LOCUS       pGG-A-pUmACT1-B         4686 bp    DNA     circular SYN 27-MAR-2023
DEFINITION  .
ACCESSION   KF718964
VERSION     KF718964.1
KEYWORDS    .
SOURCE      Cloning vector pGGA000 (unknown)
  ORGANISM  Cloning vector pGGA000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2k-c4nnd55"">A</a>"
                     /label="A"
     promoter        12..2011
                     /label="pUmACT1"
     GG              complement(2012..2022)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:28v-ciz6xqo"">B</a>"
                     /label="B"
     primer          complement(2042..2062)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        2542..2646
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2647..3507
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3678..4266
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4630..4651
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc taggccaccc agtgcatctt tgatggagcc tcgtggcagt gctgttgatg
       61 cagcagacgg gctgatgaca aggatgctgt ttgcggtcta ttgtggtggt gccaccgcat
      121 gcttagcatg gagtgcactg ccacacagcg tgctctatac aacacgaaag ttctttgtta
      181 tgtcatgcaa tcttggaaaa cttgcttgtg cccaggatgg aaatcatacc aaaggaattg
      241 ggaaatgcgc caggccaggc gggcacgtgt gagcttggtg aagagcagac tgagaagcgc
      301 ccgcacatgt ggacttctgc gtggacttgt tgtgctgggt tggtatgctg tgtgtgttac
      361 agagtgtcgt tatgcgctag gcatgccagt agcacatccg atgcactacc tcacaactca
      421 gcaaccgggc ggactctgcc cgccatggct gcctttcctg ggctgctgtg ctgtgtttgt
      481 gtgtgcgctg ccggttgtgt tccttgtgag tgctgaggtg tagggtggcc atgctgcttg
      541 tgattatttg tttagttccg tgctttcttc ctccagcgct gctggggcct acctctatca
      601 cagtgtaaca aatgtggaca tctggaggaa ggcctgcacg gccgtatacg gacaagttgg
      661 cacagacagc atgcatgggt gcggaatggc actttcccga caagtgcaag gttatgtaag
      721 gcctggccag attgtcttga tgaactgcgc attgattggt gcgattctct tgcacaagac
      781 ttttgccagt gtacacaact atcgcagctg cctgaccttt ctgacacaaa catcggagac
      841 atcgctcttc caggaaatat attgcagcaa tcctgccacg aggcatacgc gtagcttgcg
      901 tgcggtggcc ttgaatcagt gcagcacaga tctcagctgc agcggtgagc atgcctcgcc
      961 aagttcaaat cgcacgagac gcgtggtgca tactgttaca tcgaggggct ccgatccagt
     1021 gcaccaagcg aacgtacaac tagagtctag actcacatgc tacccagcag atgcgagccg
     1081 gccaggcgat cacctggcac aaactgcgca cccgtgagcc gtgctaccct ggacatggtc
     1141 tttctgatca caaacccgtt tcctttcact gtcgccgcac tgaactcccg cgtccagcat
     1201 acttatgcat atatgttatg tataagcagg gccacacccg tcgtgacgcc taggccgcgc
     1261 ctgcgcggcc tcttgtcgcg gagatcgcag tgaagctcat agagctggca aggcatcacg
     1321 ctccctgtgc agaatggggc tgttcggcat atgggtgtca cataggttgc gtgtatcgtg
     1381 ccaatctcgc actgaccgcc atcacaccgt ttttgacgct tcatgattgc aagcagtatg
     1441 agctcatcgc ataggtgccc tgttgtaatc gacgtgggga caagctacac caaagctggt
     1501 aaggactcaa acgtaggctc gaccgtgcac cacatcagcg tgcaaccaga tcacggcact
     1561 ccaaccgtgc gatgcaggct ttggtggtaa cttggatcct tcggccgtgt tttcaagcag
     1621 tgtctccgct tcacgtagcg agaatacgcc actgagccat tcacccagca ccgccgcggc
     1681 caagcccaaa tcgtcatacc caatcaagaa cggggtggtc gtaaattgga gtgactttga
     1741 ggagctcgta tctgaatgct ttcatgagtg cgcgcctcga ggctaacacg gactgtcacc
     1801 atcccatgtt gagcgcaccg tcagcttgca attcaaggtc gcccatcata tctgttagac
     1861 ctgtgctatg attaaggata agctgcagtc cgaccaggaa agtgacttgg ctgggcgctg
     1921 ttccttcccc atgcaaccca gcagattatc tgcgagacga tgcgttatgt gcaggcgcct
     1981 ggtggtggat agcagagagc acccagtgct caacatgaga ccgaattctc gccctatagt
     2041 gagtcgtatt acctggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     2101 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     2161 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     2221 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     2281 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     2341 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     2401 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     2461 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     2521 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     2581 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     2641 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     2701 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     2761 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     2821 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     2881 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     2941 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     3001 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     3061 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     3121 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3181 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     3241 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     3301 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     3361 ggctctcgag gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     3421 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     3481 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     3541 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3601 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     3661 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3721 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3781 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3841 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3901 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3961 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4021 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4081 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4141 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4201 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4261 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4321 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     4381 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     4441 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     4501 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     4561 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     4621 tgtggaattg tgagcggata acaatttcac acgagctatt taggtgacac tatagaagtg
     4681 aagctt
//
