LOCUS       pGG-B-BleR-T2A-C        3618 bp    DNA     circular SYN 27-MAR-2023
DEFINITION  .
ACCESSION   KF718965
VERSION     KF718965.1
KEYWORDS    .
SOURCE      Cloning vector pGGB000 (unknown)
  ORGANISM  Cloning vector pGGB000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2h-c4nnd55"">B</a>"
                     /label="B"
     CDS             join(12..19,517..880)
                     /gene="ble"
                     /note="derived from Streptoalloteichus hindustanus,
                     adapted to Chlamydomonas reinhardtii and Ulva mutabilis
                     codon usage"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="ABY50194.1"
                     /db_xref="GI:163962062"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD"
                     /modified_by="mamee"
                     /label="bleR"
     intron          20..516
                     /label="RbcsI"
     CDS             881..943
                     /modified_by="jonasblomme"
                     /label="T2A"
     GG              complement(944..954)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:294-ciz70s8"">C</a>"
                     /label="C"
     primer          complement(974..994)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1474..1578
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1579..2439
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2610..3198
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3562..3583
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac aatggccaag tgcgcatcgc gtgtcaatgc tggcgctgct gacgtccgct
       61 gcctaaactg catgaaagct cctgcagtcg caagtagtcc ttttaacttg ttgtatcgga
      121 acaaatggtt gtagtctgtg ccgtgtgacg atcttgagcc gcctggtcgg caggtccaat
      181 gtcggcctgg accctgcaca ccctgcccac cctgccgctg ctcacccgtt cacgacccca
      241 caggtgctcg cagcagtaat tgtcgataga tagcaactgt gttctgacta ttctttgacc
      301 ttccgcattt gggactgcgg cggcacacct actatccgca atgcacaccc tggtgctggt
      361 actgcctgcc tttccgcgcg agcccacacc atgcaggcgc atagtaatgg actgtgattg
      421 atttaggcaa ctgtatgaaa cctcttcatc gtgccttggt tgcttgcgat accctcacgt
      481 gagttcatat gaccaacgct ggcacgaccc ctgcaggatc accagcgccg ttccggtgct
      541 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
      601 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
      661 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
      721 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
      781 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
      841 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac ggcagcggcg agggcagagg
      901 aagtcttcta acatgcggtg acgtggagga gaatccgggc cctggcttga gaccgaattc
      961 tcgccctata gtgagtcgta ttacctggcc gtcgttttac aacgtcgtga ctgggaaaac
     1021 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     1081 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     1141 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     1201 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     1261 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     1321 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     1381 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     1441 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     1501 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     1561 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     1621 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     1681 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     1741 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     1801 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     1861 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     1921 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     1981 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     2041 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     2101 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     2161 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     2221 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     2281 gccggtgagc gtggctctcg aggtatcatt gcagcactgg ggccagatgg taagccctcc
     2341 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     2401 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     2461 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     2521 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     2581 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2641 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2701 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     2761 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2821 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2881 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2941 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3001 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3061 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3121 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3181 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3241 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3301 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3361 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     3421 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     3481 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     3541 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacgagcta tttaggtgac
     3601 actatagaag tgaagctt
//
