LOCUS       pGG-A-pUpACT1-B         4627 bp    DNA     circular SYN 27-MAR-2023
DEFINITION  .
ACCESSION   KF718964
VERSION     KF718964.1
KEYWORDS    .
SOURCE      Cloning vector pGGA000 (unknown)
  ORGANISM  Cloning vector pGGA000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2k-c4nnd55"">A</a>"
                     /label="A"
     promoter        12..1952
                     /label="pUpACT1"
     GG              complement(1953..1963)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:28v-ciz6xqo"">B</a>"
                     /label="B"
     primer          complement(1983..2003)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        2483..2587
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2588..3448
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3619..4207
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4571..4592
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc taagggactg taagaaggtg cattcaaact aattaagata tcaagataca
       61 agattcgagt tcaggtcaag tagtaccaag tcaagatcaa gcaagactgg cgttctcata
      121 ggtactatag ggcatggtat tagcacttaa gactgcatca attcgcgtgg tgacagttag
      181 ttcagtttat tccctattac ttgagctcat aagcttgtct tgtctaggcc actcacatgc
      241 ctagctgcct tggaaccatc ctggttcagg gccttgcaag atcatgacaa agactactaa
      301 tccagtgcag agaagtattt gtgcccgcac agcttgttag ggagggtgtc tatgcgagtt
      361 aacgggtaag tgttgttaat cgtgttctca ttgaggtatc taaagtcaat gcacaatccg
      421 tcagtttgcc ccccggtttg ggtgcaaata acattcgagg cccatgggga taatgaaggt
      481 gttactcatc ccatgtcaag cacgtgttgt aattgtttct caagttctaa ggattctctg
      541 ggagtgaggc aatactgtct ggccgccttt ggtcatgcac ctggtagcag ctcaatgtcc
      601 atttcgaatc tgttgcgaaa gtgttggttg gtgttgccca ggttgccgct tgcagcctaa
      661 tgcttggtga tgacctcctg aaggacacac atcgcgggcc tatgaggacc atcaaacaaa
      721 tcatatcagc acgtgcacgc agatagcatc tacgtgctga agccgctgat gacgtggcgt
      781 ttgtccatca gaacaactgc acacagaaca gttaccagac taaatattag ctccaatagg
      841 gatccgtgtg tctgaatctc cgtgcgttgt gatgtgcacc atactcaact ggagtatctt
      901 gcaagcattt catgcctcac atctgtgcac agctgccatc tgtatcatga ccacccaagc
      961 tcagccagtc atcttgaggt gcaaaacgaa acaacattgc ctgaccagag attgtatctt
     1021 cccgaagtcc gggagtgcaa ttttcgcagc atgggataca gccagttggc aggcctcaac
     1081 atccacgtca cgctgccttc gacatcgggg tcagtcggga agtacacacg tccccgaact
     1141 cgggattacc accaggttgc agagccctta gtcgagacaa attcgtgaag cggttcagtt
     1201 ggcgtcgggt ttggctcgag ttctcggcct ttgtacacaa cggtccagaa tatgaatccc
     1261 atgcccaaag tacgtcgcgc atggttcgtc gatcgagcag aagcatgcga aatgttgttg
     1321 acgtggtcct tgaagaatga gacaaaccct ggcaagctgc agctgagaaa ctgttaggta
     1381 ttattcctga caagagaacc cggtgcaaaa tttagattgt cgtaatgtgc ataattcctg
     1441 cgagaggcat gagggcatga agcccaacag catgtcaagc aacagatgga actgtcatgc
     1501 aagcgcaagt cgaaattgtt tgtttgtgaa tctcctactt tgcttagtca tgttcagacc
     1561 ttctggaaca ttcattggcg ttctgagcgc agtctggcag cgcgtttgac cgaccgctcg
     1621 atgaactcgg agacacctgg acctgcatat aacatgattc tatgccaacc ccattttgct
     1681 caaccaaaca tttttcggat ccgtacaata ttgcttctga aaagtcaacg gctgttcgac
     1741 attagcactc ggccgcaacg gtgaggtcaa ttgttcgccg gtaaagcgtt ccttgccgat
     1801 cgtgagatat gccgtgccgc agactcatca atggcggatt tcatatgcct tttgaaggat
     1861 gattgttgcg ggtgtttgtg tggcatgttc gtgttcattc atgggagttc agatgggtgc
     1921 atgcagaaaa agtagacgtc taaacctgga gaaacatgag accgaattct cgccctatag
     1981 tgagtcgtat tacctggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     2041 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     2101 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     2161 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     2221 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     2281 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     2341 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     2401 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     2461 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     2521 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     2581 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     2641 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     2701 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     2761 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     2821 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     2881 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     2941 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     3001 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     3061 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     3121 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     3181 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     3241 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     3301 tggctctcga ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     3361 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     3421 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     3481 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     3541 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     3601 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3661 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3721 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3781 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3841 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3901 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3961 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4021 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4081 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4141 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4201 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4261 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     4321 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     4381 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     4441 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     4501 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     4561 gtgtggaatt gtgagcggat aacaatttca cacgagctat ttaggtgaca ctatagaagt
     4621 gaagctt
//
