LOCUS       pEN-L1-PJ23119-BsaI-ccdB-CmR-BsaI-Scaf-L2        4116 bp    DNA     circular UNA 22-MAR-2023
DEFINITION  .
ACCESSION   urn.local...8t-9crjciy
VERSION     urn.local...8t-9crjciy
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     attL1           1..100
                     /Color="#00cccc"
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL1"
                     /Transferred_Similarity="99.00%"
                     /Primary_Match="99.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:li-eztyqs1"">attL1</a>"
                     /label="attL1"
     promoter        107..141
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PJ23119"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:1j-cieiaam"">PJ23119</a>"
                     /label="PJ23119"
     CDS             complement(159..464)
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.35%"
                     /Primary_Match="99.35%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:t-cj5879l"">ccdB</a>"
                     /label="ccdB"
     UTR             complement(465..783)
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.37%"
                     /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:thjac:t-cj5879l"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    complement(806..1465)
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="14 CmR"
                     /Transferred_Similarity="99.85%"
                     /Primary_Match="99.85%; Adjusted-Interval-0:0/0; CmR (<a
                     href=""urn:local:.:1h-fz09kes"">14 CmR</a>)"
                     /Alternative_Match="99.85%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     promoter        complement(1519..1549)
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="14 CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:1h-fz09kes"">14 CmR</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacUV5 (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     Scaffold        1585..1667
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpCas9 Scaffold"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Scaffold
                     (<a href=""urn:local:thjac:2f-c4nnd55"">SpCas9
                     Scaffold</a>)"
                     /label="Scaffold"
     attL2           complement(1673..1772)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ll-eztyzh8"">attL2</a>"
                     /label="attL2"
     primer          complement(1810..1830)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        1891..1941
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    1942..2751
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2o-c4nnd56"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2898..3486
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4085..4105
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct taacctttga cagctagctc
      121 agtcctaggt ataatgctag ctgagaccat ccgtcgactt atattcccca gaacatcagg
      181 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata
      241 acggagactg gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata
      301 tgcaccaccg ggtaaagttc acgggagact ttatctgaca gcagacgtgc actggccagg
      361 gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga
      421 cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc agtccctgtt
      481 ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg
      541 attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct
      601 taccagaccg gagatattga catcatatat gccttgagca actgatagct gtcgctgtca
      661 actgtcactg taatacgctg cttcatagca cacctctttt tgacatactt cgggtataca
      721 tatcagtata tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg
      781 cttttagtaa gccggatcca cgcgtttacg ccccgccctg ccactcatcg cagtactgtt
      841 gtaattcatt aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa
      901 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
      961 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     1021 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     1081 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     1141 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     1201 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag
     1261 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     1321 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     1381 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     1441 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgccggat
     1501 cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc
     1561 ctaatgcggc cgcggatggt ctcagtttta gagctagaaa tagcaagtta aaataaggct
     1621 agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttgta ttacccagct
     1681 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     1741 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag
     1801 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     1861 tgttacattg cacaagataa aataatatca tcatgaacaa taaaactgtc tgcttacata
     1921 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     1981 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     2041 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2101 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     2161 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     2221 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     2281 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     2341 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2401 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     2461 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     2521 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2581 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2641 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2701 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     2761 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     2821 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     2881 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2941 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3001 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3061 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3121 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3181 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3241 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3301 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3361 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3421 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3481 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3541 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3601 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3661 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     3721 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
     3781 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     3841 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     3901 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     3961 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
     4021 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
     4081 cgttgtaaaa cgacggccag tcttaagctc gggccc
//
