LOCUS       pGG-A-pUM020_0181-B        4686 bp    DNA     circular SYN 22-MAR-2023
DEFINITION  .
ACCESSION   KF718964
VERSION     KF718964.1
KEYWORDS    .
SOURCE      Cloning vector pGGA000 (unknown)
  ORGANISM  Cloning vector pGGA000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2k-c4nnd55"">A</a>"
                     /label="A"
     promoter        12..2011
                     /modified_by="mamee"
                     /label="pUM020_0181"
     GG              complement(2012..2022)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:28v-ciz6xqo"">B</a>"
                     /label="B"
     primer          complement(2042..2062)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        2542..2646
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2647..3507
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3678..4266
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4630..4651
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc tttctcatca taacacgtgg gcggcaacgt tcgatggcat cccaagtatt
       61 tttgggaata catgtgcatg ctgtgtccac gaccaaagcc tgggtaaggc aaacgtactg
      121 atgactttgc cctcgccccc cgctaagcag agtaggtgta caaatcgatg tcaaactgct
      181 ggattgacgt tgtcgatgcc agtgcggtgg cacaggcata acacgtgtga taactattcc
      241 atgcgagttt gatggtcatt ggtgtgtatc gcagagacta atgctccacg aatatacatg
      301 accgtatctg catatgtccg ttcgggatgg gtatggattt tggagctggt gaggagacgc
      361 tgaccccgtt acatcaagct agaacctgag cttcgtgaaa aaccatctgt tcttgccagt
      421 tttgaacttg tcctctaaca atgactttgc ctcctgtaca tgtccatgtt gtagtgaagt
      481 acacccaggg tgtccatcct gggcagcaca cagcattgat gcagacacac aatcacattg
      541 cctccatcat gtcaggagac atgaaaccct gatatgtggc ttgaggacaa aagaatgacg
      601 agggagcaac tctttaagat atgtgcgact agttttgact aatatgcaga gatagcagtt
      661 agttgtgacc ccgagccagg acgtgcgctg cagttgccat gtcactgagc tggcaatcca
      721 gaagcgtgtg tcgttggatt atgtcaacat ggaacagcca aagtggccta gcactgtgtg
      781 actggctgtc catggcagca cgcttatcac caaccctctc cagaccaaag ccacatagct
      841 gctgcaacgc tcgataaatg gccacgaacc tctttcgcca cccgcgactg ctcatacata
      901 agtgagctga tgtaagaggc aagagagaaa atcaccttgt tagcctcttt cttcttctca
      961 gcgttgtcgg ttgtttctgc cgaaatcacc ttcttgaggt caacatcagt caagttgtac
     1021 cgagtgggca taagatgtgt gtagttgaca gtcttaatga aagtctgcat gccgataaac
     1081 tggcttcaac tcggctgaga ctttccacct gacgctagca cgggtgccaa acgacgcaat
     1141 gaccgaaagc ccacaggtag gtgcttcgat atcacacttc aagcacaaat ggaaccgaac
     1201 gcttcgctga aaagcagtta ggctgtgaca aaattttggt gaccagtcca ctaagatgtg
     1261 ctgactgcca tgccacgggc ttcaccgcat gctgcggtga tcagcgtcgc gatttggcgc
     1321 accttcagcg aactcctcct gttctgcttc ttgatggact gtttcttgat tacctaaaag
     1381 gccaccactg tcttggatgc gcggaatgat ggctgccgct cgtaagcctg accagttgtt
     1441 ttcggctgta cacgaagtca atggaacatg ggtcagcacc tttctaggtt ctttggacag
     1501 gccgcagatg agggcatggc cgtatcgccg cgatccggat gcctcgtcgt ggctcttcac
     1561 gatgacagcc ttcttaccgg cgtggcgtcc ttgaaggacg atcacgacct tgccaggctg
     1621 tgttcagaca acaacggtta gaaattcgga agtggtacag gggaaaagga ggggtggaat
     1681 ctcaaaccgg cgaggtgatg tgcgcacctt caggaacttg accattttgc cagaaatacg
     1741 ccaataaagg ccggtcgggt agttgacgag gattttgtgc agcaacaatg gaatgcgtcg
     1801 ccactctggt cctggacgac gttgtggtcc gagcgtcgac ataggcaggg agtacgtcgc
     1861 atcgggggga gggggccttt ggcggcggcg atacattact gccgcgcagc acttcgctaa
     1921 acaactcccg cgaggaacgt cacatatttc agactgcagg acagcttcaa ccctttttcg
     1981 cgtttactgc ggttgtgcaa gcacaggaaa caacatgaga ccgaattctc gccctatagt
     2041 gagtcgtatt acctggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     2101 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     2161 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     2221 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     2281 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     2341 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     2401 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     2461 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     2521 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     2581 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     2641 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     2701 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     2761 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     2821 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     2881 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     2941 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     3001 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     3061 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     3121 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3181 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     3241 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     3301 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     3361 ggctctcgag gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     3421 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     3481 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     3541 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3601 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     3661 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3721 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3781 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3841 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3901 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3961 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4021 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4081 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4141 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4201 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4261 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4321 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     4381 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     4441 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     4501 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     4561 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     4621 tgtggaattg tgagcggata acaatttcac acgagctatt taggtgacac tatagaagtg
     4681 aagctt
//
