LOCUS       pGG-B-BleR-E2A-C        3624 bp    DNA     circular SYN 27-MAR-2023
DEFINITION  .
ACCESSION   KF718965
VERSION     KF718965.1
KEYWORDS    .
SOURCE      Cloning vector pGGB000 (unknown)
  ORGANISM  Cloning vector pGGB000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2h-c4nnd55"">B</a>"
                     /label="B"
     CDS             join(12..19,517..880)
                     /gene="ble"
                     /note="derived from Streptoalloteichus hindustanus,
                     adapted to Chlamydomonas reinhardtii and Ulva mutabilis
                     codon usage"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="ABY50194.1"
                     /db_xref="GI:163962062"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD"
                     /modified_by="mamee"
                     /label="bleR"
     intron          20..516
                     /label="RbcsI"
     CDS             881..949
                     /label="E2A"
     GG              complement(950..960)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:294-ciz70s8"">C</a>"
                     /label="C"
     primer          complement(980..1000)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1480..1584
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1585..2445
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2616..3204
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3568..3589
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac aatggccaag tgcgcatcgc gtgtcaatgc tggcgctgct gacgtccgct
       61 gcctaaactg catgaaagct cctgcagtcg caagtagtcc ttttaacttg ttgtatcgga
      121 acaaatggtt gtagtctgtg ccgtgtgacg atcttgagcc gcctggtcgg caggtccaat
      181 gtcggcctgg accctgcaca ccctgcccac cctgccgctg ctcacccgtt cacgacccca
      241 caggtgctcg cagcagtaat tgtcgataga tagcaactgt gttctgacta ttctttgacc
      301 ttccgcattt gggactgcgg cggcacacct actatccgca atgcacaccc tggtgctggt
      361 actgcctgcc tttccgcgcg agcccacacc atgcaggcgc atagtaatgg actgtgattg
      421 atttaggcaa ctgtatgaaa cctcttcatc gtgccttggt tgcttgcgat accctcacgt
      481 gagttcatat gaccaacgct ggcacgaccc ctgcaggatc accagcgccg ttccggtgct
      541 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
      601 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
      661 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
      721 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
      781 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
      841 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac ggcagcggcc agtgcacgaa
      901 ctacgccctg ctgaagctgg cgggcgacgt ggagagcaac ccgggcccgg gcttgagacc
      961 gaattctcgc cctatagtga gtcgtattac ctggccgtcg ttttacaacg tcgtgactgg
     1021 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     1081 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     1141 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     1201 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     1261 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     1321 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     1381 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     1441 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1501 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1561 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1621 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1681 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1741 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1801 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     1861 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     1921 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1981 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2041 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2101 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2161 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2221 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2281 tctggagccg gtgagcgtgg ctctcgaggt atcattgcag cactggggcc agatggtaag
     2341 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2401 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2461 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2521 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2581 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2641 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2701 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2761 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2821 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2881 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2941 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3001 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3061 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3121 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3181 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3241 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3301 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3361 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3421 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3481 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     3541 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac gagctattta
     3601 ggtgacacta tagaagtgaa gctt
//
