LOCUS       pGG-A-pUM061_0014-B        3748 bp    DNA     circular SYN 27-MAR-2023
DEFINITION  .
ACCESSION   KF718964
VERSION     KF718964.1
KEYWORDS    .
SOURCE      Cloning vector pGGA000 (unknown)
  ORGANISM  Cloning vector pGGA000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2k-c4nnd55"">A</a>"
                     /label="A"
     promoter        12..1073
                     /modified_by="jonasblomme"
                     /modified_by="mamee"
                     /label="pUM061_0014"
     GG              complement(1074..1084)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:28v-ciz6xqo"">B</a>"
                     /label="B"
     primer          complement(1104..1124)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1604..1708
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1709..2569
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2740..3328
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3692..3713
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc taggggtgcg gggaaacggc ggcagggggg gcgaatgcgg ctgcggacgc
       61 ggcggggggc caggcagcgg acggcggtgg ggaggggagg agtgagccgg cggtgcgaat
      121 ggaggtgtta gcacaggcgc cgaataaaaa gtggattgca gccaggtggg cactgggggg
      181 ggagctggtg cacatcgacg acgttgcgac acgggaccgg aactccctgg cgagcgcgga
      241 ccggaacgtt cagggcatgc tgtccacata cttccctggt gtcatggggc tgtgactgat
      301 gtgactgcca agattggaca tgtgtgagga gctcgtggcc aggcgggtgc gattgtgcgg
      361 tgtgagagcc gggtgctaag gacgctgagc ggcgggtgga gtgccaggga caggtgtggc
      421 ttgcgaggat tattatgaat ggctgctggg catggcagaa tggtgatgac gtaggctgtc
      481 atcccgggta ggcaggcgtg atgcatgtgg aatggctggg cttctgcggc ctgcagtaga
      541 atttttcagt gggttgttgg gcacggctgg tccgtgagtg cccacatatg tacctgcagc
      601 tgaggcaatc ctgtgccgtg acggcataga tcagtgtaat tggtgcacaa gatgtgcatc
      661 tcatatgtcc aacatgattc caggcaatgc ctgcatctta ctaagcactt gcagatggtg
      721 aaatgttgcc aggaccaggg ctgccctgta gtcatcgctc tgccgaagca aacctacatc
      781 accaagaaat caaagcaaaa ttataaaccg catctgggct agcttcgcga tgcgataata
      841 tgcgcgaaat gcgccaaggc tgcaccatcc ctgtgtcgcg acacatgtcg cgaccatatg
      901 ttgcgaaagt ggcttggtgc tgaagccgcc atcgcctggg cccccctgaa acactaccag
      961 atgggtcaat tcattcgatc taccaacgac gactgattta tagcattccg caatcactgc
     1021 atctccctct tctaaacatt tcgttagtgc ccgcgtgctt aaactctttc gaaaacatga
     1081 gaccgaattc tcgccctata gtgagtcgta ttacctggcc gtcgttttac aacgtcgtga
     1141 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     1201 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     1261 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     1321 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     1381 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     1441 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     1501 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     1561 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     1621 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     1681 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     1741 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     1801 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     1861 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     1921 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     1981 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     2041 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     2101 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     2161 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     2221 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     2281 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     2341 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     2401 taaatctgga gccggtgagc gtggctctcg aggtatcatt gcagcactgg ggccagatgg
     2461 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     2521 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     2581 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     2641 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2701 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2761 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2821 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2881 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2941 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3001 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3061 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3121 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3181 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3241 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3301 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3361 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3421 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3481 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     3541 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     3601 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     3661 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacgagcta
     3721 tttaggtgac actatagaag tgaagctt
//
