LOCUS       pGG-A-UmpRbcS-B         3232 bp    DNA     circular SYN 27-MAR-2023
DEFINITION  .
ACCESSION   KF718964
VERSION     KF718964.1
KEYWORDS    .
SOURCE      Cloning vector pGGA000 (unknown)
  ORGANISM  Cloning vector pGGA000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2k-c4nnd55"">A</a>"
                     /label="A"
     promoter        12..557
                     /regulatory_class="promoter"
                     /note="Ulva RbcS promoter"
                     /modified_by="jonasblomme"
                     /label="UmpRbcS"
     GG              complement(558..568)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:28v-ciz6xqo"">B</a>"
                     /label="B"
     primer          complement(588..608)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1088..1192
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1193..2053
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2224..2812
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3176..3197
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc tcgttgttcg tacacaagga tgtcgcttgt ggctttttcg ctcggctttc
       61 caggagggca gtccagatac catgcggccg ccgtgtccat cactcgcgct gcagctgatg
      121 tgggcagata ctgcactaat ctgccgcacg acgcgtcggc ctgagtcggc ggacaccgga
      181 ggcttgctgg agtactggca caagtgtgcg tgtgattgcc agaaacagca acccactcac
      241 tagatatacc gctccaataa tgggttcatg tgcaagctca ctcaataacg aaacgcttca
      301 ctattgtgca cgtgccgggg ttgcttgggt atggcgtgcc atacgggttc tgcacatgtc
      361 acccacgtgg gtgtggcacg agatatcgag gtggccatgt aaaaggcatt tgctagaggg
      421 cagctcgtcc atgccgctcg agcacaccga gtgcgcacca taaatgcagc acatgcctgt
      481 gcagcctatc tcctgtctct gttacatccc accacctcct cacatatccc cttccaccct
      541 taaaccgcac agcagacaac atgagaccga attctcgccc tatagtgagt cgtattacct
      601 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      661 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      721 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
      781 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
      841 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
      901 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
      961 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     1021 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     1081 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1141 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1201 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1261 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1321 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     1381 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     1441 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     1501 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     1561 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     1621 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     1681 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     1741 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     1801 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     1861 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggct ctcgaggtat
     1921 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     1981 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2041 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2101 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2161 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2221 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2281 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2341 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     2401 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2461 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2521 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2581 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2641 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2701 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2761 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2821 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2881 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     2941 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     3001 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     3061 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     3121 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     3181 cggataacaa tttcacacga gctatttagg tgacactata gaagtgaagc tt
//
