LOCUS       pGG-A-UmpRbcS+Intron-B        3729 bp    DNA     circular SYN 27-MAR-2023
DEFINITION  .
ACCESSION   KF718964
VERSION     KF718964.1
KEYWORDS    .
SOURCE      Cloning vector pGGA000 (unknown)
  ORGANISM  Cloning vector pGGA000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2k-c4nnd55"">A</a>"
                     /label="A"
     promoter        12..1054
                     /regulatory_class="promoter"
                     /note="Ulva RbcS promoter"
                     /modified_by="jonasblomme"
                     /label="UmpRbcS"
     intron          438..934
                     /label="RbcsI"
     GG              complement(1055..1065)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:28v-ciz6xqo"">B</a>"
                     /label="B"
     primer          complement(1085..1105)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1585..1689
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1690..2550
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2721..3309
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3673..3694
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc tcgttgttcg tacacaagga tgtcgcttgt ggctttttcg ctcggctttc
       61 caggagggca gtccagatac catgcggccg ccgtgtccat cactcgcgct gcagctgatg
      121 tgggcagata ctgcactaat ctgccgcacg acgcgtcggc ctgagtcggc ggacaccgga
      181 ggcttgctgg agtactggca caagtgtgcg tgtgattgcc agaaacagca acccactcac
      241 tagatatacc gctccaataa tgggttcatg tgcaagctca ctcaataacg aaacgcttca
      301 ctattgtgca cgtgccgggg ttgcttgggt atggcgtgcc atacgggttc tgcacatgtc
      361 acccacgtgg gtgtggcacg agatatcgag gtggccatgt aaaaggcatt tgctagaggg
      421 cagctcgtcc atgccgcgtg cgcatcgcgt gtcaatgctg gcgctgctga cgtccgctgc
      481 ctaaactgca tgaaagctcc tgcagtcgca agtagtcctt ttaacttgtt gtatcggaac
      541 aaatggttgt agtctgtgcc gtgtgacgat cttgagccgc ctggtcggca ggtccaatgt
      601 cggcctggac cctgcacacc ctgcccaccc tgccgctgct cacccgttca cgaccccaca
      661 ggtgctcgca gcagtaattg tcgatagata gcaactgtgt tctgactatt ctttgacctt
      721 ccgcatttgg gactgcggcg gcacacctac tatccgcaat gcacaccctg gtgctggtac
      781 tgcctgcctt tccgcgcgag cccacaccat gcaggcgcat agtaatggac tgtgattgat
      841 ttaggcaact gtatgaaacc tcttcatcgt gccttggttg cttgcgatac cctcacgtga
      901 gttcatatga ccaacgctgg cacgacccct gcagtcgagc acaccgagtg cgcaccataa
      961 atgcagcaca tgcctgtgca gcctatctcc tgtctctgtt acatcccacc acctcctcac
     1021 atatcccctt ccacccttaa accgcacagc agacaacatg agaccgaatt ctcgccctat
     1081 agtgagtcgt attacctggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     1141 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     1201 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     1261 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     1321 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     1381 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     1441 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     1501 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     1561 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     1621 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     1681 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     1741 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     1801 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     1861 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     1921 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1981 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     2041 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     2101 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     2161 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     2221 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     2281 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     2341 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     2401 cgtggctctc gaggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     2461 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     2521 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     2581 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     2641 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2701 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2761 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2821 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     2881 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2941 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3001 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3061 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3121 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3181 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3241 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3301 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3361 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3421 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3481 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     3541 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     3601 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     3661 ttgtgtggaa ttgtgagcgg ataacaattt cacacgagct atttaggtga cactatagaa
     3721 gtgaagctt
//
