LOCUS       pGG-A-pUM007_0106-B        3675 bp    DNA     circular SYN 22-MAR-2023
DEFINITION  .
ACCESSION   KF718964
VERSION     KF718964.1
KEYWORDS    .
SOURCE      Cloning vector pGGA000 (unknown)
  ORGANISM  Cloning vector pGGA000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2k-c4nnd55"">A</a>"
                     /label="A"
     promoter        12..1000
                     /modified_by="jonasblomme"
                     /modified_by="mamee"
                     /label="pUM007_0106"
     GG              complement(1001..1011)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:28v-ciz6xqo"">B</a>"
                     /label="B"
     primer          complement(1031..1051)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1531..1635
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1636..2496
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2667..3255
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3619..3640
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc tgcacacaga attttccagc aagctgcagt gacgccaagc tgcagctgat
       61 accgacgacg aaggatctta cctcccgaac caagaacccg aaagagggcg atgccgacgt
      121 tagagcgaga cttgacggca cagggatgcg gtgtcgctcc caagccaacg atggtcgtgc
      181 ggggttacgg ggggcttgcc gccctgccct tcaacatgca tggggcgtaa gacgaacagc
      241 ccgcgcgcgt tccagctgtg tgccttcgaa tggactgaaa gtatttgcgc gcaacactca
      301 atggaagctc agttgacagc tgtttttgtt aggttaatct ggcgcaaatc tgagattcag
      361 cgcgtgcgcg cgtacagcat gcgtgcacac acgccctggc ttgaggacat cgatgtcgct
      421 gcggggacaa ctcgtcgcgg cttgcattgg catgccaggt tattcacaca aactgctcaa
      481 tcatttgtga tgtttggtca tacgtccatg ccattgcaca tgaacggccg gtgaatgcac
      541 cgagctttgc tggtttgcgt atacgtggtc gcgatcatgg gatgcaaggt gcacttgtgt
      601 gttttttcaa gagcgccagg acacgtgacc acgaaacacc ccaatgaggt gctcaagaaa
      661 tttgttactt ttgtgtgtgt ttccccgacg ggcaacgcac tctgctgaaa cgtcatgttg
      721 cgctcccttg cctgtcaatg catcaggccc cccaacaact ccccgagtgc ttcgagcgtg
      781 atcacagtct acgagcacct cgcaagactc acgaatcctc aaatccagaa tgacaacaac
      841 cctcttcctc tctttcggtg cgttgatcaa gatgacgacg aggttcagga cccataggaa
      901 gaagcgtggg catgtcagtg ccggccacgg tcgcattggc aagcatcgca agcatccttc
      961 cggtcggggt aatgcaggtc gtcagcatca tcatcgcatt aacatgagac cgaattctcg
     1021 ccctatagtg agtcgtatta cctggccgtc gttttacaac gtcgtgactg ggaaaaccct
     1081 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     1141 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     1201 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     1261 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     1321 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     1381 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     1441 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     1501 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     1561 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     1621 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     1681 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     1741 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     1801 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     1861 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     1921 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     1981 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     2041 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     2101 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     2161 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     2221 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     2281 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     2341 ggtgagcgtg gctctcgagg tatcattgca gcactggggc cagatggtaa gccctcccgt
     2401 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     2461 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     2521 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     2581 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2641 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2701 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2761 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2821 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2881 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2941 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3001 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3061 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3121 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3181 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3241 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3301 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3361 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3421 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3481 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     3541 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     3601 cgtatgttgt gtggaattgt gagcggataa caatttcaca cgagctattt aggtgacact
     3661 atagaagtga agctt
//
