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
1561 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
1621 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
1681 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
1741 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
1801 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
1861 ggagccggtg agcgtggctc tcgaggtatc attgcagcac tggggccaga tggtaagccc
1921 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
1981 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
2041 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
2101 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
2161 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
2221 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
2281 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
2341 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
2401 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
2461 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
2521 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
2581 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
2641 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
2701 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
2761 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
2821 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
2881 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
2941 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
3001 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
3061 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
3121 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacgag ctatttaggt
3181 gacactatag aagtgaagct t
//