LOCUS       pGG-A-pUM050_0066-B        4018 bp    DNA     circular SYN 22-MAR-2023
DEFINITION  .
ACCESSION   KF718964
VERSION     KF718964.1
KEYWORDS    .
SOURCE      Cloning vector pGGA000 (unknown)
  ORGANISM  Cloning vector pGGA000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2k-c4nnd55"">A</a>"
                     /label="A"
     promoter        12..1343
                     /modified_by="jonasblomme"
                     /modified_by="mamee"
                     /label="pUM050_0066"
     GG              complement(1344..1354)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:28v-ciz6xqo"">B</a>"
                     /label="B"
     primer          complement(1374..1394)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1874..1978
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1979..2839
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3010..3598
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3962..3983
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc tgcgcttgat gagctcgtgc agctccgcga ggatgggcac cttgcgcagc
       61 tgcatgcggc tgaagtccat ctgccacgcg agctgctttg ggtaccatgg catcgggtga
      121 ggcgcgatgc acgccttgcc tgtacagacc agcatgagat ggggctcaca cgccagacgg
      181 gaagctgcgg gcacgcagca ggcctgccct catggcttcc gtcagctaga gcgggatgcc
      241 gctgtgacgt gtgtcgtttg cagcaagcgg tcggcagcgg ttggggcttg gagtggcacc
      301 ttcaacaatg atcttgccat cagagaacct tgagaagaag tcgttctgca ggcgctcctt
      361 gagctgctcc gagaacttgc ctgagccgtt gatgcggaag gcgatgggca gaggctcccg
      421 cagagtgtgc atgaactgat tccattccgc ttcagggcag acgtgctgtt ccttgtagta
      481 atcttcgaat tcctgattca ggctgccttc ttttgccttt tcgtcaatgt tctcccatgt
      541 tgtgttgcga tttctgtagt ctgtccactc atcgctgcgc ttgcccttcc cgaagtcctt
      601 cttttgagtc cgctgcgact gaagcacacc gcagtgtata tgacactgta gcatccatgc
      661 cggcaaggcg cacatacata cagacatacc ggcgatgcac acatacggac aaatttgttt
      721 gtacttcctg acgtggacgc tgttgatggg tcggtcgtgg ctgtagacca aggcagcgca
      781 gtcgggcggc gtggcagcga tcaggcagct gaatgcggag agaagagcag aaaattcata
      841 ccttggattt gtatttgttg tttctgccca tactcacgca cgcttggtgt aaggaggctg
      901 tgccggccgc ggccaaaacc tgttgcaacg atatggtctg gcggacgacg ctggatggca
      961 caccacgacc aagcatacag gtatgtgcac cgcccttctg acatcggtct gcaaagtggt
     1021 cggactgcgt gcgtgttata ttgattgtcc cgtgtatgcc tattgtgcag ctttccggtg
     1081 gtggccacgc acgcaacgat cacctgacga tcacggccga tcgcggagtc gaccggtgtc
     1141 gaaggggcaa cgtggcgacg ggtgacatgt gatgcatgcg agcagccgaa tcgggaattg
     1201 tatctgctac cgaatcaaca gagcgcacgg gtgatcagcg catcggctgc gagacatata
     1261 atccagtcgt tgcctggccg tggtcacaca cactcgatca ctcctctgca cagcccttca
     1321 cccactttca atttgccgta agtaacatga gaccgaattc tcgccctata gtgagtcgta
     1381 ttacctggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     1441 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     1501 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
     1561 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     1621 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     1681 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     1741 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     1801 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     1861 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1921 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1981 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     2041 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2101 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2161 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2221 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2281 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2341 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2401 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     2461 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     2521 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     2581 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2641 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg
     2701 aggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2761 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2821 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2881 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2941 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3001 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3061 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3121 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3181 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3241 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3301 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3361 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3421 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3481 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3541 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3601 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3661 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3721 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3781 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     3841 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     3901 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     3961 tgtgagcgga taacaatttc acacgagcta tttaggtgac actatagaag tgaagctt
//
