LOCUS       pGG-A-pUM120_0017-B        4490 bp    DNA     circular SYN 27-MAR-2023
DEFINITION  .
ACCESSION   KF718964
VERSION     KF718964.1
KEYWORDS    .
SOURCE      Cloning vector pGGA000 (unknown)
  ORGANISM  Cloning vector pGGA000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2k-c4nnd55"">A</a>"
                     /label="A"
     promoter        12..1815
                     /modified_by="jonasblomme"
                     /modified_by="mamee"
                     /label="pUM120_0017"
     GG              complement(1816..1826)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:28v-ciz6xqo"">B</a>"
                     /label="B"
     primer          complement(1846..1866)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        2346..2450
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2451..3311
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3482..4070
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4434..4455
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc ttgagtgcgc ctctgcctct ttgctccacc tgatctcagc tgtcccaagc
       61 ggtcggtgat gccagcacta ccaacccggc cctggcttag gcatcgtcgt gtccttccgc
      121 ccaggagttg tctcgagcac tgcctcaacg cttccaatgt tgtggctctg aggcctcaac
      181 ggcctggccg tgcggcacaa ctggcaggag acaccacttt tgtggacctg cttctgggtt
      241 ctcgtgtaag cctatggctt gctgccatgc aggttgcatt cagtcgttcc gggcgatggt
      301 tcaggacagg ctgccaggcg tctactcttt ctttcctaca gagtgcttgc acatcaccat
      361 tcgaggcatg tcataaggca gagccggcct gtaggatatc tggcatggaa ggaaacagag
      421 ctctaggtag gcaggttcct ggtcaacttg ccagacttga gtagccatgg cgatttgact
      481 ggccagtacc ttcagggcat tgtacgcaaa tacagatatt attgaacagt aatggtatgc
      541 acaccgggcg ttgacgggca ttcatgcaaa gacacggcac tgcagttggt ccaggaaagg
      601 gcagcttcat cgcagctgca caccagtgct atgtgagtgt tgttttgtgt tgctgccgtg
      661 tcatcttatg tgtaattaca tcgccatgct caaggacatg gcgttgcttg gcttgttgaa
      721 atgtggtcgt aaggttcagg ttattgcgct gtcaagccgc atggttagca ttggtcttgc
      781 agcacggacc aatgaagttg cagccgcgtc agtcatgcag cggatgtggg cacgcggtat
      841 gatgctcatg ttttcattgt tttgtgtgca gcagaatgca ctttaccaga atgtcccatg
      901 ctcagtaacc gggtggattc tgcagcgaca atagccgttg agtgatcact acacatattc
      961 ttgtcagctg ctgtatgcag atggagtgtt gcatacagat ctatgattca atgagaatgc
     1021 tcgttgtagc cacgcaaggc actctgtcac tggccatggt tttgcagcca ccaatctggc
     1081 agctcttcac tggcagacat tgcattgcaa caaggctcca tggagttcca caagccagac
     1141 tacgtgctcc actgaagcca ggtgagtcga cttggctaca agtccgacac cacgacatct
     1201 gcttcaattc gagagcaaca ccagctgtga cctctgtgat catgtacata gcattgtatc
     1261 tgaaagctag cacgtttgac tcgccagagt tacagcgatg tcgcagtaca acgcatgcat
     1321 tgatcattac acgggtaaaa tggtaccatg tcgaagctat atatttttta ttctgcgcca
     1381 ggccaaaatg gtaccggcta gtctgcgccc tcagtaagtg gtcggctagc gtgcagggcg
     1441 gagcgggccg acaagacggg agggtggtcg acttccaggc acgcagaatc gaagcatgcc
     1501 aacagtaccc taatgcagtt tgcgcacaaa agtttcaaaa tccctgattg cgtacacggg
     1561 agaaacgtca caggagcaac ttgcttgcgt acaggggcgg tgacggccac gcacgtgtat
     1621 gctgattcga catgcatgcg gcagcagtgt ccaggtgcag cgcatgcagc cgcaactgtg
     1681 ctcagctgag tcgatactag ccatcgatcg ggcaggacgg cgaattgtat atattccgac
     1741 gggccgaggt gataatgtaa agtttgtagt gcctccaagt tcttcttagc agtcaattct
     1801 ctacaccgca aaaaaaacat gagaccgaat tctcgcccta tagtgagtcg tattacctgg
     1861 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     1921 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     1981 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
     2041 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     2101 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     2161 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     2221 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     2281 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     2341 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2401 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     2461 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     2521 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     2581 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     2641 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     2701 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     2761 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     2821 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     2881 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     2941 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     3001 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3061 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     3121 cggctggctg gtttattgct gataaatctg gagccggtga gcgtggctct cgaggtatca
     3181 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     3241 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     3301 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     3361 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     3421 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3481 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3541 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3601 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3661 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3721 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3781 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3841 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3901 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3961 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4021 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4081 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4141 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4201 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     4261 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     4321 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     4381 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     4441 gataacaatt tcacacgagc tatttaggtg acactataga agtgaagctt
//
