LOCUS       pGG-A-pUM140_0016+Intron-B        5117 bp    DNA     circular SYN 27-MAR-2023
DEFINITION  .
ACCESSION   KF718964
VERSION     KF718964.1
KEYWORDS    .
SOURCE      Cloning vector pGGA000 (unknown)
  ORGANISM  Cloning vector pGGA000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2k-c4nnd55"">A</a>"
                     /label="A"
     promoter        12..2442
                     /modified_by="jonasblomme"
                     /modified_by="mamee"
                     /label="pUM140_0016"
     intron          1802..2298
                     /label="RbcsI"
     GG              complement(2443..2453)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:28v-ciz6xqo"">B</a>"
                     /label="B"
     primer          complement(2473..2493)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        2973..3077
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    3078..3938
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      4109..4697
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          5061..5082
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc ttgccgaatt cgcagtgaac ccgcatggca gggtggtcac aggtgtcctt
       61 tggctgcaca gtaacatgac cgtcatgcat gggacagagg ttgtgagcag catgcgtcga
      121 tgcatacatg cgcatgtagc atgcagcagt gagctccaga gtggaaagct ggcccgctgt
      181 cgtgtgtccg gaccgacagg ccaggctctg gactgggcca tagtcagcag ggcagaggta
      241 ctatgtctag cgccttcctg ggtgtgcagc tttgccttgc catgcttgga catgaatgag
      301 tggggcgcca ggcacttaca agagccacct cgtggtgtgg gcatatggca gaggggcacc
      361 catcagtcgt gtgccattca gaccgtgacg aagcaatcaa tgactgtgct tgtccgggag
      421 aaagtctgtt gtgaaatttg ctgtgatcta ggggccaatg tggcagtcag gaatggctag
      481 agatgctgct agtgtcgtgc atacctggtg tactgccaac agcctcacct gaccatggtg
      541 cttgtgttta aagcaagcat gccacaagtc ggcatgtagg catacggcaa gagctacaca
      601 gttggctgtt gacctgtgga tgcgtgaaac agaagtttga atcgtgccct ttcgcgttga
      661 tgcaccctgt gtgggagcca ggctggtgtg tgtggaagcc aggctggtgg cacacctgtg
      721 tgacctggag gtggacgacc gggtggcgag gcaagtagca gtttcatcaa agaaacacaa
      781 gctgatgcat ccgcgatagt agcacgattc taatgtgtgc acgcaaacgc atcggcccaa
      841 ctggagcagg ggatgcgcag cagcggccgc tggttccacg aactcgaagc gcaaagagca
      901 gtccgctgtg accagcattc cgctagtgtc agggacgata tgaatacggg ttgggcacat
      961 tatgtttggg tcgaggtcgc catggcggac gtgcaagcgg ctgtcgatca cgcctatcgg
     1021 agatgcccag cgcctgcatc gatatgcaac cagctctgca ccagcgcagc aatacgacag
     1081 ctgcagccga tggcctccta gacactgtgc aacaacaaaa aacaattgga ccgtgacggc
     1141 acaggtgtcg aacacagggg ctgagagagg actgcctgtg cttgtgctcc agtgctcata
     1201 cgccatactg atacgcctcc accgatggcc tcactgcctg cggacactgg cagacgctgt
     1261 cgtttggatg tcgcatcgcc atcttcagtg atgttgtggc gttcggcagg atgtacgcat
     1321 gagcgattct tctaaacgag cagtggtggc acatcagcgg ctccctcaaa ccacaatgcc
     1381 ggcttggaag gtagagcatc tgctcggcaa gccgacatag acgatacagc tagcacagcc
     1441 ggcttgcagc tcatcaatca gtacacatac cgtacgtgtc tgaagtatgc cttgttgata
     1501 tgaacactgt accgtggggc cagacgacac ggtctgaagc accgcctctg cagcatctcg
     1561 cacgattgat gcgcatcaat tgcttgcaat tcgatttgca tttggcgcag ttgtctgcac
     1621 aatctttgcc aacgatcgca tgatccgatt caatgatccg aagcatgatc aatacaccgc
     1681 attagcagtg catgcgcgat tgggacacct ctgggggggg atctgatgca cgttcgacac
     1741 gggtgccgaa cctgcgagaa ggctcactac acgcgtgcgg acgcatgcat gcagtgccaa
     1801 agtgcgcatc gcgtgtcaat gctggcgctg ctgacgtccg ctgcctaaac tgcatgaaag
     1861 ctcctgcagt cgcaagtagt ccttttaact tgttgtatcg gaacaaatgg ttgtagtctg
     1921 tgccgtgtga cgatcttgag ccgcctggtc ggcaggtcca atgtcggcct ggaccctgca
     1981 caccctgccc accctgccgc tgctcacccg ttcacgaccc cacaggtgct cgcagcagta
     2041 attgtcgata gatagcaact gtgttctgac tattctttga ccttccgcat ttgggactgc
     2101 ggcggcacac ctactatccg caatgcacac cctggtgctg gtactgcctg cctttccgcg
     2161 cgagcccaca ccatgcaggc gcatagtaat ggactgtgat tgatttaggc aactgtatga
     2221 aacctcttca tcgtgccttg gttgcttgcg ataccctcac gtgagttcat atgaccaacg
     2281 ctggcacgac ccctgcagga tctcgtttga actgcgcgcc agcacattgc agtgcacgcc
     2341 ccgtgccagg cgaaaacgta tttaaccccg aagccagcat actttctcta ccagttcatc
     2401 tcttgttgat ctacctccac cacagtctat cagtgattaa caaacatgag accgaattct
     2461 cgccctatag tgagtcgtat tacctggccg tcgttttaca acgtcgtgac tgggaaaacc
     2521 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2581 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     2641 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     2701 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     2761 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     2821 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     2881 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     2941 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3001 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3061 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     3121 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     3181 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     3241 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     3301 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     3361 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     3421 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     3481 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     3541 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     3601 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     3661 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     3721 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     3781 ccggtgagcg tggctctcga ggtatcattg cagcactggg gccagatggt aagccctccc
     3841 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     3901 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     3961 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4021 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4081 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4141 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4201 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     4261 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4321 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4381 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4441 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4501 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4561 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4621 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4681 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4741 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4801 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     4861 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4921 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4981 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     5041 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacgagctat ttaggtgaca
     5101 ctatagaagt gaagctt
//
