LOCUS       pGG-A-pUM056_0044+RbcsI-B        4487 bp    DNA     circular SYN 27-MAR-2023
DEFINITION  .
ACCESSION   KF718964
VERSION     KF718964.1
KEYWORDS    .
SOURCE      Cloning vector pGGA000 (unknown)
  ORGANISM  Cloning vector pGGA000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2k-c4nnd55"">A</a>"
                     /label="A"
     promoter        12..1315
                     /modified_by="jonasblomme"
                     /modified_by="mamee"
                     /label="pUM056_0044"
     intron          1316..1812
                     /label="RbcsI"
     GG              complement(1813..1823)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:28v-ciz6xqo"">B</a>"
                     /label="B"
     primer          complement(1843..1863)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        2343..2447
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2448..3308
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3479..4067
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4431..4452
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc tcggaagagt cggaacgaca ttaatcaata gctgcagttg atggctgggg
       61 taccagcagc tgcagttgat ggctggggtg caccgcagct ccaggtcgag gtccggtacc
      121 ggtgtcgggg gctgtcgcgg ttgtgggcat gccccgtgaa gccagtaagt atggcttccg
      181 tcccgcaaga gcgagcatgt ccaccgaaaa ttgcttcacg tggcatgacc catagcgagt
      241 gcgccgtagc tccagagtgg tgctctaagt aaccagtcgt ggggtcagca ggacatgtac
      301 atatgtccgg acacagcacg cgcaggattg gaaacatcac cccacgccaa tttcatctac
      361 acagttctga tgtggtaaac tgtcacgact gctgcagcgt cgctcgatct catgatcact
      421 gccccttgac gcagatgaga gtcacgatga caatctgtac cagtgataag tccaaaatta
      481 gcaagtaggt actgcgtggt gtccgtttgg tcgtgttcgc attcctgccc gtgcatgcgc
      541 ccgcatcaat ttcgcccgca acgtacagag tggtcactct gacagctata ggggggaaat
      601 ggtcacccgg gtggccatgc gggcaaaagt cgttcatgag tgttgcgacc actgcccgag
      661 cgctgggcac gtgattggta tttcacttga gctgacattc cgcaacattc cattgcgcta
      721 gagacatgca ggtttacgcg tgccagtggc atggcatggg gaagtcgagc tgtgcgcagc
      781 tgcaggggct ggattcgcgt tgggcccaat tgggctcgga ggccaaatga ttgttttttg
      841 gtgcattgcc atgcatcgtt cctctatgtt ctaccacctt gtggagcata catctgtaac
      901 gtatctcatg aattgggctc accggccgcg cgtggctctg atgcaacata tggcgatcga
      961 ccaatatcgg tggcgcgcgg atcgcgcgcg ccgagcgcat gcaggattca ttcacttgat
     1021 tattcgggga tattcgcagt gaaagatcca gtggggatac tcgttgcccg atcctcaccc
     1081 ccagatacag tcgatcccgc acgcatgcgc acgcatggca ccgccagatc gatcggtgcg
     1141 gatcatgcag gattgtcagg atgcacccca gcaggatcgc gtgtaggcat ggctgcttgg
     1201 tccaaaaaac gacagcatgc ccgtgtgtct gcctgagtgc atatatgcag ctgccgtccg
     1261 cacaatcctt accagaacca aacttcgccg cccacatcga acagcactag taacagtgcg
     1321 catcgcgtgt caatgctggc gctgctgacg tccgctgcct aaactgcatg aaagctcctg
     1381 cagtcgcaag tagtcctttt aacttgttgt atcggaacaa atggttgtag tctgtgccgt
     1441 gtgacgatct tgagccgcct ggtcggcagg tccaatgtcg gcctggaccc tgcacaccct
     1501 gcccaccctg ccgctgctca cccgttcacg accccacagg tgctcgcagc agtaattgtc
     1561 gatagatagc aactgtgttc tgactattct ttgaccttcc gcatttggga ctgcggcggc
     1621 acacctacta tccgcaatgc acaccctggt gctggtactg cctgcctttc cgcgcgagcc
     1681 cacaccatgc aggcgcatag taatggactg tgattgattt aggcaactgt atgaaacctc
     1741 ttcatcgtgc cttggttgct tgcgataccc tcacgtgagt tcatatgacc aacgctggca
     1801 cgacccctgc agaacatgag accgaattct cgccctatag tgagtcgtat tacctggccg
     1861 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     1921 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     1981 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     2041 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     2101 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     2161 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     2221 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     2281 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     2341 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     2401 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     2461 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     2521 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     2581 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     2641 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     2701 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     2761 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     2821 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     2881 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     2941 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3001 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     3061 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     3121 ctggctggtt tattgctgat aaatctggag ccggtgagcg tggctctcga ggtatcattg
     3181 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     3241 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     3301 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     3361 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     3421 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3481 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3541 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3601 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3661 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3721 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3781 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3841 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3901 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3961 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4021 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4081 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     4141 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     4201 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     4261 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     4321 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     4381 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     4441 aacaatttca cacgagctat ttaggtgaca ctatagaagt gaagctt
//
