LOCUS       pGG-A-pUM020_0016-B        3456 bp    DNA     circular SYN 22-MAR-2023
DEFINITION  .
ACCESSION   KF718964
VERSION     KF718964.1
KEYWORDS    .
SOURCE      Cloning vector pGGA000 (unknown)
  ORGANISM  Cloning vector pGGA000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2k-c4nnd55"">A</a>"
                     /label="A"
     promoter        12..781
                     /modified_by="jonasblomme"
                     /modified_by="mamee"
                     /label="pUM020_0016"
     GG              complement(782..792)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:28v-ciz6xqo"">B</a>"
                     /label="B"
     primer          complement(812..832)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1312..1416
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1417..2277
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2448..3036
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3400..3421
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc ttacgagaga aagatcgaaa agtataaaag gctagcaatg taacaacaca
       61 cgcagacgcg gcagattcac aactggtata cgtgccatct cgcttgatgc tctagagggc
      121 ggacaattag aacgcactac tccgtgaaga ggtccataca aaccttatac gaatttcggg
      181 cagtgtcgat atacggtgcc acgcatgccg tcctcgttgc ggtgccccgc cttgctggtg
      241 tacatccact gcagctggcg agtgcatcct gggcgtagat ccggtaactc ggaactgacg
      301 gtatacaaca gaaaagcgcg cgcaaaccag accaggtgaa tatcttcaga ccatacatgc
      361 gaagaggctt gcactgcatc aattgcgacg ttcactgtgg ctgtggcctt gcgcagagca
      421 gcaggcagct gtcgaagtgg aacagctgtg cactgcctga acggcatcct ctcgcaactt
      481 ccaatcgatc gcaaggcgcc actccatgcc acattataat atcaaaaata tgcatcaggc
      541 tgattttgca atcgcaagag atcgtacagc gtctgtaatg gaatcagata ccctgcccca
      601 ccagtggcta tcggcgctca aacttcgcaa tctcgaaccc agatccgcaa tggatcgaaa
      661 ccagccctga tgctcgcgtc gcacacgccc aggccgagca ctatatatgc cagtgcccaa
      721 cccgtaacct gtaccagacc cgttccaaac tactgataca tcctttcctg cacgcatcac
      781 aaacatgaga ccgaattctc gccctatagt gagtcgtatt acctggccgt cgttttacaa
      841 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      901 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      961 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     1021 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     1081 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     1141 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     1201 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     1261 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     1321 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1381 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     1441 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     1501 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     1561 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     1621 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     1681 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     1741 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     1801 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     1861 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     1921 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     1981 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     2041 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     2101 attgctgata aatctggagc cggtgagcgt ggctctcgag gtatcattgc agcactgggg
     2161 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     2221 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     2281 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     2341 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     2401 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2461 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2521 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2581 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2641 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2701 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2761 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2821 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2881 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2941 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3001 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3061 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3121 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3181 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3241 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3301 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     3361 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     3421 acgagctatt taggtgacac tatagaagtg aagctt
//
