LOCUS       pGG-A-pUM020_0181_intron-B        4767 bp    DNA     circular SYN 22-MAR-2023
DEFINITION  .
ACCESSION   KF718964
VERSION     KF718964.1
KEYWORDS    .
SOURCE      Cloning vector pGGA000 (unknown)
  ORGANISM  Cloning vector pGGA000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2k-c4nnd55"">A</a>"
                     /label="A"
     promoter        12..2011
                     /modified_by="mamee"
                     /label="pUM020_0181"
     exon            2012..2014
     intron          2015..2092
     GG              complement(2093..2103)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:28v-ciz6xqo"">B</a>"
                     /label="B"
     primer          complement(2123..2143)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        2623..2727
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2728..3588
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3759..4347
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4711..4732
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc tttctcatca taacacgtgg gcggcaacgt tcgatggcat cccaagtatt
       61 tttgggaata catgtgcatg ctgtgtccac gaccaaagcc tgggtaaggc aaacgtactg
      121 atgactttgc cctcgccccc cgctaagcag agtaggtgta caaatcgatg tcaaactgct
      181 ggattgacgt tgtcgatgcc agtgcggtgg cacaggcata acacgtgtga taactattcc
      241 atgcgagttt gatggtcatt ggtgtgtatc gcagagacta atgctccacg aatatacatg
      301 accgtatctg catatgtccg ttcgggatgg gtatggattt tggagctggt gaggagacgc
      361 tgaccccgtt acatcaagct agaacctgag cttcgtgaaa aaccatctgt tcttgccagt
      421 tttgaacttg tcctctaaca atgactttgc ctcctgtaca tgtccatgtt gtagtgaagt
      481 acacccaggg tgtccatcct gggcagcaca cagcattgat gcagacacac aatcacattg
      541 cctccatcat gtcaggagac atgaaaccct gatatgtggc ttgaggacaa aagaatgacg
      601 agggagcaac tctttaagat atgtgcgact agttttgact aatatgcaga gatagcagtt
      661 agttgtgacc ccgagccagg acgtgcgctg cagttgccat gtcactgagc tggcaatcca
      721 gaagcgtgtg tcgttggatt atgtcaacat ggaacagcca aagtggccta gcactgtgtg
      781 actggctgtc catggcagca cgcttatcac caaccctctc cagaccaaag ccacatagct
      841 gctgcaacgc tcgataaatg gccacgaacc tctttcgcca cccgcgactg ctcatacata
      901 agtgagctga tgtaagaggc aagagagaaa atcaccttgt tagcctcttt cttcttctca
      961 gcgttgtcgg ttgtttctgc cgaaatcacc ttcttgaggt caacatcagt caagttgtac
     1021 cgagtgggca taagatgtgt gtagttgaca gtcttaatga aagtctgcat gccgataaac
     1081 tggcttcaac tcggctgaga ctttccacct gacgctagca cgggtgccaa acgacgcaat
     1141 gaccgaaagc ccacaggtag gtgcttcgat atcacacttc aagcacaaat ggaaccgaac
     1201 gcttcgctga aaagcagtta ggctgtgaca aaattttggt gaccagtcca ctaagatgtg
     1261 ctgactgcca tgccacgggc ttcaccgcat gctgcggtga tcagcgtcgc gatttggcgc
     1321 accttcagcg aactcctcct gttctgcttc ttgatggact gtttcttgat tacctaaaag
     1381 gccaccactg tcttggatgc gcggaatgat ggctgccgct cgtaagcctg accagttgtt
     1441 ttcggctgta cacgaagtca atggaacatg ggtcagcacc tttctaggtt ctttggacag
     1501 gccgcagatg agggcatggc cgtatcgccg cgatccggat gcctcgtcgt ggctcttcac
     1561 gatgacagcc ttcttaccgg cgtggcgtcc ttgaaggacg atcacgacct tgccaggctg
     1621 tgttcagaca acaacggtta gaaattcgga agtggtacag gggaaaagga ggggtggaat
     1681 ctcaaaccgg cgaggtgatg tgcgcacctt caggaacttg accattttgc cagaaatacg
     1741 ccaataaagg ccggtcgggt agttgacgag gattttgtgc agcaacaatg gaatgcgtcg
     1801 ccactctggt cctggacgac gttgtggtcc gagcgtcgac ataggcaggg agtacgtcgc
     1861 atcgggggga gggggccttt ggcggcggcg atacattact gccgcgcagc acttcgctaa
     1921 acaactcccg cgaggaacgt cacatatttc agactgcagg acagcttcaa ccctttttcg
     1981 cgtttactgc ggttgtgcaa gcacaggaaa catggtccgt cgcacactga atcgcatgtc
     2041 ctgttcgcct tgatttgttt gtcacgacaa tcaccttcat gtgctgttgc agaacatgag
     2101 accgaattct cgccctatag tgagtcgtat tacctggccg tcgttttaca acgtcgtgac
     2161 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     2221 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     2281 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     2341 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     2401 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     2461 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     2521 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     2581 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2641 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2701 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     2761 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     2821 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     2881 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     2941 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     3001 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3061 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3121 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     3181 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3241 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     3301 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3361 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3421 aaatctggag ccggtgagcg tggctctcga ggtatcattg cagcactggg gccagatggt
     3481 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     3541 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     3601 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3661 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3721 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3781 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3841 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3901 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3961 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4021 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4081 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4141 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4201 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4261 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4321 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4381 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4441 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     4501 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     4561 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     4621 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     4681 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacgagctat
     4741 ttaggtgaca ctatagaagt gaagctt
//
